Effect of Arterial Hypertension and Hyperlipidemia on the Remodeling of Articular Cartilage and the Development of Osteoarthritis (Experimental Study)

Кабалык, М. А.; Kovalenko, T. S.; Невзорова, В. А.; Суханова, Г. И.

doi:10.1134/s2079057020010087

Cited by 4 publications

(6 citation statements)

References 23 publications

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“…IL-10 is known to be a potent anti-inflammatory cytokine, and IL-10 deregulation plays a role in the development of OA. Moreover, existing studies [ 25 , 26 ] have shown that lipid metabolism imbalance contributes to the occurrence and development of OA, and hyperlipidemia is closely related to the immune-inflammatory response of OA patients. APN, an indicator related to lipid metabolism, induces inflammatory responses [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Correlation analysis of differentially expressed long non-coding RNA HOTAIR with PTEN/PI3K/AKT pathway and inflammation in patients with osteoarthritis and the effect of baicalin intervention

et al. 2023

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Objective This study aims to investigate the correlation of long non-coding RNA HOX transcript antisense RNA (lncRNA HOTAIR) with the PTEN/PI3K/AKT pathway and clinical-related indicators in osteoarthritis (OA) and determine the effect of baicalin intervention. Methods The levels of clinical lipid metabolism indexes and immune-inflammatory indexes in OA patients and normal controls was detected. OA chondrocytes (OA-CHs) were induced with peripheral blood mononuclear cells (PBMCs), followed by baicalin treatment (50 ug/mL). RT-qPCR was performed to measure lncRNA HOTAIR expression. The levels of inflammatory cytokines and adiponectin were detected using ELISA kits. CCK-8 assay was used to assess the viability of CHs. The related protein expression was measured using Western blot analysis. Results LncRNA HOTAIR might act as a biomarker of OA in vivo. LncRNA HOTAIR was positively correlated with TC, hs-CRP, IgA, TNF-α, and VAS score. Overexpression of lncRNA HOTAIR in vitro inhibited cell proliferation, reduced IL-10 and PTEN expression, but augmented TNF-α, p-PI3K, and p-AKT proteins in OA-CHs stimulated by OA-PBMCs. The changes of above indexes were also observed in OA-CHs stimulated by OA-PBMCs treated with si-lncRNA HOTAIR or baicalin, implying the synergistic effects of baicalin and lncRNA HOTAIR silencing on OA. Conclusions Conclusively, lncRNA HOTAIR was highly expressed in OA-CHs, which facilitated OA inflammatory responses by orchestrating inflammatory cytokines and the PTEN/PI3K/AKT pathway. Baicalin exerted therapeutic effects by inhibiting the expression of lncRNA HOTAIR, decreasing the protein levels of p-PI3K and p-AKT, and increasing the protein levels of PTEN, APN, and ADIPOR1.

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Section: Discussionmentioning

confidence: 99%

Correlation analysis of differentially expressed long non-coding RNA HOTAIR with PTEN/PI3K/AKT pathway and inflammation in patients with osteoarthritis and the effect of baicalin intervention

et al. 2023

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“…IL-10 level was inhibited in the progression of OA. Existing studies [23,24] have shown that lipid metabolism imbalance plays an important role in the occurrence and development of OA, which is a risk factor for the occurrence of OA and hyperlipidemia and is closely related to the immune-in ammatory response of OA patients. APN, an indicator related to lipid metabolism, induces in ammatory responses [25] .…”

Section: Discussionmentioning

confidence: 99%

Expression of Long Noncoding RNA HOTAIR and Its Influence on the PTEN/PI3K/AKT Pathway and Inflammation in Patients With Osteoarthritis

