Methyl t-butyl ether-degrading bacteria for bioremediation and biocontrol purposes

Abstract

A total of fifteen potential methyl t-butyl ether (MtBE)-degrading bacterial strains were isolated from contaminated soil. They have been identified as belonging to the genera Bacillus, Pseudomonas, Kocuria, Janibacter, Starkeya, Bosea, Mycolicibacterium, and Rhodovarius. Bacillus aryabhattai R1B, S. novella R8b, and M. mucogenicum R8i were able to grow using MtBE as carbon source, exhibiting different growth behavior and contaminant degradation ability. Their biocontrol ability was tested against various fungal pathogens. Both S. novella R8b and B. aryabhattai were effective in reducing the development of necrotic areas on leaves within 48 hours from Botritys cinerea and Alternaria alternata inoculation. Whereas, M. mucogenicum effectively controlled B. cinerea after 72 hours. Similar results were achieved using Pythium ultimum, in which the application of isolated bacteria increased seed germination. Only M. mucogenicum elicited tomato plants resistance against B. cinerea. This is the first report describing the occurrence of bioremediation and biocontrol activities in M. mucogenicum, B. aryabhattai and S. novella species. The production of maculosin and its antibiotic activity against Rhizoctonia solani has been reported for first time from S. novella. Our results highlight the importance of multidisciplinary approaches to achieve a consistent selection of bacterial strains useful for plant protection and bioremediation purposes.

Biochemical and structural insight into the chemical resistance and cofactor specificity of the formate dehydrogenase from Starkeya novella

“…Starkeya novella is known for its extensive metabolic flexibility, since it is a mesophilic organism able to grow not only on single carbon compounds (such as methanol and formate) and sulfur derivatives (e.g. thiosulfate) [49], but also in soils contaminated with toxic organophosphate insecticides [50] and organic fuel additives [51]. Bacteria that survive a variety of environmental stresses from extreme natural conditions to artificial pollutants, could harbour valuable biocatalysts for the manufacture of platform chemicals [52,53].…”

Caracterización transcriptómica de bacterias relacionadas con eubiosis y disbiosis en pacientes con y sin carcinoma escamocelular oral

“…En el presente estudio S. novella expreso altos niveles de transcritos de genes como Factor de elongación de traslación Tu, Factor de elongación de traslación G, Subunidad beta de la ARN polimerasa dirigida por ADN relacionados con metabolismo de carbohidratos, nucleótidos, aminoácidos, y energía que podrían estar asociados a la regulación de los estados saludables. En el momento actual S. novella está pobremente caracterizada, sin embargo, algunos análisis sugieren su importancia en biopelículas en humanos 35 . En relación con E. asburiae se encontraron expresiones bajas de genes como el Factor de elongación de traslación Tu, Subunidad B de ADN girasa, asociados con procesos de metabolismo de carbohidratos, aminoácidos, nucleótidos y energía.…”

Effects of Lead on Petrol Degradation Efficiency of Bacteria Isolated from Soils in Zhuhai, Guangdong, China

“…Similar strains of A. denitrificans, such as A. denitrificans strain SP1, A. denitrificans ASU-035, A. xylosoxidans subspecies denitrificans strain EST4002 and A. denitrificans strain TB, have recently been identified to be able to degrade di(2-ethylhexyl)phthalate, pyrene, 2, 4-dichlorophenoxyacetic acid and reduce nitric oxide, respectively [29][30][31][32]. Although the genera Mycolicibacterium, such as M. mucogenicum, M. frederiksbergense IN53, M. vanbaalenii, have been reported to be able to degrade methy t-butyl ether, polychlorinated biphenyls and petroleum hydrocarbons [33][34][35], the strain M. phocaicum N4, which shows 99.71% sequence homology to the isolated B-10 strain in this study via 16S rRNA sequencing technique, has recently been isolated from human bronchial aspirate from patients showing tuberculosis symptoms [36].…”