Tissue Culture of Corymbia and Eucalyptus

Abstract

Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

“…According to Trueman et al (2018), for the success of micropropagation it is necessary that only some explants emit shoots free of contamination, because the beginning of in vitro propagation is the main limiting phase. However, when a large amount of micropropagated material is required, higher rates of bud explants may be required to rapidly increase the amount of material produced.…”

“…Micropropagation through proliferation of axillary buds has been recommended for rejuvenation of selected clones in a forest area, mainly aiming at improving the production process of cloning. Success of a micropropagation protocol depends on the phase of in vitro introduction, given that the subsequent stages of multiplication, lengthening, and rooting can only be carried out after the establishment of aseptic cultures and with vegetative vigor (George et al, 2008;Trueman et al, 2018).…”

MIXOTROPHISM EFFECT ON IN VITRO ELONGATION AND ADVENTITIOUS ROOTING OF Eucalyptus dunnii

“…L -1 BAP (6-benzylaminopurine -Sigma Co.), and 0.5 mg . L -1 of indole-3-butyric acid (IBA) (Sigma ® ) (Trueman et al, 2018).…”

“…However, specie rooting is a challenge in clonal propagation, particularly when adult material is involved. The rejuvenation of propagules has allowed advances on in vitro elongation and adventitious rooting phases (Trueman et al, 2018). In the search for alternatives to rooting improvement in the cloning production process, micropropagation via axillary buds proliferation has been recommended (Xavier et al, 2013).…”

“…Among micropropagation stages, in vitro elongation is primordial in obtaining sprouts for microcutting rooting, which has been performed as much under ex vitro (Xavier et al, 2013) or in vitro (Trueman et al, 2018) conditions. However, research for improvements in the in vitro elongation phase has been intensified, aiming to obtain microstumps.…”