Evaluation of the anti-sickling activity of <i>Newbouldia laevis</i> P. Beauv extracts

Abstract

Sickle cell disease is a genetic disease very expanded all over the African block. In Togo like most African countries, it's one of major causes of morbidity and mortality. The available treatments are very ornamental and are most of the time remedy than cure. However, in our areas, the sicks use medicinal plants for treating themselves. It's in this scope that this study has been conducted in sight of assessing the anti-sickling activity of Newbouldia laevis extracts (leaves, roots and stem barks) based on Emmel test using 2% sodium metabisulphite. The extracts were also studied for its in vitro antioxidant activity using 2,2-diphenyl-1picrylhydrazyl (DPPH) and 2,2'-azobis (2-amidinopropane hydrochloride) (AAPH) methods. The results obtained show that the roots and the stem barks extracts have a comparable efficiency. These extracts enabled us to reduce the sickling rate respectively at 17% and 16% against 78% for the control that was incubated in the presence of 0.9% sodium chloride. The leaves extract led to hemolysis and hemolysing concentration was 5mg/ml. Light antioxidant activity in vitro was obtained for the two extracts. Our outcomes proved that in vitro, roots and stem barks of Newbouldia laevis extracts have an anti-sickling activity contrary to the hemolysing leaves extract.

Antioxidant and Anti-Sickling Activity of Extracts From the Stem and Roots Barks of Strychnos Innocua (Del.)

“…These compounds belong to the polyphenol family. Polyphenols are recognised as being responsible for anti-sickle cell activity by several authors [26,27]. According to Ghosh et al [28], flavonoids improve endothelial function by activating the synthesis of vasorelaxant nitric oxide, which could therefore play an important role in the treatment of sickle cell disease.…”

Antisickling Studies of the Aqueous Leaf Extract of Detarium microcarpum (Tallow Tree)

“…The quantitative phytochemical assay revealed the presence of 31.3 mg/g flavonoids in the extract. Due to the antioxidant activity of flavonoids, studies have shown that they play a significant role in the prevention and management of many chronic diseases including sickle cell diseases (John et al, 2018;Dermane et al, 2018). Thus, the observed antisickling activity may be due to the abundant flavonoids present in the extract.…”

Nutritional and Endophytic Composition of Edible Tubers of Tiger nut (Cyperus esculentus L.)

“…Phytochemicals reported in this study have different pharmacological properties, hence supporting the nutritional and medicinal values of tiger nut tubers. The phytochemicals have hepatoprotective, anti-sickling, aphrodisiac, antimicrobial, anti-inflammatory, and antioxidant properties (Da Silva et al, 2012;Rehman et al, 2015;Dermane et al, 2018;Yang et al, 2020). Furthermore, anthroquinone, a phytochemical compound known to have a laxative effect and relevant in the cure for constipation (Lombardi et al, 2020), was identified, in tiger nut samples purchased from the Ado-Ekiti market.…”