Activité antidrépanocytaire et thermodégradation des anthocyanes extraits de Sterculia quinqueloba et Ficus capensis

Mpiana, P. T.; Balanganayi, E. K.; Kanangila, A. B.; Kalonda, E. M.; Ngbolua, Koto-te-Nyiwa; Tshibangu, Damien S. T.; Atibu, Emmanuel K.; Lumbu, J. B. S.

doi:10.4314/ijbcs.v3i3.45342

Cited by 8 publications

(8 citation statements)

References 11 publications

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“…These polyphenolic compounds are known to be responsible for the anti-sickling activity. This hypothesis has been reported in the literature by many authors (Mpiana et al, 2009b;Kambale et al, 2013;Sawadogo et al, 2017). However, other authors claim that phenylalanine, p-hydroxybenzoic acid and its derivatives as well as the maslinic, oleanolic and betulinic acids would be at the basis of the activity of anti-sickling extracts of plants (Tshibangu et al, 2011).…”

Section: Discussionsupporting

confidence: 68%

Evaluation of the anti-sickling activity of Newbouldia laevis P. Beauv extracts

Dermane¹,

Kpegba²,

Metowogo³

et al. 2019

Int. J. Bio. Chem. Sci

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Sickle cell disease is a genetic disease very expanded all over the African block. In Togo like most African countries, it's one of major causes of morbidity and mortality. The available treatments are very ornamental and are most of the time remedy than cure. However, in our areas, the sicks use medicinal plants for treating themselves. It's in this scope that this study has been conducted in sight of assessing the anti-sickling activity of Newbouldia laevis extracts (leaves, roots and stem barks) based on Emmel test using 2% sodium metabisulphite. The extracts were also studied for its in vitro antioxidant activity using 2,2-diphenyl-1picrylhydrazyl (DPPH) and 2,2'-azobis (2-amidinopropane hydrochloride) (AAPH) methods. The results obtained show that the roots and the stem barks extracts have a comparable efficiency. These extracts enabled us to reduce the sickling rate respectively at 17% and 16% against 78% for the control that was incubated in the presence of 0.9% sodium chloride. The leaves extract led to hemolysis and hemolysing concentration was 5mg/ml. Light antioxidant activity in vitro was obtained for the two extracts. Our outcomes proved that in vitro, roots and stem barks of Newbouldia laevis extracts have an anti-sickling activity contrary to the hemolysing leaves extract.

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Section: Discussionsupporting

confidence: 68%

Evaluation of the anti-sickling activity of Newbouldia laevis P. Beauv extracts

Dermane¹,

Kpegba²,

Metowogo³

et al. 2019

Int. J. Bio. Chem. Sci

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“…is indicates that the information given by traditional healers is validated by in vitro tests. e same results were found in plants used in the management of sickle disease in other Congolese cities [4][5][6]14,[23][24][25][26][27][28][29][30][31][32][33].…”

Section: In Vitrosupporting

confidence: 66%

Ethnopharmacological Survey and Antisickling Activity of Plants Used in the Management of Sickle Cell Disease in Kikwit City, DR Congo

Kitadi¹,

Mazasa

Tshibangu

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Sickle cell disease or drepanocytosis is the most known hemoglobin abnormality in the world. Recently, many medicinal plants used in the management of sickle cell disease in African traditional medicine have shown in vitro antisickling activity. Objective. This research study aims to document some Congolese plant species used in the management of sickle cell disease in Kikwit city and investigate their pharmacological property in vitro. Methods. A cross-sectional survey was done from June 2015 to March 2016 among 26 traditional healers in Kikwit city, Democratic Republic of Congo. Emmel test was used to assess in vitro antisickling activity. Habitat, morphological, biological types, phytogeographical distribution, local names, and used parts of these plant species were also determined. Results. Obtained results show that 23 plant species belonging to 16 families are used. The leaves represent the most used part (70%). Antisickling investigation showed that aqueous extracts of 18 plants (78%) exhibit a change in the shape of a sickle cell into a normal one with a normalized rate of at least 70%, confirming the in vivo effect observed by traditional healers when used as herbal medicine traditionally in the management of sickle cell disease. The evaluation of perimeter, surface, and radius of untreated and treated sickle red blood cells showed a significant difference ( p < 0.05 ) as modification indicators of the red blood cell shape. Alchornea cordifolia, Alternanthera bettzickiana, Annona senegalensis, Dissotis brazzae, Hypoxis angustifolia, and Justicia secunda presented a very high antisickling activity with normalization >70%. Otherwise, Dissotis brazzae was the most active plant with a minimal concentration of normalization (MCN) of 11 μg/mL and a maximal normalization rate (NRmax) of 86%. Conclusion. Almost 80% of the plants studied showed interesting antisickling activity and justified their use in traditional medicine. The isolation of the molecules responsible for the biological activity of the most active plants and the determination of their chemical structures is under investigation.

