Rapid non-invasive monitoring of baculovirus infection for insect larvae using green fluorescent protein reporter under early-to-late promoter and a GFP-specific optical probe

Dalal, Nimish G.; Joon, Hyung; Kramer, Shannon F.; Kostov, Yordan; Rao, Govind; Bentley, William E.

doi:10.1016/j.procbio.2005.10.020

Cited by 5 publications

(3 citation statements)

References 19 publications

(42 reference statements)

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“…3e and f). The highest Log(RLU) (7.48) and OD (9.25) were found to be maximum when the inoculums concentration and temperature were 3.23 ml/l (coded value 0.233) and 27.65 • C (coded value −0.47), respectively ( Fig. 3e and f).…”

Section: Effect Of Inoculum Concentration and Temperature On Luminescmentioning

confidence: 94%

“…The other advantages of bioluminescence tests are: high sensitivity to toxic substances, simple measurement procedure, the availability of reagents and bioluminometers, and the possibility of automating the analysis due to use of immobilized reagents [25]. The optical based bio-sensing systems have also been applied for the detection of baculovirus infection, Vitamin B 12 and methyl parathion owing to their high sensitivity [26][27][28][29]. Various bioluminescent media have been reported in literature for the proper growth and luminescence of bioluminescent bacteria such as Boss media, luminous media, sea water broth, and complex media.…”

Section: Introductionmentioning

confidence: 99%

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Development of immobilized biophotonic beads consisting of Photobacterium leiognathi for the detection of heavy metals and pesticide

Ranjan

Rastogi

Thakur

2012

Journal of Hazardous Materials

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Section: Effect Of Inoculum Concentration and Temperature On Luminescmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Development of immobilized biophotonic beads consisting of Photobacterium leiognathi for the detection of heavy metals and pesticide

Ranjan

Rastogi

Thakur

2012

Journal of Hazardous Materials

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“…In addition, the proteins produced using the earlier promoter showed less aggregation in some cases, when compared to proteins produced under the polh promoter. Other applications which have exploited the ability to temporally control the transcription of genes include the production of reporter proteins under the control of the medium strength early-to late (ETL) promoter for monitoring baculovirus infection in insect cell cultures (Dalal et al, 2005(Dalal et al, , 2006. In addition, groups have attempted to increase transcription at earlier times post-infection by the use of a hybrid of the vp39 capsid protein and polh promoters (Pcappolh) (Thiem and Miller, 1990), tandem Ie-1 promoters (Kojima et al, 2001), synthetic late promoters (Blissard et al, 1992), and constitutive promoters such as hsp70 (Lu et al, 1996;Prikhod'ko et al, 1998).…”

Section: Promoter Choicementioning

confidence: 99%

Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks

Sokolenko

George

Wagner

et al. 2012

Biotechnology Advances

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The baculovirus expression vector system (BEVS) is a versatile and powerful platform for protein expression in insect cells. With the ability to approach similar post-translational modifications as in mammalian cells, the BEVS offers a number of advantages including high levels of expression as well as an inherent safety during manufacture and of the final product. Many BEVS products include proteins and protein complexes that require expression from more than one gene. This review examines the expression strategies that have been used to this end and focuses on the distinguishing features between those that make use of single polycistronic baculovirus (co-expression) and those that use multiple monocistronic baculoviruses (co-infection). Three major areas in which researchers have been able to take advantage of co-expression/co-infection are addressed, including compound structure-function studies, insect cell functionality augmentation, and VLP production. The core of the review discusses the parameters of interest for co-infection and co-expression with time of infection (TOI) and multiplicity of infection (MOI) highlighted for the former and the choice of promoter for the latter. In addition, an overview of modeling approaches is presented, with a suggested trajectory for future exploration. The review concludes with an examination of the gaps that still remain in co-expression/co-infection knowledge and practice.

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Optical Instrumentation for Bioprocess Monitoring

Lam

Kostov

2009

Optical Sensor Systems in Biotechnology

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In this chapter the optical sensors for oxygen, pH, carbondioxide and optical density (OD) which are essential for bioprocess monitoring are introduced, their measurement principles are explained and their realization and applications are shown. In addition sensors for ethanol and GFP are presented. With the exception of the optical density sensor all others employ certain fluorophores that are sensitive to the designated parameter. These fluorophores along with their optical properties, the sensing mechanisms and their mathematical formulations are described. An important part of this chapter covers the development of the optoelectronic hardware for low cost systems that are able to measure the fluorescence lifetime and fluorescence intensity ratio. The employment of these probes in the bioprocess monitoring is demonstrated in different fermentation examples.

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Rapid non-invasive monitoring of baculovirus infection for insect larvae using green fluorescent protein reporter under early-to-late promoter and a GFP-specific optical probe

Cited by 5 publications

References 19 publications

Development of immobilized biophotonic beads consisting of Photobacterium leiognathi for the detection of heavy metals and pesticide

Development of immobilized biophotonic beads consisting of Photobacterium leiognathi for the detection of heavy metals and pesticide

Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks

Optical Instrumentation for Bioprocess Monitoring

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