Optical tracking of a stress-responsive gene amplifier applied to cell-based biosensing and the study of synthetic architectures

Martineau, Rhett L.; Stout, Valerie; Towe, Bruce C.

doi:10.1016/j.bios.2009.12.036

Cited by 7 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Modified from [34][35][36] currently promise to be highly rewarding: the increased availability of highthroughput automation, the rapidly developing field of synthetic biology, and the use of bioinformatics and in silico design. Singly or in combination, these approaches can help target the genetic element to be modified, outline the preferred modifications to be introduced, and provide the technical ability to do so.…”

Section: Discussionmentioning

confidence: 99%

“…This improved biosensor was highly specific to cadmium (II) and did not respond to other valence II cations. A synthetic regulatory construct based on a two-stage amplifying promoter cascade was applied to whole-cell biosensing of DNA-damaging agents, based on the E. coli recA and tac gene promoters, fused to green (GFP) and red (mCherry, RFP) fluorescent protein genes, respectively [36]. The two promoters were linked by the LacI repressor in E. coli, where DNA-damage activates the recA promoter and hence the upregulation of GFP and LacI (Fig.…”

Section: Additional Genetic Manipulations and The Advent Of Syntheticmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Manipulations for Enhancing Luminescent Bioreporters Performance in the Detection of Toxic Chemicals

Yagur‐Kroll

Belkin

2014

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

Microbial whole-cell bioreporters are genetically modified microorganisms that produce a quantifiable output in response to the presence of toxic chemicals or other stress factors. These bioreporters harbor a genetic fusion between a sensing element (usually a gene regulatory element responsive to the target) and a reporter element, the product of which may be quantitatively monitored either by its presence or by its activity. In this chapter we review genetic manipulations undertaken in order to improve bioluminescent bioreporter performance by increasing luminescent output, lowering the limit of detection, and shortening the response time. We describe molecular manipulations applied to all aspects of whole-cell bioreporters: the host strain, the expression system, the sensing element, and the reporter element. The molecular construction of whole-cell luminescent bioreporters, harboring fusions of gene promoter elements to reporter genes, has been around for over three decades; in most cases, these two genetic elements are combined "as is." This chapter outlines diverse molecular manipulations for enhancing the performance of such sensors.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Additional Genetic Manipulations and The Advent Of Syntheticmentioning

confidence: 99%

Molecular Manipulations for Enhancing Luminescent Bioreporters Performance in the Detection of Toxic Chemicals

Yagur‐Kroll

Belkin

2014

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

show abstract

“…For example a biosensor based on CrkII adaptor protein that detects the tyrosine kinase activity of Bcr-Abl in cells of CML patients has been developed to study the response of therapy and to detect drug resistant cells within heterogeneous population [15]. A two component tracking strategy involving green fluorescent protein and red fluorescent protein based two stage amplifying protein cascade construct for biosensing DNA damage exhibited increased sensitivity [16]. Quorum sensing in microorganisms is a one of the attributes used by transcriptional biosensors in which small signalling molecules are used to determine their population.…”

Section: Application Of Synthetic Biology In Diagnostic Biosensorsmentioning

confidence: 99%

Synthetic Biology Based Biosensors and the Emerging Governance Issues

Bhatia

Chugh²

2013

Curr Synthetic Sys Biol

View full text Add to dashboard Cite

Synthetic biology is a nascent field of applied science that has found applications in diverse areas such as healthcare, energy, agriculture, food additives and industrial chemicals. Its market is estimated to grow to a value of $4.5 billion in 2015. The role of synthetic biology for development of biosensors for biomedical application and its related governance issues have been considered in the present review. The recent developments in the synthetic biology based biosensors as well as critical factors such as biosafety, standardization and proprietary rights for its socioeconomic acceptance have been discussed. Scrutiny of such issues aims to understand the different parameters that can stimulate the growth as well as success of synthetic biology derived biosensors and minimise the associated risk.

show abstract

Fluorescence‐Based Biosensors

Schäferling

2016

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Biosensors, as defined by Pure and Applied Chemistry, are ‘chemical sensors in which the recognition system utilizes a biochemical mechanism. The biological recognition system translates information from the biochemical domain, usually an analyte concentration, into a chemical or physical output signal with a defined sensitivity’.(1)It is also appointed that chemical or biological sensors contain two basic components connected in series: a chemical or biomolecular recognition system (receptor) and a physicochemical transducer. According to this prerequisite, this overlook is confined to sensor devices that combine a biomolecular recognition element with an optical signal transducer. Homogeneous or intracellular assays using fluorescent molecular probes or nanoparticles are not considered, although they are frequently termed as molecular sensors or nanosensors in the literature.Fluorescence‐based biosensors are generalized as those devices that derive an analytical signal from a photoluminescent (either fluorescence or phosphorescence) emission process. Chemi‐ or bioluminescent detection systems are only briefly discussed in this article.Biosensors are used for a wide variety of tasks, including detection of compounds of biomedical,(2)environmental,(3)or defense interest(4); online monitoring for processcontrol(5); quality control of foodstuffs(6); selective detection of compounds undergoing a chemical separation(7); and screening of drug compounds.(8)Advantages of such devices include high selectivity, rapid response times, reusability, amenability to remote analysis, and immunity to electrical interferences.(9)The selective nature of complexation between biomolecule and analyte and the small size of sensor devices can be combined with advanced detection techiques such as total internal reflection (TIR) spectroscopy.(10)This results in an ability to measure analytes in complex matrices with unsurpassed sensitivity. Such samples may include highly scattering components such as milk or whole blood(11)or relatively inaccessible locations such as groundwater wells, or even intracellular environments.(12)The key limitation of such devices mainly centers on the poor stability of biological compounds, which can lead to a substantial drift in instrumental response over time. The so‐called Cambridge definition appoints another characteristic property of sensors.(13)Therein, they are defined as ‘miniaturized devices that can deliver real‐time and on‐line information on the presence of specific compounds or ions in even complex samples.’ Accordingly, a sensor is expected to respond reversibly and continuously. With the exception of some enzymatic sensors, these conditions are not fulfilled in case of most biosensors. Particularly, in devices where immunological reagents or DNA are used as recognition elements, they show a lack of reversibility and operate only as a ‘one‐shot’ screen, without the potential for continuous, quantitative analysis. Nevertheless, the designations immunosensors or DNA sensors became accepted for such analytical or diagnostic tools.

show abstract

Optical tracking of a stress-responsive gene amplifier applied to cell-based biosensing and the study of synthetic architectures

Cited by 7 publications

References 44 publications

Molecular Manipulations for Enhancing Luminescent Bioreporters Performance in the Detection of Toxic Chemicals

Molecular Manipulations for Enhancing Luminescent Bioreporters Performance in the Detection of Toxic Chemicals

Synthetic Biology Based Biosensors and the Emerging Governance Issues

Fluorescence‐Based Biosensors

Contact Info

Product

Resources

About