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“…The minimal concentration corresponding to the maximal normalization rate is called minimal concentration of normalization. A low value of the MCN is a good indication of the antisickling activity [17][18][19]21].…”

Section: Antisickling Activity Of Aqueous Extractsmentioning

confidence: 99%

“…From a diversified and large Congolese flora, our research team reported on the antisickling activity of a number of plants used in traditional medicine against SCD in Democratic Republic of Congo (DR Congo) and identified anthocyanins as the main bioactive secondary metabolites class [3,[5][6][7][13][14][15][16][17][18][19][20][21]. A recent conducted survey revealed the use by Congolese traditional practitioners of aqueous extracts of Dicliptera colorata C. B. Clarke, Sorghum bicolor (L.) Moench and Euphorbia hirta L., plants which have not yet been scientifically investigated for their antisickling potent.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Sickling Inhibitory Effects and Anti-Sickle Erythrocytes Hemolysis of Dicliptera colorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench

Mpiana¹,

Lombe²,

Ombeni³

et al. 2013

OJBD

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Diclipteracolorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench are reported among plant species used in Congolese traditional medicine to treat Sickle Cell Disease. These medicinal plants have been investigated for their inhibitory effect on the induced sickling process of red blood cells using Emmel's test. Aqueous plant extracts showed good antisickling activity as revealed by the observed normal biconcave form of sickle red blood cells in anoxic conditions. The calculated radius of treated red blood cells by Euphorbia hirta L., Diclipteracolorata C. B. Clarke and Sorghum biocolor (L.) Moench extracts are 3.31 ± 0.55; 3.34 ± 0.53; 3.17 ± 0.52, respectively. Euphorbia hirta L. is the most active with a normalization rate, more than 70%. The chemical screening performed on these plants showed the presence of anthocyanins which were then extracted. The test carried out with anthocyanin extracts showed that these phenolic compounds have a good antisickling activity and, additionally, possess an anti-hemolytic effect on SS blood. This suggests that the evidenced biological activity of these plants would be due to anthocyanins. The results hence obtained justify the use of these plants in Congolese traditional medicine.

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Activité antidrépanocytaire et thermodégradation des anthocyanes extraits de Sterculia quinqueloba et Ficus capensis

Cited by 8 publications

References 11 publications

Evaluation of the anti-sickling activity of <i>Newbouldia laevis</i> P. Beauv extracts

Evaluation of the anti-sickling activity of <i>Newbouldia laevis</i> P. Beauv extracts

Ethnopharmacological Survey and Antisickling Activity of Plants Used in the Management of Sickle Cell Disease in Kikwit City, DR Congo

<i>In Vitro</i> Sickling Inhibitory Effects and Anti-Sickle Erythrocytes Hemolysis of <i>Dicliptera colorata</i> C. B. Clarke, <i>Euphorbia hirta</i> L. and <i>Sorghum bicolor</i> (L.) Moench

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Activité antidrépanocytaire et thermodégradation des anthocyanes extraits de Sterculia quinqueloba et Ficus capensis

Cited by 8 publications

References 11 publications

Evaluation of the anti-sickling activity of <i>Newbouldia laevis</i> P. Beauv extracts

Evaluation of the anti-sickling activity of <i>Newbouldia laevis</i> P. Beauv extracts

Ethnopharmacological Survey and Antisickling Activity of Plants Used in the Management of Sickle Cell Disease in Kikwit City, DR Congo

&lt;i&gt;In Vitro&lt;/i&gt; Sickling Inhibitory Effects and Anti-Sickle Erythrocytes Hemolysis of &lt;i&gt;Dicliptera colorata&lt;/i&gt; C. B. Clarke, &lt;i&gt;Euphorbia hirta&lt;/i&gt; L. and &lt;i&gt;Sorghum bicolor&lt;/i&gt; (L.) Moench

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<i>In Vitro</i> Sickling Inhibitory Effects and Anti-Sickle Erythrocytes Hemolysis of <i>Dicliptera colorata</i> C. B. Clarke, <i>Euphorbia hirta</i> L. and <i>Sorghum bicolor</i> (L.) Moench