Porang (Amorphophallus muelleri Blume.) usually used to functional food raw materials, caused its high glucomant content. Limitation of conventional propagation both vegetative and generative affected to decreasing of porang production to fulfil market demand. To solve the problem, plant tissue culture technique was chosen to rapid propagate bulbils of porang. So, the aim of this research to determine the combination and concentration of Plant growth Regulators (PGRs), such as 6-Benzylaminopurine (BAP), Naphthaleneacetic Acid (NAA), and Thidiazuron (TDZ) for quality of callus, also growth of callus and shoots for rapid propagation of porang, cause PGRs are one of factors that affect the success of in vitro propagation. Experimental design of this research was completely randomized designed with statistical analysis using analysis of variance (Anova) one way. Difference combination and concentration of cytokinin and auxin as manipulation variables, which several parameters growth of callus, shoots, and roots was observed to indicate the rapid organogenic of porang. The optimal shoot induction medium was Murashige and Skoog (MS) media with 5.0 mg.L-1 BAP and 0.2 mg.L-1 NAA on which number of shoots and shoot height reached 15±1.8 and (7.2±1.86) cm. After shoot formation stage, the proper media for rooting stage was determined, which was Murashige and Skoog (MS) media with 1.0 mg.L-1 NAA resulted the rooting rate reached 100% and roots appear earlier. Optimal combination and concentration of PGRs on micropropagation of Amorphophallus muelleri Blume. from bulbils explant should be known to increase the production of porang, which can fulfil the market demand.
Dendrobium is a genus of orchids that has been widely planted, both domestically and internationally. Orchid conventional breeding is generally done to produce new variants, but seeds from crosses are generally difficult to germinate. To solve this problem, tissue culture has long been used to germinate orchid seeds. This study aims to obtain a suitable medium composition for growing seeds from crosses of Dendrobium 'Bertachong' X Dendrobium 'Blackspider x Sutiknoi'. The seeds were grown on two different media types, MS (Murashige-Skoog) and VW (Vacin-Went), with the addition of 2% sucrose and with or without the addition of organic extracts (either mungbean sprouts extract or coconut water). Plantlets were sub-cultured every 4 weeks for 2 times on the same medium but with the addition of activated charcoal, and followed by a third subculture with the addition of 1 mg.L -1 NAA (Naphthalene Acetic Acid) in each medium. The best growth shown by fast regeneration and good seedling quality (multiple buds and roots formed) was obtained on MS medium with the addition of 2% sucrose and 15% mungbean sprouts extract.
Objectives: This study aimed to examine the effect of a laparoscopic cystectomy on ovarian reserve assessed by measuring the levels of serum follicle stimulating hormone (FSH) and anti-Müllerian hormone (AMH). Materials and Methods: This prospective, analytical study measured the changes of basal FSH and AMH in endometrioma patients undergoing laparoscopic cystectomy in Dr. Hasan Sadikin Hospital from June to August 2016. An informed written consent was obtained from all patients prior to the study. Their FSH and AMH serum levels were examined one month before and after the surgery. Results: A total of 31 patients were included in the study and divided into two groups based on two age ranges of 20-35 years (n=16) and 36-42 years (n=15). It was found that the FSH serum levels increased whereas the AMH serum levels decreased in both groups after laparoscopic cystectomy (P<0.001). Conclusions: Laparoscopic cystectomy of patients with reduced endometrioma ovarian reserve was characterized by elevation of FSH serum levels and reduction of AMH serum levels.
Nilam Aceh ‘Sidikalang’ (Acehnese Patchouli) merupakan salah satu anggota kelompok dari Lamiaceae, famili mint. Minyak aromatik yang biasa disebut juga dengan minyak patchouli dapat diproduksi dari daunnya, yang mana bernilai tinggi dalam industri parfum dan aromaterapi, karena wangi aromatik yang segar. Minyak patchouli juga memiliki variasi senyawa fitokimia yang memiliki efek terapeutik, seperti antimikroba, antidepresan, anti-inflamasi, dan antioksidan. Penelitian ini bertujuan untuk menentukan protocol yang efisien dan reprodusibel untuk refenerasi tak langsung dari eksplan daun dan banyak tunas dari eksplan nodus. Organogenesis tak langsung telah dilakukan menggunakan medium MS (Murashige-Skoog) dengan beberapa perlakuan, seperti NAA 0,5 mg/L + BAP 0,1 mg/L, NAA 1 mg/L + BAP 0,1 mg/L, dan NAA 1,5 mg/L + BAP 0,1 mg/L, sedangkan tunas majemuk yang dihasilkan dari eksplan nodus dikulturkan pada media MS dengan berbagai variasi konsentrasi BAP, yakni 0,5 mg/L, 1,0 mg/L, dan 1,5 mg/L kemudian, untuk tahap perakaran dari tunas, tunas akan dikulturkan pada media ½ MS tanpa Zat Pengatur Tumbuh (ZPT) dan media MS dengan beberapa perlakuan, yakni tanpa Zat Pengatur Tumbuh (ZPT), IBA (Indole-3-Butyric Acid) 0,5 mg/L, dan NAA (Naphthalene Acetic Acid) 0,5 mg/L. Selanjutnya, planlet yang dihasilkan dari tahap perakaran secara in vitro akan diaklimatisasi pada kombinasi tanah : kompos (1:1). Sebagai tambahan, tunas in vitro juga akan ditanam secara langsung sebagai stek tunas mikro pada kombinasi tanah : kompos (1:1). Perlakuan optimal untuk organogenesis tak langsung menggunakan media MS dengan NAA 1 mg/L + BAP 0,1 mg/L. Dalam perbanyakan tunas majemuk dari eksplan nodus, media MS dengan BAP 0,5 mg/L merupakan media optimum. Tunas yang dikulturkan pada media ½ MS untuk tahap perakaran, tumbuh membentuk planlet dengan morfologi akar normal. Secara keseluruhan, planlet nilam lebih cepat terbentuk melalui penanaman langsung stek tunas mikro pada kondisi ex vitro dibandingkan melalui tahap perakaran in vitro.
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