Aldehyde functionalized ionic liquid on electrochemically reduced graphene oxide as a versatile platform for covalent immobilization of biomolecules and biosensing

Manoj, Devaraj; Theyagarajan, K.; Saravanakumar, Duraisamy; Senthilkumar, Sellappan; Thenmozhi, Kathavarayan

doi:10.1016/j.bios.2017.12.030

Cited by 54 publications

(22 citation statements)

References 59 publications

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“…Moreover, as expected, the subsequent drop-casting of GOx manifested an increase of R ct with respect to the PtNPs-PAA-aSPCE electrode (432.2 Ω), although this value is ten times lower than that obtained for the bare SPCE. The increment in R ct after the addition of the enzyme suggests a hindrance for electron transfer through the dense protein layer of the enzyme [33,34]. This is in agreement with the LSV features of Figure 1, where PtNPs-PAA-aSPCE depicts a less resistive voltammetric behavior compared with that shown for GOx-PtNPs-PAA-aSPCE (in the absence of H 2 O 2 ), although the immobilized protein on PtNPs resulted in a slight increase in the current intensity at 0.2 V (after background subtraction).…”

Section: Characterization and Optimization Of The Electrochemical Permentioning

confidence: 99%

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Jiménez-Fiérrez

González-Sánchez

Jiménez‐Pérez

et al. 2020

Sensors

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Herein, a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on a surface containing platinum nanoparticles (PtNPs) electrodeposited on poly(Azure A) (PAA) previously electropolymerized on activated screen-printed carbon electrodes (GOx-PtNPs-PAA-aSPCEs) is reported. The resulting electrochemical biosensor was validated towards glucose oxidation in real samples and further electrochemical measurement associated with the generated H2O2. The electrochemical biosensor showed an excellent sensitivity (42.7 μA mM−1 cm−2), limit of detection (7.6 μM), linear range (20 μM–2.3 mM), and good selectivity towards glucose determination. Furthermore, and most importantly, the detection of glucose was performed at a low potential (0.2 V vs. Ag). The high performance of the electrochemical biosensor was explained through surface exploration using field emission SEM, XPS, and impedance measurements. The electrochemical biosensor was successfully applied to glucose quantification in several real samples (commercial juices and a plant cell culture medium), exhibiting a high accuracy when compared with a classical spectrophotometric method. This electrochemical biosensor can be easily prepared and opens up a good alternative in the development of new sensitive glucose sensors.

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Section: Characterization and Optimization Of The Electrochemical Permentioning

confidence: 99%

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Jiménez-Fiérrez

González-Sánchez

Jiménez‐Pérez

et al. 2020

Sensors

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“…Hitherto, aldehyde functionalized ILs (CHO-IL) have been used frequently for the fabrication of ultrasensitive sensors and biosensors. [30][31][32] The aldehydic group of IL can serve as a host for attaching enzymes, biomolecules, and antibodies directly. However, the electrode surface modification or sorption affinity between transducing materials (GO, CNT, MoS 2 , and MXNSs) and ILs through simple physical interactions always suffers from poor stability and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…The aldehydic groups of AFBPB can directly capture enzymes or biomolecules through a strong covalent bond. [31] In addition, the amine groups (NH 2 ) of AFBPB can bind strongly with the hydroxyl OH groups of MXNSs through electrostatic interactions and π-π stacking. [33,34] To the best of our knowledge, studies regarding multifunctional TSIL (AFBPB)-based immunosensing tags in the development of sensors or biosensors have not been published.…”

Section: Introductionmentioning

confidence: 99%

An Electrodeposited MXene‐Ti₃C₂T_x Nanosheets Functionalized by Task‐Specific Ionic Liquid for Simultaneous and Multiplexed Detection of Bladder Cancer Biomarkers

Sharifuzzaman

Barman

Zahed

et al. 2020

Small

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MXenes (with chemical formula M n+1 X n T x , where M = transition metals such as Ti, Sc, V, Nb, Mo, and Cr; X = C and/or N; T x = surface functional groups, such as OH, O, and minor F; n = 1, 2, or 3) are synthesized by selectively etching Agroup sp elements (typically group III A or IV A such as Al or Ga) from layered parent ternary MAX phases (with chemical formula Mn + 1AXn, where, A = elements from groups 13 and 14, such as Al, Ga, C, and Si). [3] To date, families of MXenes comprising more than 30 members have been successfully synthesized, such as Ti 2 CT x , V 2 CT x , Mo 2 CT x , and Ti 3 C 2 T x. [3] Among them, MXene-Ti 3 C 2 T x nanosheets (MXNSs) is the most promising and wellstudied candidate owing to its optimized synthesis, high metallic conductivity, high strength, high stability, excellent catalytic properties, and hydrophilicity with various surface functionalities. [1,4,5] Owing to its versatile chemistry, MXNSs is a promising candidate in a plethora of applications, including supercapacitors, energy storage, [6,7] electronics, [8] and catalysis, [5] In addition, MXNSs have recently emerged as excellent transducing electrode material in electrochemical biosensing owing to its active inherent electrochemistry compared with other 2D nanomaterials. Hitherto, several biosensors based on MXNSs have been utilized for the detection of prostate specific antigen, [9] phenol, [10] microRNA-182, [11] glucose, [12] acetaminophen, and isoniazid. [13] In the fabrication of advanced biosensors, the most challenging issue of arranging nanomaterials into a particular position on a patterned electrode with controlled architecture, morphology, and thickness should be solved. Many chemicals, as well as physical deposition techniques, have been established to assemble 2D nanomaterials (graphene, CNT, MoS 2 , and MXene) over electrodes. Currently, the most popular approaches for assembling 2D nanomaterials onto electrodes for sensing rely on chemical deposition techniques, for example, layer-by-layer assembly, [14,15] spin coating, [16] inkjet printing, [17] spray coating, [18] vacuum filtration, [19] and Controlled deposition of 2D multilayered nanomaterials onto different electrodes to design a highly sensitive biosensing platform utilizing their active inherent electrochemistry is extremely challenging. Herein, a green, facile, and cost-effective one-pot deposition mechanism of 2D MXene-Ti 3 C 2 T x nanosheets (MXNSs) onto conductive electrodes within few minutes via electroplating (termed electroMXenition) is reported for the first time. The redox reaction in the colloidal MXNS solution under the effect of a constant applied potential generates an electric field, which drives the nanoparticles toward a specific electrode interface such that they are cathodically electroplated. A task-specific ionic liquid, that is, 4-amino-1-(4-formyl-benzyl) pyridinium bromide (AFBPB), is exploited as a multiplex host arena for the substantial immobilization of MXNSs and covalent binding of antibodies. A miniaturized, singl...

