Bio-Based Succinate Production from Arundo donax Hydrolysate with the New Natural Succinic Acid-Producing Strain Basfia succiniciproducens BPP7

Abstract

Bio-based succinic acid production from lignocellulosic biomass is one of the attractive and prominent alternative technologies to overcome issues associated with the utilization of fossil sources. In this context, it is necessary to find new microorganisms that are able to efficiently ferment this recalcitrant feedstock. The ecological approach developed in this study enabled the isolation of Basfia succiniciproducens BPP7 from a complex rumen ecosystem. This new wild-type strain was able to synthesize up to 6.06 ± 0.05 g/L of succinate (corresponding to 0.84 ± 0.017 g of succinate per gram of consumed glucose + xylose and to 0.14 ± 0.001 g of succinate per gram of glucans + xylans present in the biomass before hydrolysis) from Arundo donax hydrolysate in separate hydrolysis and fermentation (SHF) experiments. Higher titers of succinic acid were obtained through the optimization of growth conditions. The optimal medium composition identified on the smaller scale was then used for 2.5-L batch experiments, which used A. donax hydrolysate and yeast extract as the main C and N sources, respectively. A maximal titer of 9.4 ± 0.4 g/L of succinic acid was obtained after 24 h. The overall results clearly demonstrate the potential of B. succiniciproducens BPP7 for succinate production.

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

“…Environmentally friendly energy resources for alternative fuels have increased in the last decade, with the aim of progressively replacing fossil fuels and reducing the amount of greenhouse gases released into the atmosphere (Hoyer et al 2009;Menegol et al 2016;Ventorino et al 2017). Bioethanol, produced from lignocellulosic biomass, is a possible alternative to fossil fuels used in transportation (Saini et al 2015).…”

Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse

“…Agri-food wastes are additional cheap, sustainable and attractive substrate for the production of biopolymers or other high value-added products [21]. Many studies have assessed the recycling and the potential use of agri-food wastes and byproducts or dedicated energy crops for producing polyhydroxyalkanoate (PHA) [22,23], succinic acid [24][25][26][27], biofuels and biogas [19,[28][29][30][31][32] as well as biological hydrogen and volatile fatty acids [33,34]. Natural environments represent important sources of microbial strains that exert interesting enzymatic activities for biotechnological applications [35].…”

Methyl t-butyl ether-degrading bacteria for bioremediation and biocontrol purposes

“…Bacterial isolates were identified using a polyphasic approach on the basis of their phenotypic, biochemical, and molecular characterization. Isolates were preliminarily characterized on the basis of their microscopic morphology (phasecontrast microscopy, shape, dimension, and presence of spores) and biochemical features (Gram stains and catalase activity) as previously reported [18].…”