Age-Related Aspects of the Involvement of Heat Shock Proteins in the Pathogenesis of Osteoarthritis

Кабалык, М. А.

doi:10.1134/s2079057017040063

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…The pathogenesis of OA is multifactorial and extremely complex due to a great number of mollecular and cellular mechanisms involved. Essential contributors to articular remodeling have been identified in recent decades including heat shock proteins, matrix metalloproteinases, growth factors and cell differentiation [6]. Pathological articular angioproliferation due to VEGF expression is known to contribute to OA initiation and progression [7].…”

Section: Discussionmentioning

confidence: 99%

Molecular and cellular effects of intraarticular injection of betamethasone in experimental osteoarthritis

Кабалык¹,

Невзорова²,

Dubov³

et al. 2020

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The objective of the study was to assess articular cartilage (AC) and subchondral bone (SCB) remodeling, expression of matrix metalloproteinase 9 (MMP-9) in tissues, vascular endothelial growth factor (VEGF) in experimental osteoarthritis (OA). Material and methods Experimental comparative study was conducted on 12 outbred guinea pigs of both sexes aged 28-30 weeks that were divided into 2 groups of 6 animals each. An injury to the knee joints of hind limbs of control and experimental animals was mechanically simulated by closed scarification using a sterile needle. No treatment was provided for controls. Experimental animals were given an intraarticular injection of betamethasone (BMZ) of 0.1 mg/kg every two weeks after two weeks of injury. Two subjects of each group were euthanized at 30, 45, 60 days and knee samples collected. Immunohistochemical expression of VEGF and MMP-9 was determined in tissues. Results A statistically significant decrease in VEGF positive chondrocytes and precipitate density, an increase in positive chondrocytes and intensity of tissue response to MMP-9 as compared to those in controls was observed in BMZ animals at 30 days of experiment. A statistically significant decrease in VEGF positive cells and precipitate density, an increase in VEGF positive chondrocytes as compared to those in controls were observed in BMZ animals at 45 days of experiment. Significant reduction in VEGF positive cells and deposit density, significantly higher density of MMP-9 positive precipitates as compared to those in controls were noted at 60 days of BMZ injections. Conclusion Intraarticular injections of BMZ demonstrated a negative effect on AC and SCB with articular tissue remodeling initiated through activation mechanisms of extracellular matrix degradation, as evidenced by high expression of MMP-9. BMZ was shown to block pathological angiogenesis via VEGF inhibition.

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Section: Discussionmentioning

confidence: 99%

Molecular and cellular effects of intraarticular injection of betamethasone in experimental osteoarthritis

Кабалык¹,

Невзорова²,

Dubov³

et al. 2020

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“…Как уже сообщалось, смешанный эндотип реализуется через патогенетические механизмы ангиопролиферации, эндотелиальной дисфункции и апоптоза. Также известно, что хондроитина сульфат положительно влияет на функцию эндотелия, замедляет процессы атерогенеза [26,27]. Глюкозамин обладает регулирующим влиянием на аутофагию и запрограммированную клеточную смерть путем регуляции сигнального каскада m-TOR [28,29].…”

Section: Discussionunclassified

Effectiveness of drugs with delayed-release structurally modified action depending on the phenotype of osteoarthritis

Кабалык

2020

Medicinskij sovet

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Aim. To evaluate the effectiveness of a fixed combination of honadroitin and glucosamine sulfate (Teraflex, Bayer) in the treatment of osteoarthritis (OA), depending on the molecular phenotype of the disease.Materials and methods. A 6-month prospective, open, randomized trial included 65 patients with OA of the knee joints who were prescribed therapy with Teraflex (Bayer) daily dose of 1500 mg + 1200 mg. Kinetic assessment of articular status was performed using a visual analogue pain scale and a WOMAC questionnaire, and serum concentrations of CRTAP (cartilage-associated protein), OSGIN-1 (oxidative stress-induced growth inhibitor 1), IL-1β (interleukin-1 beta) were determined in blood serum. Measurements of these parameters were made at the beginning of the study, after 3 and 6 months.Results. It was established that the rate of onset of the therapeutic effect and the effect on the molecular patterns of inflammation and oxidative stress depend on the phenotype of the disease. So, with oxidative and mixed phenotypes of the disease, clinical efficacy is observed in the treatment of teraflex after 3 months from the start of therapy. Indicators of oxidative stress during treatment decreased in the group of patients with the oxidative phenotype of the disease, while the level of interleukin-1 significantly decreased only in groups of patients with inflammatory and mixed OA phenotypes.Conclusions. The results indicate the effectiveness and safety of the drug Teraflex (Bayer) for the treatment of patients with OA. The results of the study indicate the targeted effect of a fixed combination of chondroitin + glucosamine on the molecular mechanisms of the disease.

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Effect of Arterial Hypertension and Hyperlipidemia on the Remodeling of Articular Cartilage and the Development of Osteoarthritis (Experimental Study)

et al. 2020

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Age-Related Aspects of the Involvement of Heat Shock Proteins in the Pathogenesis of Osteoarthritis

Cited by 4 publications

References 18 publications

Molecular and cellular effects of intraarticular injection of betamethasone in experimental osteoarthritis

Molecular and cellular effects of intraarticular injection of betamethasone in experimental osteoarthritis

Effectiveness of drugs with delayed-release structurally modified action depending on the phenotype of osteoarthritis

Effect of Arterial Hypertension and Hyperlipidemia on the Remodeling of Articular Cartilage and the Development of Osteoarthritis (Experimental Study)

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