Chen

Liu

Sun

et al. 2021

Preprint

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Objective: Our research was designed to investigate the correlations among expression of PTEN/PI3K/AKT pathway, clinical-related indicators, and long noncoding RNA HOX transcript antisense RNA (lncRNA HOTAIR) in osteoarthritis (OA). Methods: The expression of immune-inflammatory indicators was detected in OA patients and normal people, and peripheral blood mononuclear cells (PBMCs) were extracted to induce human chondrocytes (CHs). Reverse transcription-quantitative polymerase chain reaction was performed to measure lncRNA HOTAIR expression. The levels of inflammatory cytokines and adiponectin were detected using enzyme-linked immunosorbent assay. Cell Counting Kit-8 was used to assess the viability of CHs. Western blot analysis was utilized to evaluate related protein expression.Results: LncRNA HOTAIR showed high expression in PBMCs of OA patients with the sensitivity and specificity of receiver-operating characteristics (ROC) curve according to the area under the ROC curve, indicating that lncRNA HOTAIR might act as a biomarker of OA. Moreover, lncRNA HOTAIR was positively correlated with total cholesterol, high sensitivity C-reactive protein, immunoglobulin G, tumor necrosis factor-α (TNF-α), and visual analog scale score, which were found to be independent risk factors for lncRNA HOTAIR expression. Besides, overexpression of lncRNA HOTAIR diminished cell viability and IL-10 expression but augmented TNF-α expression in OA-CHs stimulated by OA-PBMCs. Meanwhile, lncRNA HOTAIR overexpression elevated the levels of PI3K, AKT proteins and reduced PTEN protein expression in OA-CHs.Conclusions: Conclusively, lncRNA HOTAIR was upregulated in PBMCs of OA patients, which facilitated the inflammatory response of OA by orchestrating inflammatory cytokines and the PTEN/PI3K/AKT pathway.

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“…Кроме того, Hussain SM, et al (2014г) [13] [23]. В более поздних исследованиях было установлено, что в условиях экспериментальной АГ в СХК развивается ишемия, деструктивное ремоделирование, которые приводят к клеточному стрессу в суставном хряще (СХ) с формированием гипертрофической дифференцировки хондроцитов [24]. Показано также, что в этом процессе главенствующую роль играют эндотелий-зависимые молекулярные механизмы.…”

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“…В экспериментальных исследованиях показано, что процесс метаболизма ХС в хондроцитах регулируется гидролазами, повышенная активность которых приводит к формированию ОА [43]. Также известно, что в условиях экспериментальной гиперлипидемии в тканях суставов запускаются процессы апоптоза, ангиопролиферации и патологической оссификации [24]. Эти процессы регулируются VEGF, CD34+ и ММП [25,44].…”

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“…Например, результаты национального исследования BEACH (Bettering the Evaluation and Care of Health), целью которого было определение компонентов мультиморбидности у 8707 жителей Австралии, показали, что наиболее распространённой коморбидной комбинацией была АГ+ГЛП+ОА [47]. Несмотря на скромное число исследований, посвященных изучению сочетанного влияния кардиометаболических ФР на ткани суставов, имеются данные о том, что сочетание АГ и ГЛП в экспериментальных условиях приводит к более значительным изменениям в суставных тканях по сравнению с изолированным влиянием указанных факторов [24]. При этом в СХК суставов происходит деструктивный процесс, прекращающий поступление кислорода и питательных веществ в СХ, в котором в свою очередь происходят агрессивные процессы деградации и оссификации [44].…”

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Cardiovascular diseases and osteoarthritis: general mechanisms of development, prospects for combined prevention and therapy

Кабалык

Невзорова

2021

Cardiovasc Ther Prev

Self Cite

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Проведен критический анализ результатов исследований по изучению взаимосвязи сердечно-сосудистых заболеваний и остеоартрита, дана оценка возможности их совместной профилактики и лечения. Анализ экспериментальных, клинических и крупномасштабных исследований последних лет позволяет выдвинуть обоснованную концепцию, согласно которой ткани суставов являются органом-мишенью для ряда кардиоваскулярных факторов. Артериальная гипертония, гиперлипидемия, ожирение относятся к наиболее значимым факторам риска развития дегенеративных заболеваний суставов. В ряде исследований установлена первичная роль данных факторов в патогенезе остеоартрита, в связи с чем приведена попытка клинической классификации сердечно-сосудистой коморбидности при остеоартрите. В настоящее время имеются основания полагать, что активная коррекция кардиоваскулярных факторов с использованием нефармакологических и фармакологических подходов может быть использована в лечении остеоартрита.

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Effect of Arterial Hypertension and Hyperlipidemia on the Remodeling of Articular Cartilage and the Development of Osteoarthritis (Experimental Study)

Cited by 4 publications

References 23 publications

Correlation analysis of differentially expressed long non-coding RNA HOTAIR with PTEN/PI3K/AKT pathway and inflammation in patients with osteoarthritis and the effect of baicalin intervention

Correlation analysis of differentially expressed long non-coding RNA HOTAIR with PTEN/PI3K/AKT pathway and inflammation in patients with osteoarthritis and the effect of baicalin intervention

Expression of Long Noncoding RNA HOTAIR and Its Influence on the PTEN/PI3K/AKT Pathway and Inflammation in Patients With Osteoarthritis

Cardiovascular diseases and osteoarthritis: general mechanisms of development, prospects for combined prevention and therapy

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