Objective: This study aims to evaluate the fractions from whole extracts of roots and stem barks of Newbouldia laevis and their effect on sickling.
Methods: Hydroethanolic extracts of stem barks and roots of Newbouldia laevis were fractionated by the technics of cold precipitation in ethanol. The fractions obtained after phytochemical screening were subjected to Emmel test to evaluate their anti-sickling activity. Active fractions were tested for DPPH and AAPH assay (AAPH induced membrane lipoperoxidation and evaluation of reduction of hemolysis).
Results: Two fractions were obtained from each whole extract: supernatant and pellet fractions. Supernatants fractions obtained from whole roots barks extract and stem barks extract at a concentration of 30 mg/ml reduced sickling up respectively to 7% and 10% against 86% for the control. Pellets fractions obtained from the both extracts induced coagulation of SS blood at 30 mg/ml against 86% for the control.
Conclusion: Supernatants fractions of hydroethanolic whole extract of roots and stem backs of Newbouldia laevis promise as the potential source of active molecules against sickle cell disease.
Sickle cell disease is a genetic disease very expanded all over the African block. In Togo like most African countries, it's one of major causes of morbidity and mortality. The available treatments are very ornamental and are most of the time remedy than cure. However, in our areas, the sicks use medicinal plants for treating themselves. It's in this scope that this study has been conducted in sight of assessing the anti-sickling activity of Newbouldia laevis extracts (leaves, roots and stem barks) based on Emmel test using 2% sodium metabisulphite. The extracts were also studied for its in vitro antioxidant activity using 2,2-diphenyl-1picrylhydrazyl (DPPH) and 2,2'-azobis (2-amidinopropane hydrochloride) (AAPH) methods. The results obtained show that the roots and the stem barks extracts have a comparable efficiency. These extracts enabled us to reduce the sickling rate respectively at 17% and 16% against 78% for the control that was incubated in the presence of 0.9% sodium chloride. The leaves extract led to hemolysis and hemolysing concentration was 5mg/ml. Light antioxidant activity in vitro was obtained for the two extracts. Our outcomes proved that in vitro, roots and stem barks of Newbouldia laevis extracts have an anti-sickling activity contrary to the hemolysing leaves extract.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.