Age estimation via quantification of signal-joint T cell receptor excision circles in Koreans

Age Estimation in Living Egyptians Using Signal Joint T‐cell Receptor Excision Circle Rearrangement

“…A person's biogeographic ancestry did not influence this approach using sjTREC levels for age estimation. This agerelated DNA maker approach is suitable for age estimation from blood samples among Dutch (15), Chinese (16), and Koreans (25). We found that using sjTREC levels for age estimation is suitable for Egyptians too.…”

“…We suggested that assessment of sjTREC in peripheral blood might be a valuable tool in age estimation. Similarly, Zubakov et al found that sjTREC levels declined in an age‐dependent manner in blood samples with R 2 = 0.835, standard error of the estimate ±8.9 years, Ou et al found that sjTREC levels declined in an age‐dependent manner in blood samples with R 2 = 0.759, and Cho et al declared the linear negative regression curve between sjTREC levels and age with R 2 = 0.807, standard error of the estimate ±8.49 years.…”

Gene analysis of signal-joint T cell receptor excision circles and their relationship to age in dogs

“…The levels of ␦Rec-J␣ sjTRECs in blood can be quantified by real-time quantitative PCR in humans (Hazenberg et al, 2001), and it is largely accepted that ␦Rec-J␣ sjTRECs are surrogate markers for thymic function (Kong et al, 1999;Wysoczanska, 2008). Previous studies have demonstrated an age-related progressive decline in blood ␦Rec-J␣ sjTREC levels (Zubakov et al, 2010;Ou et al, 2011Ou et al, , 2012Cho et al, 2014), possibly echoing age-related thymic atrophy. The measurement of blood ␦Rec-J␣ sjTREC levels and other molecular biology-based methods, including analysis of mitochondrial DNA 4977 bp-fragment deletion (Baumer et al, 1994;Hsieh et al, 1994;Lee et al, 1994;Meissner et al, 1997), telomere DNA fragment shortening (Tsuji et al, 2002;Ren et al, 2009) and methylation levels at specific genomic loci (Akira et al, 1987), have been applied to predict the age of humans for forensic purposes.…”

Analysis of DNA methylation of potential age-related methylation sites in canine peripheral blood leukocytes

“…However, the age of a dog is not always self-evident in situations where breeding is uncontrolled. Several molecular biology-based age-prediction methods have been reported in humans; these include quantitative analysis of T-cell receptor excision circles [4, 19, 21, 32], mitochondrial DNA fragment deletion [1, 2, 11, 15, 20], telomere DNA fragment shortening [24, 28] and age-related DNA methylation levels at specific genomic loci [3, 18, 26, 29, 31]. Although radiography can be used to predict the age of young dogs (less than 1 year old) by their skeletal development [14, 25], the age of adult dogs is usually predicted by subjective observations of characteristics, such as overall appearance or tooth abrasion.…”