MIXOTROPHISM EFFECT ON IN VITRO ELONGATION AND ADVENTITIOUS ROOTING OF Eucalyptus dunnii

Souza, Denys Matheus Santana Costa; Fernandes, Sérgio Bruno; Avelar, Maria Lopes Martins; Frade, Samira Rangel do Prado; Molinari, Letícia Vaz; Gonçalves, Douglas Santos; Brondani, Gilvano Ebling

doi:10.1590/01047760201925042638

Cited by 14 publications

(12 citation statements)

References 34 publications

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“…In contrast, the use of white spectral quality resulted in greater oxidation in explants of Corymbia torelliana × C. citriodora (Souza et al 2018). Tissue oxidation is a problem with the micropropagation of woody species, as reported in other studies (Oliveira et al 2016;Souza et al 2019;Souza et al 2020b;Faria et al 2021;Faria et al 2022;Souza et al 2022). These results may be linked to internal environmental factors that affect the vigor of explants, where smaller flasks tend to have reduced carbon dioxide concentrations and high ethylene concentrations, and may also be affected by light irradiation, air temperature, and relative humidity (Tisarum et al 2018;Chen et al 2019;Souza et al 2021).…”

Section: Effect Of Spectral Quality On In Vitro Multiplicationmentioning

confidence: 78%

“…Eucalyptus grandis × E. urophylla clone showed the best results of adventitious rooting (Figures 9A, 9C, and 9E). Adventitious rooting of Eucalyptus dunnii is a challenge in clonal propagation, varying from 3.0 to 46.5% (Brondani et al 2011;Oberschelp et al 2015;Souza et al 2019). In contrast, the Eucalyptus grandis × E. urophylla has shown 80.0 to 100.0% (Gallo et al 2017;Souza et al 2020b;Miranda et al 2020).…”

Section: Effect Of Spectral Quality On In Vitro Adventitious Rootingmentioning

confidence: 99%

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Spectral quality influence on in vitro morphophysiological responses of Eucalyptus dunnii Maiden and Eucalyptus grandis W.Hill ex Maiden × E. urophylla S.T.Blake

Frade

Souza

Fernandes

et al. 2023

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Background: In vitro growth and development of plants in the micropropagation stages are influenced by several factors, including the light spectral quality, which has shown important effects on the photomorphogenesis. The work aimed to evaluate the photomorphogenic effect of spectral qualities on in vitro culture of Eucalyptus dunnii and Eucalyptus grandis × E. urophylla. Methods: Six light spectral qualities (i.e., red, white, blue, yellow, purple, and green) on in vitro multiplication, elongation, and adventitious rooting stages were evaluated through analysis of variance followed by a Tukey’s test. Results: White spectral quality was most adequate for in vitro multiplication of Eucalyptus dunnii and Eucalyptus grandis × E. urophylla, as it resulted in less tissue oxidation, longer shoot length, and more buds per explant. Red, blue and yellow spectral qualities increased the chlorophyll a, chlorophyll b, and total chlorophyll (a+b) leaf contents of Eucalyptus dunnii. To promote in vitro elongation, white spectral quality was most suitable for Eucalyptus dunnii, and yellow for Eucalyptus grandis × E. urophylla, as these resulted in more shoot length and shoots per explant. Red, white, blue and purple spectral qualities increased the stomatal density of Eucalyptus dunnii; while the white and yellow were the better for Eucalyptus grandis × E. urophylla. To promote in vitro rooting, the white and yellow spectral qualities caused the best results for the Eucalyptus dunnii and Eucalyptus grandis × E. urophylla, with longer root length and more roots per explant. Eucalyptus dunnii showed reduced adventitious rooting, regardless of spectral quality. Conclusions: Light quality influence the morphophysiological responses of Eucalyptus in different stages of in vitro culture. Our results contribute to maximise the in vitro cloning of important eucalypts species.

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Section: Effect Of Spectral Quality On In Vitro Multiplicationmentioning

confidence: 78%

Section: Effect Of Spectral Quality On In Vitro Adventitious Rootingmentioning

confidence: 99%

Spectral quality influence on in vitro morphophysiological responses of Eucalyptus dunnii Maiden and Eucalyptus grandis W.Hill ex Maiden × E. urophylla S.T.Blake

Frade

Souza

Fernandes

et al. 2023

NZJFS

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“…The efficiency of in vitro organogenesis depends primarily on the source of the explant, on the components of the culture medium and on the environmental conditions (Hesami andDaneshvar 2018, Silva et al 2019). Another factor influencing the organogenic responses in Eucalyptus is the endogenous and exogenous hormonal balance between the cytokinin and auxin present in the plant tissue, which serve as inducing agents for in vitro morphogenesis (Silva et al 2019, Souza et al 2019. Many studies report a favorable effect of TDZ at different stages of callus formation (Jafari et al 2017), with TDZ concentrations below 2.5 mg L -1 , being recommended for effective applications to the tissue of woody species, since high concentrations of this plant growth regulator may limit the indirect organogenesis by reducing the induction of buds and increasing hyperhydricity (Hesami and Daneshvar 2018).…”

