Management of Bamboo Genetic Resources and Clonal Production Systems

Konzen, Enéas Ricardo; Souza, Denys Matheus Santana Costa; Fernandes, Sérgio Bruno; Brondani, Gilvano Ebling; Carvalho, Dulcinéia de; Campos, Wellington Ferreira

doi:10.1007/978-981-16-1310-4_9

Cited by 2 publications

(9 citation statements)

References 68 publications

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“…The maintenance of the stock plants in a protected environment contributes to less exposure to environmental variations and consequently the obtention of more vigorous shoots with lower levels of contamination. In addition, because the distribution of stock plants in the greenhouse there was a reduction in fungal contamination with greater distance between plant root systems, due to less mobility of microorganisms in tissues (Sandhu et al, 2018;Torres et al, 2019;Konzen et al, 2021a). It is important to highlight that the greatest source of fungal contamination through endophytic fungal occurs in the root tissues in bamboos (Torres et al, 2019).…”

Section: In Vitro Establishmentmentioning

confidence: 99%

“…The formation of clonal micro-garden in a semihydroponic system is widely used for several forest species (Brondani et al, 2012;Brondani et al, 2018;Araújo et al, 2019;Konzen et al, 2021a) and allows greater micropropagation efficiency. However, for bamboo, it is not so widespread.…”

Section: Survival Ex Vitro Rooting and Clonal Micro-gardenmentioning

confidence: 99%

“…The production of clonal bamboo plants needs technological advances to effectively contribute to the encouragement of their cultivation aimed at the production of sustainable biomass. The bamboo species presents variations in seed production (Ramanayake et al, 2001;Vengala et al, 2008), a fact that limits the production of plants by seeds, and thus, vegetative propagation is a significant alternative for plant multiplication (Furlan et al, 2018;Ribeiro et al, 2016;Ribeiro et al, 2020;Konzen et al, 2021a). Although frequent, bamboo vegetative propagation by traditional methods, such as the breaking up of clumps, still cannot meet the growing demands for seedlings and the expectations generated with the increase in product diversification (Akinlabi et al, 2017;Rajput et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The most frequent ones are endophytic organism contamination, hyperhydricity, oxidation of the culture medium due to the release of phenolic substances, unstable multiplication rate and rooting difficulty (Ray and Ali, 2017;Sandhu et al, 2018). For minimising such challenges, researchers have conducted studies that test new asepsis techniques, new culture medium, exogenous applications of plant growth regulators and factors that promote events such as shoot induction and adventitious rooting (Furlan et al, 2018;Sandhu et al, 2018;Lin et al, 2019;Ribeiro et al, 2020;Silveira et al, 2020;Konzen et al, 2021a).…”

Section: Introductionmentioning

confidence: 99%

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Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Teixeira

Gonçalves

Modesto

et al. 2021

CERNE

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Background:We have developed a micropropagation technique methodology to clonal microgarden formation of Bambusa vulgaris selected adult plant. Collection site (i.e., stock plant cultivated in different environments) and seasonality of shoot collection (i.e., months of the year) effects on in vitro culture were evaluated. Explants that showed normal development were ex vitro rooted in miniincubator system, and three culture media (WPM, MS and deionized water + agar) supplemented with plant growth regulators (IBA, NAA and BAP) and antibiotic (streptomycin sulphate and culture medium free -control) were evaluated. Micropropagated plants were acclimatized in a shadow house and transferred to a semi-hydroponic system for establishment of a clonal micro-garden. The efficacy of the cloning protocol was determined with genetic fidelity analysis by ISSR molecular markers.Results: Considering all inoculations, 21.9% of nodal segments were in vitro established in nine shoot collections. The rooting percentage was 36.6%, and no interactions were observed between the use of culture medium and antibiotic. Culture medium free antibiotic resulted in 80.0% of survival and 50.0% of adventitious rooting. Micropropagated plants presented adequate growth and adaptation to ex vitro conditions in the clonal micro-garden. Molecular analyses by ISSR markers indicated the absence of genetic variations, and histological analyses revealed normal adventitious root formation originating from meristematic cells. Conclusion:The formation of a clonal micro-garden was demonstrated, proving the feasibility of the tested technique. Our results contribute to the development of a clonal propagation protocol for B. vulgaris selected adult plants.

