Gene analysis of signal-joint T cell receptor excision circles and their relationship to age in dogs

Ito, Genta; Yoshimura, Kuniko; Momoi, Yasuyuki

doi:10.1016/j.vetimm.2015.05.003

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…The same finding was reported in a recent study of a small cohort of mixed breed dogs in Japan [21], and is also apparent in some human studies [31, 35]. The individual variation in sj-TREC values within the Labrador retriever breed might offer opportunities for studying genetic factors that are associated with thymic output.…”

Section: Discussionsupporting

confidence: 81%

“…A recent study has demonstrated that sj-TRECs can be measured in companion animals [21], although no significant correlation between sj-TREC values and age was identified in the small group of mixed breed dogs examined, and the relationship between sj-TREC and thymic size was not investigated. Studies in pedigree dogs have demonstrated that there are breed-related differences in longevity [22, 23], the rate of aging [24, 25], and susceptibility to diseases associated with aging [23, 26].…”

Section: Introductionmentioning

confidence: 99%

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An Age-Associated Decline in Thymic Output Differs in Dog Breeds According to Their Longevity

et al. 2016

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The age associated decline in immune function is preceded in mammals by a reduction in thymic output. Furthermore, there is increasing evidence of a link between immune competence and lifespan. One approach to determining thymic output is to quantify signal joint T cell receptor excision circles (sj-TRECs), a method which has been developed and used in several mammalian species. Life expectancy and the rate of aging vary in dogs depending upon their breed. In this study, we quantified sj-TRECs in blood samples from dogs of selected breeds to determine whether there was a relationship between longevity and thymic output. In Labrador retrievers, a breed with a median expected lifespan of 11 years, there was an age-associated decline in sj-TREC values, with the greatest decline occurring before 5 years of age, but with sj-TREC still detectable in some geriatric animals, over 13 years of age. In large short-lived breeds (Burnese mountain dogs, Great Danes and Dogue de Bordeaux), the decline in sj-TREC values began earlier in life, compared with small long-lived breeds (Jack Russell terriers and Yorkshire terriers), and the presence of animals with undetectable sj-TRECs occurred at a younger age in the short-lived breeds. The study findings suggest that age-associated changes in canine sj-TRECs are related to breed differences in longevity, and this research highlights the use of dogs as a potential model of immunosenescence.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

An Age-Associated Decline in Thymic Output Differs in Dog Breeds According to Their Longevity

et al. 2016

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“…Although radiography can be used to predict the age of young dogs (less than 1 year old) by their skeletal development [14, 25], the age of adult dogs is usually predicted by subjective observations of characteristics, such as overall appearance or tooth abrasion. Previously, we attempted molecular biology-based age-prediction by measuring the level of blood signal joint T-cell receptor excision circles (sjTREC) in dogs [12]; sjTREC levels reflect the number of T-cells freshly recruited from the thymus and thus potentially indicate age-related thymic atrophy. However, we found no significant correlation between sjTREC levels and age, probably owing to the process of thymic involution that occurs at an early stage of life in dogs.…”

mentioning

confidence: 99%

Analysis of DNA methylation of potential age-related methylation sites in canine peripheral blood leukocytes

Ito

Yoshimura

Momoi

2017

The Journal of Veterinary Medical Science

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Reliable methodology for predicting the age of mature dogs is currently unavailable. In this study, amplicon sequencing of 50 blood samples obtained from diseased dogs was used to measure methylation in seven DNA regions. Significant correlations between methylation level and age were identified in four of the seven regions. These four regions were then tested in samples from 31 healthy toy poodles, and correlations were detected in two regions. The age of another 11 dogs was predicted using data from the diseased dogs and the healthy poodles. The mean difference between the actual and calculated ages was 34.3 and 23.1 months, respectively. Further research is needed to identify additional sites of age-related methylation and allow accurate age prediction in dogs.

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“…sjTREC in humans performed slightly better than DNA methylation, with a median

(||, β false/ σ)

of 0.47 (Table ). However, the single application of sjTREC in mammals showed no correlation with age (Ito, Yoshimura, & Momoi, ).…”

Section: Resultsmentioning

confidence: 99%

The adequacy of aging techniques in vertebrates for rapid estimation of population mortality rates from age distributions

Zhao

Klaassen

Lisovski

et al. 2018

Ecology and Evolution

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As a key parameter in population dynamics, mortality rates are frequently estimated using mark–recapture data, which requires extensive, long‐term data sets. As a potential rapid alternative, we can measure variables correlated to age, allowing the compilation of population age distributions, from which mortality rates can be derived. However, most studies employing such techniques have ignored their inherent inaccuracy and have thereby failed to provide reliable mortality estimates. In this study, we present a general statistical model linking birth rate, mortality rate, and population age distributions. We next assessed the reliability and data needs (i.e., sample size) for estimating mortality rate of eight different aging techniques. The results revealed that for half of the aging techniques, correlations with age varied considerably, translating into highly variable accuracies when used to estimate mortality rate from age distributions. Telomere length is generally not sufficiently correlated to age to provide reliable mortality rate estimates. DNA methylation, signal‐joint T‐cell recombination excision circle (sjTREC), and racemization are generally more promising techniques to ultimately estimate mortality rate, if a sufficiently high sample size is available. Otolith ring counts, otolithometry, and age‐length keys in fish, and skeletochronology in reptiles, mammals, and amphibians, outperformed all other aging techniques and generated relatively accurate mortality rate estimation with a sample size that can be feasibly obtained. Provided the method chosen is minimizing and estimating the error in age estimation, it is possible to accurately estimate mortality rates from age distributions. The method therewith has the potential to estimate a critical, population dynamic parameter to inform conservation efforts within a limited time frame as opposed to mark–recapture analyses.

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Gene analysis of signal-joint T cell receptor excision circles and their relationship to age in dogs

Cited by 4 publications

References 26 publications

An Age-Associated Decline in Thymic Output Differs in Dog Breeds According to Their Longevity

An Age-Associated Decline in Thymic Output Differs in Dog Breeds According to Their Longevity

Analysis of DNA methylation of potential age-related methylation sites in canine peripheral blood leukocytes

The adequacy of aging techniques in vertebrates for rapid estimation of population mortality rates from age distributions

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