Aerobe and anaerobe facultative Gram-negative bacteria rod-shaped in the ruminal fluid of dairy cattle fed with different diets containing tropical forages

Freitas, C.E.S; Almeida, Pnm; Duarte, E. R.; Abrão, FO; Careli, Roberta Torres; Geraseev, L.C

doi:10.4067/s0301-732x2014000300017

Cited by 5 publications

(8 citation statements)

References 21 publications

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“…Bacteria belonging to the genera Proteus spp., Raoultella spp., Citrobacter spp., and Klebsiella spp. were recovered in the ruminal fluid of dairy cattle, rumen samples, and bovine fecal material [30][31][32]; their occurrence in ruminants could be expected because some of these genera, such as Pantoea, Raoultella, and Citrobacter, are reported to be cellulase-producing bacteria [10,33]. Moreover, according to Mao et al [34], in addition to Proteobacteria, Firmicutes is a predominant phylum found in rumen samples of dairy cattle.…”

Section: Resultsmentioning

confidence: 99%

Bio-Based Succinate Production from Arundo donax Hydrolysate with the New Natural Succinic Acid-Producing Strain Basfia succiniciproducens BPP7

et al. 2017

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Bio-based succinic acid production from lignocellulosic biomass is one of the attractive and prominent alternative technologies to overcome issues associated with the utilization of fossil sources. In this context, it is necessary to find new microorganisms that are able to efficiently ferment this recalcitrant feedstock. The ecological approach developed in this study enabled the isolation of Basfia succiniciproducens BPP7 from a complex rumen ecosystem. This new wild-type strain was able to synthesize up to 6.06 ± 0.05 g/L of succinate (corresponding to 0.84 ± 0.017 g of succinate per gram of consumed glucose + xylose and to 0.14 ± 0.001 g of succinate per gram of glucans + xylans present in the biomass before hydrolysis) from Arundo donax hydrolysate in separate hydrolysis and fermentation (SHF) experiments. Higher titers of succinic acid were obtained through the optimization of growth conditions. The optimal medium composition identified on the smaller scale was then used for 2.5-L batch experiments, which used A. donax hydrolysate and yeast extract as the main C and N sources, respectively. A maximal titer of 9.4 ± 0.4 g/L of succinic acid was obtained after 24 h. The overall results clearly demonstrate the potential of B. succiniciproducens BPP7 for succinate production.

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Section: Resultsmentioning

confidence: 99%

Bio-Based Succinate Production from Arundo donax Hydrolysate with the New Natural Succinic Acid-Producing Strain Basfia succiniciproducens BPP7

et al. 2017

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“…Para os animais alimentados com volumoso, a média de unidade formadora de colônia (UFC/ml) e as taxas de positividades não diferiram estatisticamente entre os bovinos de diferentes idades (p>0,05). Diferentemente, em nosso estudo conduzido com bovinos leiteiros da raça Holandesa (Freitas et al 2014), verificou-se que as bezerras apresentaram maior população dessas bactérias no ambiente ruminal, comparando-se com vacas leiteiras adultas que recebiam a mesma fonte de volumoso (silagem de sorgo).…”

Section: Cultivo E Quantificaçãounclassified

“…Essa maior população de bactérias em novilhos alimentados com grãos poderia ser justificada uma vez que esses microrganismos promovem a degradação de carboidratos mais simples (Ostling et al 2006). Bezerras leiterias alimentadas com cana-de-açúcar e ureia apresentaram elevado número dessas bactérias no fluido ruminal, o que foi relacionado com a maior concentração de carboidrato disponível no volumoso, uma vez que nessa gramínea são detectados altos níveis de sacarose, que é facilmente degradada no rúmen (Freitas et al 2014). Krause et al (2003) avaliaram o efeito da dieta sobre a população Enterobacteriaceae e verificaram grandes concentrações ao longo do trato digestório de bovinos.…”

Section: Cultivo E Quantificaçãounclassified

“…Entretanto, em nosso estudo anterior, verificou-se que vacas leiteiras adultas, alimentadas com silagem de sorgo possuíam maior ocorrência Klebsiella spp. do que bezerras alimentadas com a mesma dieta (Freitas et al 2014).…”

Section: Distribuição Dos Gêneros De Bactériasunclassified

“…são agentes relevantes na saúde pública, ocasionam diarreia e mastite aos bovinos, promovendo perdas econômicas na pecuária (Anderson 2005, Duse et al 2015. Espécies de Enterobacteriaceae podem frequentemente serem encontradas no ecossistema ruminal (Scot et al 2008, Freitas et. al, 2014.…”

