Adaptability of tissue-cultured Dendrobium orchid planlets on planting media and its position during acclimatization process

Abstract

The acclimatization process of cultured plants needs special attention because it is a critical period for adaptation to In-Vivo conditions. The environmental changes and the imperfection of planlet anatomy are important things that need to be considered to support the growth of Dendrobium orchid planlet. The aim of this study was to determine the effect of planting media type and its positioning on the Dendrobium orchid adaptability during acclimatization process. This study was conducted from March to Mei 2017 in AIAT North Sumatra tissue culture laboratory and screen house. The experimental design used in this study was a randomized group design with five replications. The treatment consisted of planting media and pot position model. The planting media combination were fern+wood charcoal (M0); fern+coconut fibre (M1); ferns+broken bricks (M2); fern+wood charcoal+coconut fibre (M3); and ferns+wood charcoal+coconut fibre+broken bricks (M4) while the pot position model were laying (P0) and hanging (P1) model. Morphological parameters observed were growing plant percentage, plant height, leaves number, roots number, and roots length. The results showed that the best planting media and pot position combination supported the Dendrobium orchid growth were found in ferns and wood charcoal (M0) and ferns and bricks (M2) with lying model (P0).

Effect of organic additives on regeneration of orchid hybrid (Dendrobium ‘Bertachong’ X Dendrobium ‘Blackspider X Sutiknoi’)

“…3, plantlets which were cultured on MS medium with 15% mungbean sprout extract, 1 mg.L -1 NAA, and 2% sucrose had the most number and the longest roots (Table 3), followed by plantlets which were cultured at VW medium with 15% mungbean sprout extract, 1 mg.L -1 NAA, and 2% sucrose, and then plantlets which cultured at MS medium with 15% coconut water, 1 mg.L -1 NAA, and 2% sucrose (Table 3). Acclimatization stage of cultured plants is a critical period for adaptation to ex vitro condition, so acclimatization stage needs special attention [26]. For adaptation to ex vitro conditions, selected plantlets must have sufficient number and length roots, so they can survive well at ex vitro condition.…”

Sterilization method of contaminated oil palm plantlets affects the survival rate during acclimatization

“…P3 treatment resulted in low fungal recontamination, good plantlet vigor, and a high survival rate, as the plantlets managed to withstand the challenges during acclimatization. Dipping plantlets on sterilant before acclimatization is a standard method (Nasution et al, 2020;. This step is essential to minimizing microbial attack during acclimatization.…”

​The Growth Response of Banana cv. Barangan (Musa acuminata L.) from Tissue Culture with Organic Manure and Arbuscular Mycorrhizal Fungi

“…Tissue culture cultivation is a way to produce uniform plant seedlings in large quantities and free from disease. Acclimatization is the last and most crucial tissue culture stage that aims to get used to plantlet living in natural environments such as greenhouses and planting grounds than previously lived in con trolled environmen tal circumstances (Duan et al, 2020;Nasution et al, 2020). The availability of nutrients needs to be considered because acclimatization is a critical and restrictive stage in plant micropropagation (Ferreira et al 2017).…”

“…The availability of nutrients needs to be considered because acclimatization is a critical and restrictive stage in plant micropropagation (Ferreira et al 2017). Organic manures including compost as providers of macro and micronutrients can increase the pH and soil structure by improving organic matter and soil ability in maintaining groundwater (Nugroho et al 2017;Samanhudi et al 2018a;Samanhudi et al, 2017a). The addition of organic manure and compost will add plant nutrients to support growth and determine banana plantlet acclimatization success.…”