Alpha amylase from a fungal culture grown on oil cakes and its properties

Ramachandran, Subramaniam; Patel, Anil Kumar; Nampoothiri, K. Madhavan; Sandhya, Chandran; Szakács, George; Soccol, Carlos Ricardo; Pandey, Ashok

doi:10.1590/s1516-89132004000200019

Cited by 82 publications

(48 citation statements)

References 15 publications

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“…None of the metal ions evaluated was required for the activity of amylase from the Rhizobium strain ( (Pandey et al, 2000;Gupta et al, 2003;Ramachandran et al, 2004); in contrast, Chakraborty et al (2000) reported an amylase from Bacillus stearothermophilus whose activity was inhibited by divalent metal ions. Inhibition or no effect in the presence of some mono and trivalent cations (1 mM, 5 mM, 10 mM or 50 mM) were also documented for amylases from Lactobacillus manihotivorans LMG 18010T (Aguilar et al, 2000), Bacillus amyloliquefaciens (Sarikaya and Gürgün, 2000), Bacillus sp.…”

Section: Effect Of Metal Ions On Amylase Activitymentioning

confidence: 99%

Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains

Oliveira

Andrade

2010

Braz. arch. biol. technol.

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Section: Effect Of Metal Ions On Amylase Activitymentioning

confidence: 99%

Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains

Oliveira

Andrade

2010

Braz. arch. biol. technol.

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“…These results are in agreement with results reported by Adejuwon et al (2015), who reported an optimal temperature of amylase production to be 30 0 C for Rhizopus arrhizus. Many workers have also reported that 30 0 C was the optimum temperature for amylase production by several fungal genera and species including Aspergillus flavus (Mukherjee and Majumdar, 1973), Penicilium fellutanum (Ramachandran et al, 2004), Aspergillus sp. JGI 12 (Alva, et al, 2007), Aspergillus niger (Bhattacharya et al, 2012;Dalal et al, 2014Ogbonna et.…”

Section: ] Effect Of Different Culture Media On Amylase Productionmentioning

confidence: 99%

EFFECT OF CULTURE CONDITIONS ON THE PRODUCTION OF AMYLASE ENZYME BY RHIZOPUS ORYZAE (Went & Prins Geerl.).

Chaurasia¹,

Chaurasia²,

Chaurasia³

et al. 2017

IJAR

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Amylases are among the most important enzymes and have great significance in present-day biotechnology. Fungal amylase has large application in food and pharmaceutical industries. Considering these facts, the effect of different culture media, temperature and pH on amylase production by Rhizopus oryzae were studied. The activity of amylase was determined by cup plate assay method as described by Chaurasia et al. (2015). In order to select the best suitable medium, the production of amylase enzyme was determined by growing the Rhizopus oryzae in eight different culture media for 3, 6, 9, 12, 15 and 18 days at 30 0 C temperature. Among eight culture media, Brown's, dextroseasparagine-phosphate, Glucose-dox and Glucose-nitrate media have not favoured the production of amylase as in the culture filtrate of these four media, no trace of amylase enzyme have been detected. While remaining four culture media (i.e. Molisch's, Elliots, Fernando's and Waksman's), Rhizopus oryzae was able to produce amylase enzyme. Amongst these four culture media, Fernando's medium was found to be the best for the production of amylase enzyme. Nine days of incubation period was also found to be the best for maxium production of amylase enzyme. For further study, Fernando's medium and nine days of incubation period were selected for amylase production. To study the influence of temperature, Rhizopus oryzae was cultured on Fernando's medium (pH 5.0) for nine days at different temperatures viz., 15, 20, 25, 30, 35, 40, 45 and 50 0 C. The Rhizopus oryzae was able to produce the amylase enzyme at wide range of temperature, ranging from 15 0 to 40 0 C. The 30 0 C temperature was found to be optimum for the maxiumum production of amylase enzyme. Above 40 0 C, no trace of amylase production was recorded. To study the effect of pH on amylase production, Rhizopus oryzae was cultured on Fernando's medium having different pH values, viz, 3.0, 4.0, 5.0, 6.0, 7.0 and 8.0 at 30 0 C for 9 days incubation period. The isolated pathogen Rhizopus oryzae was able to synthesized amylase enzyme at a wide range of pH, i.e., from pH 3.0 to 8.0. Extremely acidic pH (i.e. at pH 3.0), the activity of amylase was found to be less which increased gradually with increase in pH up to pH 5.0. Further

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“…They are very extensively used in beverages, baby foods and pharmaceutical industries. Besides their use in starch saccharification, they also find application in brewing, paper and distillery industries (Ramachandran et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Optimization of process parameters for -amylase production under solid-state fermentation by Bacillus Cereus MTCC 10202

Kalaiarasi¹,

Parvatham²

2013

Afr. J. Microbiol. Res.

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An α-amylase producing bacterial strain was isolated from sago factory effluent discharged soil and identified as Bacillus cereus. Production of extracellular α-amylase by B. cereus was studied in solidstate fermentation. Different substrates like wheat bran, maize bran, corn bran, millet bran, rice bran, greengram bran, blackgram bran, cassava peel powder, cotton seed oil cake, coconut oil cake, sesame oil cake and groundnut oil cake were screened to select the suitable substrate for the production of α-amylase. Among the agro-wastes, wheat bran was found to be the best substrate. Various physical and chemical parameters were optimized. Maximum α-amylase yield was achieved at pH 7 with inoculum level of 10% at 50°C and an incubation period of 72 h. The optimum ratio of substrate to moisture level was found to be 1:2 and substrate weight to flask volume was 1:50. Supplementation of starch at 1% (w/w) concentration and yeast extract at 1.5% (w/w) concentration resulted in maximum production of α-amylase. Calcium chloride at a concentration of 1% (w/w) was found to stimulate α-amylase production. Thus, B. cereus produced a high titre of α-amylase in solid state fermentation using inexpensive agroresidue under optimized conditions.

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Alpha amylase from a fungal culture grown on oil cakes and its properties

Cited by 82 publications

References 15 publications

Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains

Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains

EFFECT OF CULTURE CONDITIONS ON THE PRODUCTION OF AMYLASE ENZYME BY RHIZOPUS ORYZAE (Went & Prins Geerl.).

Optimization of process parameters for -amylase production under solid-state fermentation by Bacillus Cereus MTCC 10202

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