Section: Naa (Mg L -1 )mentioning

confidence: 99%

“…Thus, the influence of the incubation conditions has been studied in several Eucalyptus species (Fernando et al 2016) during organogenesis. These changes may occur due to the composition of the culture medium, nutrition, balance between the plant growth regulators, type and ontogenetic age of the explant and determination of the tissues (Brondani et al 2012, Mittal and Sharma 2017, Souza et al 2019.…”

Section: Introductionmentioning

confidence: 99%

Microplants production of Eucalyptus cloeziana from indirect organogenesis

et al. 2020

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Few studies have focused on the in vitro multiplication methods for Eucalyptus cloeziana. In this work we developed an indirect organogenesis protocol to obtain micropropagated plants. The interactions of plant growth regulators in juvenile tissues were evaluated. Hypocotyl, cotyledon and root tissues obtained from in vitro germinated seedlings were subjected to combinations of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D). In vitro callogenesis, adventitious bud induction, shoot elongation, and ex vitro survival and rooting were evaluated. Callogenic structures were observed in all evaluated tissues. Morphogenic characteristics related to the meristematic center was observed. The tissues that presented the callus mass were subcultured in a regeneration culture medium supplemented with 1.0 mg L-1 of 6-benzylaminopurine (BAP). Only hypocotyl and cotyledon induced adventitious bud and shoot elongation. Hypocotyl subjected to 2.0 mg L-1 TDZ had the highest number of elongated shoots. The two tissues (i.e., hypocotyl and cotyledon) combined with thidiazuron were characterized by the ex vitro survival of microcuttings and by adventitious rooting. Results confirmed tissue culture efficiency for microplants production by indirect organogenesis from hypocotyl and cotyledon cultivated with TDZ, and its implementation can be an alternative for forest tree breeding programs of E. cloeziana.

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“…Several propagation techniques have been adopted to promote tissue rejuvenation and/or juvenile shoot induction in adult plants, such as girdling (Hartmann et al, 2011), cutting (Trueman et al, 2013), mini-cutting (Brondani et al, 2012a), coppicing (Xavier et al, 2013), epicormic shoot induction (Wendling et al, 2013) and micropropagation (Brondani et al, 2012b;Brondani et al, 2018;Souza et al, 2019a). These techniques can be used to rescue and clone selected trees (Baccarin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

In vitro ESTABLISHMENT OF Eucalyptus AND Corymbia SPECIES FROM EPICORMIC SHOOTS

et al. 2020

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The importance of Eucalyptus and Corymbia has been evident in forestry programs, mainly due to their adaptation to various environmental conditions and the multiple products that can be obtained. As the selection and cloning of superior individuals are usually performed in adulthood, the development and adaptation of the vegetative propagation techniques that enable the rescue and in vitro establishment of species are necessary. Thus, the present study aimed to induce epicormic shoots from pruned branches of adult trees and to promote the in vitro establishment of nine species: six of Eucalyptus and three of Corymbia. The material used to obtain the explants came from the selection of two 44-year-old mother plants of each species, from species, and provenance tests. The number of buds and shoots of each branch was evaluated at 15, 25, 35, and 45 days after the beginning of the experiment in a greenhouse. According to the results, the induction of epicormic shoots from pruned branches was considered a viable and efficient technique to obtain propagule sources from adult mother plants of all species, presenting a gradual increase in the evaluated characteristics over time. Also, in vitro culture is an effective alternative to improve the propagation of the evaluated species, with an establishment of up to 62.5%. However, for Corymbia citriodora (Hook.) K.D. Hill & L.A.S Johnson and Eucalyptus tereticornis Smith it is necessary to develop further studies to enable the in vitro establishment.

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MIXOTROPHISM EFFECT ON IN VITRO ELONGATION AND ADVENTITIOUS ROOTING OF Eucalyptus dunnii

Abstract: Different types of sealing and sucrose concentrations influence in vitro elongation and adventitious rooting. Higher gas exchange (CO 2) favors the in vitro plant growth. The autotrophic system for the in vitro cultivation of Eucalyptus dunnii was not efficient.

Cited by 14 publications

References 34 publications

Spectral quality influence on in vitro morphophysiological responses of Eucalyptus dunnii Maiden and Eucalyptus grandis W.Hill ex Maiden × E. urophylla S.T.Blake

Spectral quality influence on in vitro morphophysiological responses of Eucalyptus dunnii Maiden and Eucalyptus grandis W.Hill ex Maiden × E. urophylla S.T.Blake

Microplants production of Eucalyptus cloeziana from indirect organogenesis

In vitro ESTABLISHMENT OF Eucalyptus AND Corymbia SPECIES FROM EPICORMIC SHOOTS

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