Age-related Changes in Glutathione Concentration, Glutathione Peroxidase, Glutathione-S-Transferase, and Superoxide Dismutase Activities in Senescence Accelerated Mice

Induction after Administering Paraquat of Heme Oxygenase-1 and Heat Shock Protein 70 in the Liver of Senescence-accelerated Mice

“…Of these factors, an accumulation of molecular oxidative damage is attributed to the senescenceassociated loss of functional capacity.4) In our laboratory, an age-associated decline in the functional defense capacity against oxidants was shown with senescence-accelerated mice (SAMs) 5 -~ 7) which has been widely employed for investigating the mechanism of the aging process: 1) the activity of such antioxidative enzyme activities as glutathione peroxidase, glutathionc-S-transferase and superoxide dismutase in erythrocytes and liver decreased with age,8) 2) the ratio of DNA single-strand breaks and the level of oxidized protein in the liver increased with age, 9 11) and 3) these changes in mice that were prone to accelerated senescence (SAMPI tFky) were more marked than in mice that were resistant to accelerated senescence (SAMR 1/ Fky). [8][9][10][11] The ability to withstand stressors such as oxidizing molecules may be the key requirement for a longer life span. The HSP70 family of proteins are expressed in most organisms with evolutional conservation, 12, 13) the HSP70 family including hsp70 and hsc70.…”