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Section: In Vitro Establishmentmentioning

confidence: 99%

Section: Survival Ex Vitro Rooting and Clonal Micro-gardenmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Teixeira

Gonçalves

Modesto

et al. 2021

CERNE

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“…It is suitable for the production of cellulose and papermaking; the manufacture of household utensils and furniture; as food -for its edible sprouts; fiber; biofuel; for construction and the manufacture of musical instruments (INBAR, 2015;SINGH et al, 2012). Homogeneous plantings of the species can provide a rapid source of renewable biomass (MUSTAFA et al, 2021;KONZEN et al, 2021a), and the cloning of selected plants on an industrial scale (e.g., in biofactories) can provide plants in desirable quality and quantity.…”

Section: Introductionmentioning

confidence: 99%

CLONAL MICROPLANT PRODUCTION, MORPHOLOGICAL EVALUATION AND GENETIC STABILITY OF Dendrocalamus asper (Schult. & Schult.) Backer ex. K. Heyneke

Gonçalves

Souza

Molinari

et al. 2023

NAT

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Bamboo species have many commercial applications, considering that homogeneous plantations (formed from clonal plants) are essential to high sustainable biomass production. The cloning of selected plants on an industrial scale through in vitro cultivation has many advantages, being important for the supply of plants in sufficient quantity and quality to meet commercial demand. The control of the cloning is the basis for an industrial scale, and its knowledge can optimize the process. This work aimed to evaluate the cloning of Dendrocalamus asper selected plant through micropropagation. Morphological features by scanning electron microscopy and genetic stability with ISSR molecular markers were evaluated. Four times of immersion in sodium hypochlorite (NaOCl) on in vitro establishment of nodal segments were evaluated. The established explants were transferred to a culture medium that was supplemented with three concentrations of 6-benzylaminopurine (BAP). Three concentrations of indole-3-butyric acid (IBA) to the in vitro adventitious rooting were evaluated. NaOCl application for 10 min resulted in 71.4 % of establishment in 30 d. Supplementation of the culture medium with 2.0 and 3.0 mg L-1 BAP de resulted in the highest averages for multiplication and elongation stages. The formation of adventitious roots occurred with 4.0 mg L-1 IBA of supplementation. Micropropagated plants showed normal morphological features and genetic stability, confirming the cloning of selected plant. Keywords: bamboo; micropropagation; vegetative propagation; In vitro culture; ISSR; plant growth regulator. Produção de microplantas clonais, avaliação morfofisiológica e estabilidade genética de Dendrocalamus asper (Schult. & Schult.) Backer ex. K. Heyneke RESUMO: Espécies de bambus apresentam diversas aplicações comerciais, visto que os plantios homogêneos (formados a partir de plantas clonais) são essenciais para a alta produção de biomassa sustentável. A clonagem de plantas selecionadas em escala industrial por meio do cultivo in vitro apresenta muitas vantagens, sendo uma importante ferramenta para o fornecimento de plantas em quantidade e qualidade suficientes para atender a demanda comercial. O controle da clonagem é a base para escala industrial, e seu conhecimento pode otimizar os processos. O trabalho teve como objetivo avaliar a clonagem de planta selecionada de Dendrocalamus asper por meio da técnica de micropropagação. Foram avaliadas as características morfológicas por microscopia eletrônica de varredura e estabilidade genética por meio de marcadores moleculares ISSR. Além disso, foram avaliados quatro tempos de imersão em hipoclorito de sódio (NaOCl) no estabelecimento in vitro de segmentos nodais. Os explantes estabelecidos foram transferidos para um meio de cultura que foi suplementado com três concentrações de benzilaminopurina (BAP). Por fim, foram avaliadas três concentrações de ácido indolbutírico (AIB) durante o enraizamento adventício in vitro. A adição de NaOCl por 10 min resultou em 71,4 % de estabelecimento em 30 d. A suplementação do meio de cultura com 2,0 e 3,0 mg L-1 BAP resultou nas maiores médias para as fases de multiplicação e alongamento. A formação de raízes adventícias ocorreu com a suplementação de 4,0 mg L-1 de AIB. Plantas micropropagadas apresentaram características morfológicas normais e estabilidade genética, confirmando a clonagem da planta selecionada. Palavras-chave: bambu; micropropagação; propagação vegetativa; cultivo in vitro; ISSR; regulador de crescimento vegetal.

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Management of Bamboo Genetic Resources and Clonal Production Systems

Cited by 2 publications

References 68 publications

Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

Clonal micro-garden formation of Bambusa vulgaris: effect of seasonality, culture environment, antibiotic and plant growth regulator on in vitro culture

CLONAL MICROPLANT PRODUCTION, MORPHOLOGICAL EVALUATION AND GENETIC STABILITY OF Dendrocalamus asper (Schult. & Schult.) Backer ex. K. Heyneke

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