Agrobacterium tumefaciens -mediated transformation of Eucalyptus camaldulensis and production of transgenic plants

Ho, C.-K.; Chang, Shujun; Tsay, Jeen-Yin; Tsai, Chung‐Jui; Chiang, Vincent L.; Chen, Z.-Z.

doi:10.1007/s002990050464

Cited by 79 publications

(52 citation statements)

References 16 publications

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“…Among the combinations of the tested growth substances, those of 2.7 or 5.4 µmol L -1 NAA and 4.44 µmol L -1 BAP yielded best results ( Figure 1B and C); regression curves indicated optimum values when the concentration of BAP was between 4.44 and 8.88 µmol L -1 . These results differ from those described by Ho et al (1998) and Diallo & Duhoux (1984), who obtained bud regeneration on cotyledonary leaf explants cultured in the presence of 16.2 µmol L -1 NAA and 4.44 µmol L -1 BAP or 5.4 µmol L -1 NAA and 2.22 µmol L -1 BAP. These authors used auxin concentration higher than the BAP concentration.…”

Section: Effect Of the Combinations Of Naa And Bap On Callogenesis Ancontrasting

confidence: 99%

“…According to Ho et al (1998), a MS based medium (Murashige & Skoog, 1962) supplemented with naphthalenacetic acid (NAA) (16.2 µmol L -1 ) and 6-benzylaminopurine (BAP) (4.44 µmol L -1 ) can be used for induction of callus and bud regeneration in E. camaldulensis. Mullins et al (1997) also succeeded in regenerating E. camaldulensis buds from leaf explants of in vitro-cultured plants.…”

Section: Introductionmentioning

confidence: 99%

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Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis

Dibax¹,

Eisfeld

Cuquel³

et al. 2005

Sci. agric. (Piracicaba, Braz.)

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Breeding methods based on genetic transformation techniques need to be implemented for Eucalyptus camaldulensis to shorten the long breeding cycles and avoid manipulation of adult trees; that requires the development of plant regeneration protocols enabling development of plants from transformed tissues. The present work aimed to optimise the regeneration process already established for the species. Cotyledonary leaves of E. camaldulensis were cultured in MS medium supplemented with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) combinations. The most efficient treatment for bud indirect regeneration (2.7 µmol L -1 NAA and 4.44 µmol L -1 BAP) was used for further experiments. When explants were kept in the dark during the first 30 days, the percentage of explants forming calluses increased and explant necrosis was reduced in comparison with light-cultured explants. Mineral medium modifications were compared and half-strength MS mineral medium turned out to be as efficient as full-strength medium, producing 54% and 47% of explants with buds, respectively. For shoot elongation, MS medium with halfstrength nitrate and ammonium salts, and 0.2% activated charcoal yielded rooted shoots 1 to 8 cm high after one month. The procedure is an efficient protocol for E. camadulensis plant regeneration, reducing the stages necessary for the obtention of complete plants. Key words: in vitro culture, forest species, culture media, organogenesis REGENERAÇÃO DE PLANTAS DE Eucalyptus camaldulensis A PARTIR DAS EXPLANTES COTILEDONARESRESUMO: A implementação, para espécies florestais, de técnicas de melhoramento baseadas em métodos de transformação genética, permitirá reduzir os longos ciclos de melhoramento e evitar a manipulação de árvores adultas. Isto implica dispor de um protocolo de regeneração que permita o desenvolvimento de plantas a partir de tecidos transformados. Este trabalho teve como objetivo otimizar este protocolo de regeneração para Eucalyptus camaldulensis. Folhas cotiledonares foram cultivadas em meio de cultura MS suplementado com combinações de ácido naftalenoacético (ANA) e 6-benzilaminopurina (BAP). O tratamento mais eficiente em termos de regeneração indireta de gemas foi 2,7 µmol L -1 de ANA combinado com 4,44 µmol L -1 de BAP, o qual foi utilizado nos experimentos posteriores. A manutenção dos explantes no escuro durante os trinta primeiros dias elevou a porcentagem de explantes com calos e reduziu a morte dos explantes, em comparação com os que permaneceram na luz. Modificações da composição mineral do meio MS foram comparadas e mostraram que a redução de metade dos sais foi tão eficiente para a formação de gemas (54% dos explantes) quanto o meio completo (47%). O meio de cultura com a concentração de íons nitrato e amônio reduzida à metade e 0,2% de carvão ativado apresentou-se adequado para o alongamento e enraizamento das brotações que atingiram uma altura de 1 a 8 cm depois de 30 dias. O processo completo representa um protocolo eficiente para a regeneração de plantas de Eucalyptu...

