Activity of Antioxidant Enzymes in the Wound in Patients with Deep Burns

Mikhalchik, Elena V.; Piterskaya, J. A.; Lipatova, V. A.; Penkov, L. Yu.; Ibragimova, G. A.; Korkina, Liudmila

doi:10.1007/s10517-009-0614-z

Cited by 5 publications

(3 citation statements)

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“…Vacuum, drainage, or other external devices were used in 124 of the 280 (44%) studies and thus were the most frequently used methods of sample collection 22–27,29–146 ; the vast majority of sample collections using this method (75%, 103/138), were from surgical wounds (Figure 2B). Three other frequently used methods were extraction from absorbent material (31%, 86/280),…”

Section: Resultsmentioning

confidence: 99%

Wound fluid sampling methods for proteomic studies: A scoping review

Harvey

Mellody

Cullum

et al. 2022

Wound Repair Regeneration

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Understanding why some wounds are hard to heal is important for improving care and developing more effective treatments. The method of sample collection used is an integral step in the research process and thus may affect the results obtained. The primary objective of this study was to summarise and map the methods currently used to sample wound fluid for protein profiling and analysis. Eligible studies were those that used a sampling method to collect wound fluid from any human wound for analysis of proteins. A search for eligible studies was performed using MEDLINE, Embase and CINAHL Plus in May 2020. All references were screened for eligibility by one reviewer, followed by discussion and consensus with a second reviewer.Quantitative data were mapped and visualised using appropriate software and summarised via a narrative summary. After screening, 280 studies were included in this review. The most commonly used group of wound fluid collection methods were vacuum, drainage or use of other external devices, with surgical wounds being the most common sample source. Other frequently used collection methods were extraction from absorbent materials, collection beneath an occlusive dressing and direct collection of wound fluid. This scoping review highlights the variety of methods used for wound fluid collection. Many studies had small sample sizes and short sample collection periods; these weaknesses have hampered the discovery and validation of novel biomarkers. Future research should aim to assess the reproducibility and feasibility of sampling and analytical methods for use in larger longitudinal studies.

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Section: Resultsmentioning

confidence: 99%

Wound fluid sampling methods for proteomic studies: A scoping review

Harvey

Mellody

Cullum

et al. 2022

Wound Repair Regeneration

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“…This cytokine is a potent chemoattractant and stimulator of neutrophils [6]. Our previous studies showed that intact neutrophils are present in the wound of burn patients even 3 weeks after injury [2]. Surgical cleaning of the burn wound and skin autografting can accelerate wound healing and prevent secondary necrosis [8].…”

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confidence: 99%

Comparative Study of Cytokine Content in the Plasma and Wound Exudate from Children with Severe Burns

et al. 2009

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The content of 27 cytokines was measured in blood plasma from 19 children with severe uncomplicated burns (group 1) and complicated burns (septic toxemia, toxemia, and pneumonia; group 2). Before surgical treatment (day 4 (+/-2) after burn), significant differences were found in the concentrations of interleukin-1 receptor antagonist, interleukin-6, interleukin-8, interleukin-10, tumor necrosis factor-alpha, interferon-gamma, MCP-1, and granulocyte colony-stimulating factor. Cytokine concentration in group 2 patients was much higher than in group 1 patients and healthy children. The concentrations of interleukin-6, interleukin-8, and MCP-1 in group 1 patients significantly surpassed the normal level. Cytokine concentration in the plasma and wound exudates and myeloperoxidase activity in wound exudates from 4 patients of group 2 were measured over 18 days after burn. The inflammatory response was characterized by an increase in the content of interleukin-1beta, interleukin-8, MCP-1, tumor necrosis factor-alpha, MIP-1alpha, and granulocyte-macrophage colony-stimulating factor in the wound (as compared to that in the plasma). Activity of myeloperoxidase in all patients was shown to correlate with the amount of MIP-1alpha (r=0.47), tumor necrosis factor-alpha (r=0.47), and granulocyte-macrophage colony-stimulating factor (r=0.55, p<0.05). Interleukin-8 concentration was beyond the limits of calibration. No correlation was found between the concentration of any of 27 cytokines in blood plasma and exudate. Our results indicate that during active surgical treatment, the wound serves as the source of inflammatory cytokines. Cytokines play a role in the systemic response and increase the degree of local inflammation, which modulates the number and activity of wound neutrophils.

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“…Blood test: The pathogenesis of burn disease is strongly influenced by decreased antioxidant activity (AOA). In burns, AOA levels decrease significantly in blood serum and membranes of erythrocytes, providing indirect confirmation of a decrease in endogenous antioxidant levels [31,32]. Based on Table 5, burns (control group (II)) showed a decrease in SAA.…”

Section: The Experiments Modeled On a Thermal Burnmentioning

confidence: 99%

Wound-healing activity of polyhexamethyleneguanidine hydrochloride hydrogel and extract of Bergenia crassifolia on thermal burn simulation

Стельмах

Ochirov²,

Grigor'eva³

et al. 2022

Mong. J. Chem.

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The results of a study of the wound healing activity of a composition based on the hydrogel of polyhexamethylene guanidine hydrochloride and Bergenia crassifolia extract, under conditions of modeling thermal burns in laboratory animals (rats), are presented. It was found that the composition affects the change in the summary antioxidant and leukocyte activity towards the normalization of these indicators. Morphological analysis of the slices showed that, under the influence of a polyhexamethylene guanidine hydrogel composition and B.crassifolia extract, healing proceeds more intensively than in the control group and is manifested by a smaller thickness of the leukocyte-necrotic scab, accelerated epithelization, and complete closure of the skin defect.

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Activity of Antioxidant Enzymes in the Wound in Patients with Deep Burns

Cited by 5 publications

References 10 publications

Wound fluid sampling methods for proteomic studies: A scoping review

Wound fluid sampling methods for proteomic studies: A scoping review

Comparative Study of Cytokine Content in the Plasma and Wound Exudate from Children with Severe Burns

Wound-healing activity of polyhexamethyleneguanidine hydrochloride hydrogel and extract of Bergenia crassifolia on thermal burn simulation

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