Section: Introductionunclassified

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Bastonetes Gram-negativos aeróbios e anaeróbios facultativos no fluido ruminal de bovinos de corte alimentados em pastagem lignificada e em novilhos com acidose ruminal

et al. 2015

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Resumo: Objetivou-se analisar a população de bastonetes Gram negativos aeróbios e anaeróbios facultativas no suco ruminal bovinos zebuínos de diferentes categorias, alimentados em pastagem tropical, e de novilhos alimentados com alto teor de grão e sem volumosos. Foram coletados fluido ruminal de 32 vacas, 50 novilhos e 50 bezerros alimentadas em pastagem de Brachiaria spp. e de 20 novilhos com acidose ruminal. Após diluições decimais, amostras foram inoculadas em placas contendo ágar MacConkey a 39°C. Para a identificação dos gêneros mais frequentes foram utilizadas provas bioquímicas. A concentração dessas bactérias não diferiu no ambiente ruminal de vacas, novilhos e bezerros de corte alimentados com pastagem tropical lignificada. Os gêneros mais frequentemente identificados para esses animais foram Escherichia, Enterobacter e Klebsiella. Novilhos alimentados sem volumoso e com acidose apresentaram maior taxa de detecção e maior população dessas bactérias no ambiente ruminal (>6 log/ml) quando comparados aos novilhos alimentados somente em pastagem. A espécie Escherichia coli foi predominante entre as bactérias isoladas do fluido ruminal de novilhos alimentados com dieta com alta concentração de grãos e com acidose (p<0,01). Constatou-se que em bovinos de corte, criados em pastagem tropical lignificada, a população desses microrganismos é baixa no ambiente ruminal e com maior diversidade de gêneros bacterianos. Entretanto em novilhos confinados e alimentos sem volumoso, apresentando acidose ruminal subaguda, ocorre desequilíbrio populacional com aumento da população de E. coli.

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Systematic Analysis of Escherichia coli Isolates from Sheep and Cattle Suggests Adaption to the Rumen Niche

Pang

Liu

Zhang

et al. 2020

Appl Environ Microbiol

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The commonly used laboratory bacterium Escherichia coli normally does not produce and secrete cellulases due to its complex bilayer membrane structure and poor secretory apparatus. In our previous study, the cellulolytic E. coli strain ZH-4 with extracellular cellulase activity was found in the bovine rumen. In this study, we demonstrate that the secretion of cellulase is a common feature of E. coli isolated from the rumen, such as sheep and cattle. Physiological phenotype characterization of these E. coli isolates together with genome, transcriptome and comparative genomics analysis suggested their adaption to the rumen niche. The faster growth rate of the isolated strains under aerobic conditions meets the competitive requirements of the strains in rumen microecosystem, while anaerobic accumulation of reduced H2 and succinate was hypothesized to be the results of adaptation to the rumen environment. The cellulase secretion increased significantly when the molecular chaperone genes ibpA and ibpB was overexpressed. These were also revealed by the transcriptomic data. A possible reason of cellulase secretion by E. coli isolates was proposed based on the transcriptomic data and molecular experiments. Importance As an important intestinal microorganism, E. coli is present in the intestinal tract of animals and many other environments. However, it normally does not produce and secret cellulases due to its complex bilayer membrane structure and poor secretory apparatus. Here, we proved that E. coli is widely present in the rumen of sheep and cattle. Systematic analysis of the isolates indicated that they have adapted to the rumen niche, with phenotypes including secretion of cellulase and fermentative accumulation of succinate and H2. The finding that overexpression small heat shock protein genes ibpA and ibpB could facilitate cellulase BcsZ secretion, which provides a possible insight into the protein secretion mechanism of rumen colonizing E. coli.

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Aerobe and anaerobe facultative Gram-negative bacteria rod-shaped in the ruminal fluid of dairy cattle fed with different diets containing tropical forages

Cited by 5 publications

References 21 publications

Bio-Based Succinate Production from Arundo donax Hydrolysate with the New Natural Succinic Acid-Producing Strain Basfia succiniciproducens BPP7

Bio-Based Succinate Production from Arundo donax Hydrolysate with the New Natural Succinic Acid-Producing Strain Basfia succiniciproducens BPP7

Bastonetes Gram-negativos aeróbios e anaeróbios facultativos no fluido ruminal de bovinos de corte alimentados em pastagem lignificada e em novilhos com acidose ruminal

Systematic Analysis of Escherichia coli Isolates from Sheep and Cattle Suggests Adaption to the Rumen Niche

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