The SAM Strain of Mice, a Higher Oxidative Stress, Age-Dependent Degenerative Disease, and Senescence Acceleration Model

Chiba, Yoichi; Shimada, Atsuyoshi; Hosokawa, Masanori

doi:10.1007/978-1-60761-602-3_18

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“…We have recently reported a spontaneous animal model for photoaging, probably caused by an exaggerated intrinsic mechanism [21]. Accelerated senescence-prone (SAMP) mice show an accelerated senescence process, shorter lifespan, higher oxidative status, and an earlier onset and more rapid progression of age-associated pathological phenotypes as compared to accelerated senescence-resistant (SAMR) mice, a closely related control strain which shows a normal aging process [22]- [24]. SAMP1 mice, a strain of the SAMP mice, exhibited histopathological and gene expression changes similar to those in human photoaged skin with advancing age, even in the absence of UV irradiation.…”

Section: Introductionmentioning

confidence: 99%

Differences in the Histopathology and Cytokine Expression Pattern between Chronological Aging and Photoaging of Hairless Mice Skin

Sakura¹,

Chiba²,

Kamiya³

et al. 2014

MRI

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Skin photoaging is a complex, multifactorial process resulting in functional and structural changes of the skin, and different phenotypes from chronological skin aging are well-recognized. Ultraviolet (UV)-irradiated hairless mice have been used as a skin photoaging animal model. However, differences in morphology and gene expression patterns between UV-induced and chronological skin changes in this mouse model have not been fully elucidated. Here we investigated differences in histopathology and cytokine expression between UV-irradiated and non-irradiated aged hairless mice to clarify the factor(s) that differentiate photoaging from chronological skin aging phenotypes. Eight-week-old HR-1 hairless mice were divided into UV-irradiated (UV-irradiated mice) and non-irradiated (control mice) groups. Irradiation was performed three times per week for 10 weeks. In addition, 30-week-old HR-1 hairless mice were reared until 70 weeks of age without UV irradiation (aged mice). Histopathologies revealed that the flattening of dermal-epidermal junctions and epidermal thickening were observed only in UV-irradiated mice. Decreases in fine elastic fibers just beneath the epidermis, the thickening of elastic fibers in the reticular dermis, and the accumulation of glycosaminoglycans were more prominent in UV-irradiated mice as compared to non-irradiated aged mice. Quantitative PCR analyses revealed that UV-irradiated mice showed * Corresponding author. M. Sakura et al. 83 an increase in the expression of IFN-γ. In contrast, aged mice exhibited proportional up-regulation of both pro-inflammatory and anti-inflammatory cytokines. The IFN-γ/IL-4 ratio, an indicator for the balance of pro-inflammatory and anti-inflammatory cytokines, was significantly higher in UVirradiated mice as compared to control and non-irradiated aged mice. An elevated IFN-γ/IL-4 ratio was also observed in aged senescence-accelerated mouse-prone 1 (SAMP1) mice, a spontaneous skin photoaging model we recently reported. Thus, an imbalance between pro-inflammatory and anti-inflammatory cytokines might be a key factor to differentiate photoaged skin from chronologically-aged skin.

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Section: Introductionmentioning

confidence: 99%