All solid-state GFP sensor

Abstract

An all‐solid‐state green fluorescent protein (GFP) sensor for GFP measurement was developed. It is immune to interference from ambient light and works with standard flow‐through cuvettes. The sensor is practically insensitive to the scattered excitation light encountered in microbial suspensions. It has a range of 0.0002–1 g/L (7.4 × 10−9 – 3.7 × 10−5 M) with limit of detection 0.00019 g/L (7.0 × 10−9 M). The sensor could be used with a UV or blue light emitting diode (LED) as a light source, depending on required sensitivity, selectivity, and background levels. Its very low cost makes it useful in a variety of applications. This article describes the construction and validation of the sensor both off‐ and on‐line in fermentation processes. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 473–477, 2000.

Rapid non-invasive monitoring of baculovirus infection for insect larvae using green fluorescent protein reporter under early-to-late promoter and a GFP-specific optical probe

“…Further, the quantity of GFP directly related to the amount of recombinant protein [8,9,11] and facilitated the selection of elution fractions, which contain the recombinant product [10]. We also showed that a GFP-specific optical probe [17] can be successfully employed to quantitatively detect GFP level in insect larvae system [11]. By using a baculovirus early-to-late (ETL) promoter that is activated early in the infection cycle and is of medium strength [18] instead of a conventional very late polyhedrin (Polh) promoter that is of strong strength, we demonstrated that early monitoring of baculovirus infection for insect Sf-9 cell system is possible [19].…”

“…Each larva at the time of collection was individually weighed and then measured using the GFP-specific optical probe [17] using the experimental setup (Fig. 2) [11].…”

“…A 96-well assay plate (Costar, USA) was used to mix and measure samples. For each sample, 5 ml of homogenized larvae was added [17]. The signal is registered as volts using a digital multimeter (b).…”

Applicability of GFP Microbial Whole Cell Biosensors to Bioreactor Operations - Mathematical Modeling and Related Experimental Tools

“…Basically, GFP-related fluorescence can be monitored non-invasively and in a non-destructive way by a lot of equipments comprising excitation sources and appropriate photomultipliers (Randers-Eichhorn, 1997, Kostov, 2000. However, there are more and more GFP measurements carried out with flow cytometer (Patkar, 2002, Galbraith, 1999, Tracy, 2010, Diaz, 2010.…”

Optical tracking of a stress-responsive gene amplifier applied to cell-based biosensing and the study of synthetic architectures

“…doi:10.1016/j.bios.2009.12.036 candidates for miniature or remotely deployable assays. Namely, high reporter stability, signal accumulation, absence of a requirement for accessory substrates, and the non-invasiveness of the optical probing enable remote or miniature sensing applications (Baumstark-Khan et al, 2001, 2003Belkin, 2003;Knight et al, 1999;Kostov et al, 2000;Kostrzynska et al, 2002;Kuang et al, 2004;Lichtenberg-Fraté et al, 2003;Norman et al, 2005;Rabbow et al, 2003;Sagi et al, 2003;Zanzotto et al, 2006).…”