Activity of antioxidant enzymes in the skin during surgical wounds

Abstract

Full-thickness skin wounds (460 mm(2)) in rats were associated with increased blood chemiluminescence and neutrophil infiltration of the wound tissue and surrounding skin (recorded by myeloperoxidase activity). Activities of glutathione peroxidase and glutathione S-transferase in the skin and wound tissue increased on days 4 and 8. A correlation was revealed between activities of these enzymes and myeloperoxidase activity. Activities of myeloperoxidase and catalase increased in patient's skin excised during plastic surgeries of more than 2.5 h duration.

Systematic investigation of ethanolic extract from Leea macrophylla : Implications in wound healing

“…Such is the dilemma with the ubiquitousness of inflammatory responses that both their over expression and under expression lead to deleterious consequences like susceptibility to innocuous environmental factors, severe damage and even tumorogenesis. Therefore, curtailing the influence of ROS on wound openings could be an important alternative strategy (White and Heckler, 1990;Mikhal'chik et al, 2006). ROS can be eliminated by enhancing expression of antioxidant enzymes like SOD, GSH and CAT or by scavenging preexisting free radicals such as LPO products and NO.…”

Evaluation of Antioxidant, Toxicological and wound healing Properties of Hibiscus rosa-sinensis L. (Malvaceae) ethanolic leaves extract on different Experimental animal models

“…The increased hydroxyproline content in dead space wounds indicates faster collagen turnover which leads to rapid healing with concurrent increase in the breaking strength of the treated wounds and thus also indicating stabilization of collagen fibres. 64 Oxidative stress is caused by overproduction of reactive oxygen species (ROS), thereby causing delay in the wound healing process. Therefore, in healing of chronic wounds elimination of ROS could be an important strategy and estimation of antioxidants like superoxide dismutase (SOD), catalase (CAT) etc., in granulation tissue becomes relevant as they help destroy free radicals and hasten the process of wound healing.…”

Activity of Antioxidant Enzymes in the Wound in Patients with Deep Burns

“…In the previous study on a model of excision wound and burn injury in rats we found that CAT activity in the wound tissue and in the adjacent skin virtually did not increase in response to trauma [2], which was in good agreement with the data on negative effect of exogenous CAT on the rate of surgical wound healing in mice [9]. It seems, that the complex of antioxidant enzymes in the skin and wound tissue of mice and rats supports optimal concentration of H 2 O 2 needed for redox stimulation of wound healing without induction of CAT (for example, at the expense of glutathione peroxidase activation [2,13]).…”

“…It seems, that the complex of antioxidant enzymes in the skin and wound tissue of mice and rats supports optimal concentration of H 2 O 2 needed for redox stimulation of wound healing without induction of CAT (for example, at the expense of glutathione peroxidase activation [2,13]). By contrast, analysis of antioxidant enzymes in human skin specimens resected during plastic surgery showed a signifi cant increase of CAT activity by the end of the intervention [2]. According to our data, activity of glutathione peroxidase in human skin is at least 5-fold lower than in the rat skin.…”