ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer

Abstract

SUMMARY OBJECTIVE ADAMTS4 is a member of the ADAMTS4 family, which secretes proteinases. The mechanism of tumor metastasis may be correlated to its promotion of angiogenesis. It was determined whether ADAMTS4 participates in colorectal cancer progression. Methods The expression in clinical samples and CRC cell lines was investigated. Using immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and RT-PCR, the expression of ADAMTS4 was determined in colorectal tumors of different cancer stages and anatomic sites, and in three cell lines of different aggressiveness. Results The overexpression of ADAMTS4 was observed in tissue samples by IHC, and this was mainly located in the cytoplasm, as detected by FISH. The qRT-PCR and western blot analyses further supported the clinical sample findings. Conclusion The present data support the notion that the overexpression of ADAMTS4 in CRC might be useful as a non-invasive biomarker for detecting CRC in patients.

Dextran-Sulfate-Sodium-Induced Colitis-Ameliorating Effect of Aqueous Phyllanthus emblica L. Extract through Regulating Colonic Cell Gene Expression and Gut Microbiomes

“…The expressions of chemokine and inflammation cytokine MCP-1 (CCL2) and IL11, which belong to the pathway of the cytokine–cytokine receptor interaction and TNF signaling pathway, increased in the DSS-induced model group and depressed in the APE treatment group ( p < 0.01). Cytokine antagonists and blockade of chemokines were adopted in early cancer therapy, such as blockade of the CCL2–CCR2 axis suppressing tumor-associated macrophage (TAM) recruitment proven in a preclinical model. , Researchers have found that the upregulated expressions of genes Adam12, Adamts1, and Adamts4 were related with immune cell infiltration as well as colorectal cancer, and Gfpt2 promoted the magnification, metastasis, and invasion of colorectal cancer cells. − Nr4a3 as a member of the nuclear receptor subfamily 4 plays a significant part in cellular inflammation and function. Previous studies indicated that Nr4a3 plays a pro-inflammatory role in the development of human osteoarthritis as well as has been detected to cause endothelial disorder in adipose-tissue-derived stromal cells. , In this study, the above-mentioned gene expressions were upregulated in DSS-treated mice and adjusted to the low level, like the normal group, in the APE consumption group ( p < 0.05).…”

The expression of SERPINE1 in colon cancer and its regulatory network and prognostic value

“…MMP19 (matrix metallopeptidase 19) is related to epithelial cell proliferation, enterocyte migration and poor prognosis [ 26 , 27 ]. ADAMTS4 is highly expressed in colon cancer cells, and research suggested that ADAMTS4 can promote cancer progression through macrophages [ 28 , 29 ]. SERPINE1 is notably positively correlated with these three genes, that is, SERPINE1 and these three genes may jointly played important roles in the progression of colon cancer.…”

Tratamento videolaparoscópico para neoplasia de cólon direito: relato de caso

“…No Brasil, aparece entre os cinco primeiros tipos de câncer mais frequentes (INCA, 2018). O risco deste tipo de neoplasia aumenta com a faixa etária, manifestando-se em mais de 50% dos casos após 60 anos (Balmagambetova et al, 2020;Shang et al, 2020). No presente relato trata-se de um paciente do sexo masculino com 54 anos de idade, discordando um pouco da literatura.…”