Activity of leaf extracts of Coix lachryma Linn. and Asparagus cochinchinenesis Linn. as breast anticancer drugs

Abstract

In the current economic crisis, the use of plant medicine for cancer prevention should be investigated. Coix lachryma Linn and Asparagus cochinchinensis Linn are among eleven of species of medicinal plants that are noted as plant medicine for cancer in Indonesia, although their mechanism of action are still unknown. The eleven plants were screened using in vitro methods, Sulforhodamin B against breast cancer cells (MCF-7) and skin (KB). The research included a maceration process using ethanol as solvent and an anti-cancer testing process in vitro using Sulforhodamin B indicated by the value of percentage viability. Extracts were classed as being 'active anticancer' if they showed IC50 values below 100 ppm.. Coix lachryma Linn. and Asparagus cochinchinensis Linn. show breast and skin anticancer activity with IC50 values 6.51 ppm and 11.3 ppm of MCF-7 cells. The ethanol plant extracts were further extracted using various solvents with increasing polarity: n-hexane, methylene chloride, and ethyl acetate. The methylene chloride extract of Coix lachryma Linn. had IC50 = 2.75 ppm against MCF-7 cells. Against KB cells, methylene chloride extracts of Coix lachryma Linn. gave IC50 = 5.16 ppm. For Asparagus cochinchinensis Linn., an ethyl acetate extract had IC50 = 3.70 ppm against KB cancer cells and IC50 = 9.80 ppm against MCF-7 cancer cells. These data indicated that both plants can be used as anticancer drugs on breast and skin cancers.

Standardization in ethanolic extract of Paronema canescens leaves

“…The bacterial contamination test was applied to determine the number of microorganisms and the presence or absence of certain bacteria in the extract [23]. The maximum limit for the number of bacterial colonies is 10 4 cfu/mL.…”

Efek Anti Bakteri Ekstrak Kulit Jeruk Nipis (Citrus Aurantifolia) Terhadap Salmonella Typhi Secara in Vitro

“…Alkaloid dari tanaman kebanyakan merupakan senyawa amina tersier dan yang lainnya terdiri dari nitrogen primer, sekunder, dan quartener (Poither,2000). Semua alkaloid mengandung paling sedikit satu atom nitrogen yang biasanya bersifat basa dan sebagian besar atom nitrogen ini merupakan cincin aromatis (Mustarichie, 2011). Mekanisme kerja alkaloid sebagai antibakteri adalah dengan cara mengganggu komponen penyusun peptidoglikan pada sel bakteri, sehingga lapisan dinding sel tidak terbentuk secara utuh dan menyebabkan kematian sel tersebut (Robinson,1991).…”

In silico analysis of trisindoline 1 as tyrosinase inhibitors

“…Molecular docking is used to study the interactions that occur between inhibitors and the active site of the enzyme [17]. This method can analyse the position of a compound to the enzyme and the chemical bonds involved so that the activity of the compound to an enzyme can be predicted [18]. This study aims to analyse the potential of trisindoline 1 as a candidate for a new tyrosinase enzyme inhibitor.…”