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“…Thus CMEs prepared with different types of mediators have been reported for the determination of H 2 O 2 . For example, some copper complexes with different ligands such as 2,2‐bipyridyl, 1,10‐phenanthroline, poly‐melamine, 2,4,6‐tris(2‐pyridyl)‐1,3,5‐triazine and DNA with poly(allylamine); azine type dyes such as methylene blue, meldola blue, nile blue, azure A, thionin and ferrocene/thionin (as a bimediator); antraquinone and hydroquinone derivatives; cytocrome C; Prussian blue [PB); poly‐hemin and hemoglobin with MoS 2 and AuNPs etc. have been reported for the electrocatalytic reduction of H 2 O 2 .…”

Section: Introductionmentioning

confidence: 99%

A New Redox Mediator (Cupric‐Neocuproine Complex)‐ Modified Pencil Graphite Electrode for the Electrocatalytic Oxidation of H₂O₂: A Flow Injection Amperometric Sensor

2020

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This paper introduces the cupric‐neocuproine complex ([Cu(Nc)2]2+) as a new redox mediator for the electrocatalytic oxidation of H2O2. For this purpose, [Cu(Nc)2]2+ was modified onto a disposable pencil graphite electrode (PGE) surface by using Nafion (Nf) as a cation‐exchange membrane for the effective adsorption of complex. The modified electrode was prepared by immersing Nf‐adsorbed PGE into [Cu(Nc)2]2+ complex solution (0.40 mM of Cu2+ and 0.80 mM of Nc prepared at pH 4.76 in 0.10 M acetate buffer). SEM images and EDX spectra of electrodes were presented for the elucidation of their surface morphologies. Cyclic voltammetric results showed that H2O2 was electrocatalytically oxidized at [Cu(Nc)2]2+/Nf/PGE, because both the increase in current and the potential shift to a more negative direction were observed for oxidation of H2O2 at [Cu(Nc)2]2+/Nf/PGE in comparison to bare PGE. Then, the electrocatalytic activity of [Cu(Nc)2]2+/Nf/PGE toward H2O2 oxidation was utilized for amperometric determination of H2O2 in flow injection analysis (FIA) system. Flow injection (FI) amperometric studies showed two linear calibration ranges (1.0‐1000.0 μM (R2=0.9958) and 1000.0‐10000.0 μM (R2=0.9902) with a detection limit of 0.4 μM H2O2 which is considered good for electroanalytical determinations, especially for electrocatalytic oxidation. To test the applicability of the developed FI amperometric hydrogen peroxide sensor, H2O2 contents of some relevant samples were determined.

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Aldehyde functionalized ionic liquid on electrochemically reduced graphene oxide as a versatile platform for covalent immobilization of biomolecules and biosensing

Cited by 54 publications

References 59 publications

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

An Electrodeposited MXene‐Ti₃C₂T_x Nanosheets Functionalized by Task‐Specific Ionic Liquid for Simultaneous and Multiplexed Detection of Bladder Cancer Biomarkers

A New Redox Mediator (Cupric‐Neocuproine Complex)‐ Modified Pencil Graphite Electrode for the Electrocatalytic Oxidation of H₂O₂: A Flow Injection Amperometric Sensor

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Aldehyde functionalized ionic liquid on electrochemically reduced graphene oxide as a versatile platform for covalent immobilization of biomolecules and biosensing

Cited by 54 publications

References 59 publications

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

An Electrodeposited MXene‐Ti3C2Tx Nanosheets Functionalized by Task‐Specific Ionic Liquid for Simultaneous and Multiplexed Detection of Bladder Cancer Biomarkers

A New Redox Mediator (Cupric‐Neocuproine Complex)‐ Modified Pencil Graphite Electrode for the Electrocatalytic Oxidation of H2O2: A Flow Injection Amperometric Sensor

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An Electrodeposited MXene‐Ti₃C₂T_x Nanosheets Functionalized by Task‐Specific Ionic Liquid for Simultaneous and Multiplexed Detection of Bladder Cancer Biomarkers

A New Redox Mediator (Cupric‐Neocuproine Complex)‐ Modified Pencil Graphite Electrode for the Electrocatalytic Oxidation of H₂O₂: A Flow Injection Amperometric Sensor