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Section: Effect Of the Combinations Of Naa And Bap On Callogenesis Ancontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis

Dibax¹,

Eisfeld

Cuquel³

et al. 2005

Sci. agric. (Piracicaba, Braz.)

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“…However, SP medium [108,[110][111][112], WPM minerals [113], WPM [114], B5 medium [115][116][117], N7 medium [86,118], JADS medium [98], EDM [119], and MS medium with White vitamins [89] have also been used. Callus is often induced in darkness, although basal organogenesis on shoots can occur in eucalypt shoot cultures that are maintained under light [45,46,64,98,120].…”

Section: Organogenesismentioning

confidence: 99%

“…Mineral adjustments have included the use of MS salts with 2.74 mM NaH 2 PO 4 , or a reduction in MS-medium strength to 1/10, for C. citriodora shoots [69,147]. They have also included the use of MS medium with half-strength macronutrients, or half-strength nitrates, for E. camaldulensis shoots [116,143], or the use of MS macronutrients with half-strength micronutrients for E. grandis × E. urophylla shoots [148]. More often, MS medium is simply reduced to half-strength during root induction, including for shoots of C. citriodora…”

Section: Adventitious Root Formationmentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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“…This is due to its quick growth, short period before harvesting and its use in up to three successive rotations (Ho et al, 1998). The hybrid from E. grandis × E. urophylla has been largely used, especially in Brazil, due to its wood properties, rooting ability and resistance to tumor induced by Cryphonectria cubensis, which result in high levels of productivity and performance for paper, cellulose and fiber industries (Bracelpa, 2007).…”

Section: Introductionmentioning

confidence: 99%

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla

Alcantara

Filho

Quoirin

2011

Sci. agric. (Piracicaba, Braz.)

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The hybrid Eucalyptus grandis × Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two clones tested were observed. The explant most recommended for organogenesis is the basal section of the leaf collected from shoot clusters subcultured every 17 days. Moreover, the leaf explants must be transferred to a fresh bud induction medium every five days. This study also aimed at evaluating factors affecting the genetic transformation of leaf explants with the uidA gene, via co-cultivation with Agrobacterium tumefaciens, such as the pre-culture of the explants on a specific medium, the duration of their co-culture with the bacteria and the addition of acetosyringone to the culture media. The best conditions for the expression of the uidA gene were two days of pre-culture of the leaf tissues, three days of co-culture with the bacteria and the addition of acetosyringone in pre-and co-culture media. Key words: Agrobacterium tumefaciens, micropropagation, tissue culture, transgenic plant, woody species Organogênese e transformação genética transiente do híbridoEucalyptus grandis × Eucalyptus urophylla RESUMO: O híbrido Eucalyptus grandis × Eucalyptus urophylla apresenta alta produtividade e potencial para indústrias de papel, celulose e fibras. A organogênese de explantes foliares de dois clones de E. grandis × E. urophylla foi estudada para verificar fatores como tempo de repicagem das plântulas matrizes em meio de multiplicação; tipo de explantes, folhas inteiras e de meias-folhas (porções basais e apicais) e dos dias que os explantes foliares permaneceram em meio de regeneração. Foram observadas diferenças na capacidade organogênica dos dois clones testados. A parte basal das folhas, coletadas de brotações repicadas a cada 17 dias, apresentou superioridade organogênica. Explantes foliares devem ser transferidos para novo meio de indução de brotações a cada cinco dias. Este estudo também teve como objetivo avaliar fatores que afetam a transformação genética de explantes foliares com o gene uidA, via co-cultivo com Agrobacterium tumefaciens, tais como a pré-cultura, a duração da co-cultura e a adição da acetoseringona no meio de cultura. As melhores condições para a expressão do gene uidA foram a cada dois dias de pré-cultura de explantes foliares, três dias de co-cultura com a bactéria e a adição de acetoseringona nos meios de pré e co-cultura.

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Agrobacterium tumefaciens -mediated transformation of Eucalyptus camaldulensis and production of transgenic plants

Cited by 79 publications

References 16 publications

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis

Tissue Culture of Corymbia and Eucalyptus

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla

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