Different molecular species of cardiolipin were prepared from tissue of rats fed diets containing different oils. The effects of these different species of cardiolipins on bovine heart cytochrome c oxidase activity were examined using reconstituted vesicles. Cytochrome c oxidase was stimulated effectively by cardiolipin molecular species rich in linoleoyl/linoleoyl species.
ra ed (NIR) transflectance spectroscopy scanning from 1100 nm to 2500 nm was applied for the Near-inf r determination of palmitic acid (C16=0), oleic acid (C18=1) and linoleic acid (C18=2), the major fatty acids in edible oils.Edible oil samples were divided into two sets: 95 and 46 samples as a calibration and a prediction set, respectively.The multiple regression equations (MRES) established for the calibration set between the reference data analyzed by gas chromatography (GC) and the NIR raw spectral data gave the multiple correlation coefficients of 0.996, O. 989 and 0.993 for C16=0. C18=1 and C18=2, respectively. The best MRES Were established with the NIR raw spectral data recorded at 1724, 1756, 2140 and 2180 nm. The Fourier transform mid-infrared spectra of the standard C16=0, C18=1 and C18=2 supported the belief that the absorptions at these wavelengths were attributed to the CH, of the straight carbon chain and the C=C of the unsaturated fatty acid. Standard errors of predictions between the data calculated froun the best MRES and the reference data analyzed by GC for the prediction samples were 0.925%, 2.186% and 2.011%, and the correlation coefficients between those were larger than 0.99 for C16=(~ C18=1 and C18=2, respectively. The obtained results indicate that the NIR procedure can potentially be used as a nondestructive analysis method for the purpose of rapid and sunple determmatron of C160 C18 1 and C182 m edible oils
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