Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Abstract

Herein, a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on a surface containing platinum nanoparticles (PtNPs) electrodeposited on poly(Azure A) (PAA) previously electropolymerized on activated screen-printed carbon electrodes (GOx-PtNPs-PAA-aSPCEs) is reported. The resulting electrochemical biosensor was validated towards glucose oxidation in real samples and further electrochemical measurement associated with the generated H2O2. The electrochemical biosensor showed an excellent sensitivity (42.7 μA mM−1 cm−2), limit of detection (7.6 μM), linear range (20 μM–2.3 mM), and good selectivity towards glucose determination. Furthermore, and most importantly, the detection of glucose was performed at a low potential (0.2 V vs. Ag). The high performance of the electrochemical biosensor was explained through surface exploration using field emission SEM, XPS, and impedance measurements. The electrochemical biosensor was successfully applied to glucose quantification in several real samples (commercial juices and a plant cell culture medium), exhibiting a high accuracy when compared with a classical spectrophotometric method. This electrochemical biosensor can be easily prepared and opens up a good alternative in the development of new sensitive glucose sensors.

Synthesis of NiCo2O4 Nanostructures and Their Electrochemial Properties for Glucose Detection

“…The selectivity of NCOs is also an important factor for accurate glucose detection: current response to other reagents can detract from the determination of glucose [ 47 , 48 , 49 ]. The selectivity of the NCOs depending on different pH was investigated as shown in Figure 5 .…”

Biopolymer Cooperation for Sustainable High‐Performance Oxidase‐Based Biosensing with the Simplest Possible Readout of Substrate Conversion

“…The analytical performance characteristics of a few other recently forwarded glucose biosensors with anodic H 2 O 2 and low µ m detection capability are listed together with the features of the alternative of this study in Table 1 . [ 13–18 ] Comparison of the tabulated values confirms the competitiveness of a biosensor architecture in which a combination of the biopolymers zein and gelatin enables surface‐exposed GOX immobilization with interference protection, resulting in highly effective analysis of low glucose levels.…”

Electrochemical Biosensors Based on Conducting Polymers: A Review

“…The resulting biosensor exhibited very high sensitivity because of the synergistic catalytic activity between polyaniline, multiwall nanotubes and Pt nanoparticles. Another electrochemical glucose biosensor based on glucose oxidase immobilized on a surface containing Pt nanoparticles electrodeposited on conducting poly(Azure A) previously electropolymerized on activated screen-printed carbon electrodes has been developed [108]. The resulting biosensor was validated towards glucose oxidation in real samples and further electrochemical measurement associated with the generated H 2 O 2 (Figure 3).…”

“…SPCE: Screen-printed carbon electrodes, aSPCE: activated SPCE, PAA: Poly(Azur A), PtNPs: Platinum Nanoparticles, GOx: glucose oxidase. Reproduced with permission of[108], Copyright 2020, MDPI.…”