<i>In Vitro</i> Sickling Inhibitory Effects and Anti-Sickle Erythrocytes Hemolysis of <i>Dicliptera colorata</i> C. B. Clarke, <i>Euphorbia hirta</i> L. and <i>Sorghum bicolor</i> (L.) Moench

Abstract

Diclipteracolorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench are reported among plant species used in Congolese traditional medicine to treat Sickle Cell Disease. These medicinal plants have been investigated for their inhibitory effect on the induced sickling process of red blood cells using Emmel's test. Aqueous plant extracts showed good antisickling activity as revealed by the observed normal biconcave form of sickle red blood cells in anoxic conditions. The calculated radius of treated red blood cells by Euphorbia hirta L., Diclipteracolorata C. B. Clarke and Sorghum biocolor (L.) Moench extracts are 3.31 ± 0.55; 3.34 ± 0.53; 3.17 ± 0.52, respectively. Euphorbia hirta L. is the most active with a normalization rate, more than 70%. The chemical screening performed on these plants showed the presence of anthocyanins which were then extracted. The test carried out with anthocyanin extracts showed that these phenolic compounds have a good antisickling activity and, additionally, possess an anti-hemolytic effect on SS blood. This suggests that the evidenced biological activity of these plants would be due to anthocyanins. The results hence obtained justify the use of these plants in Congolese traditional medicine.

Ethno-Pharmacological Survey, In Vitro Anti-Sickling and Free Radical Scavenging Activities of Carapa Procera DC. Stem Bark (Meliaceae)

“…A similar result was already obtained for some medicinal plant species used for the management of SCD by Congolese traditional healers. This activity could be due to compounds easily extracted by the used polar solvents such as anthocyanins or to phenolic or triterpenoic acids as previously reported [13][14][15][16][17][18][19][20]23]. The treated SS RBCs demonstrated a remarkable similarity to normal blood values.…”

“…Fractions were filtered and concentrated to dryness under reduced pressure using a rotary evaporator. Extraction of anthocyanins was then done using 100 g of dried powdered plant material with acidified methanol (1% HCl) following an established protocol [13][14][15][16][17][18][19][20]. Anthocyanins extract was then defatted by n-hexane.…”

“…Herbal extracts have been used in African folk medicine for decade in the management of various ailments [9][10][11][12]. Recently our research team shows that many medicinal plants used in traditional medicine in DRC to manage SCD had in vitro antisickling activity and that this activity is mainly due to anthocyanins [13][14][15][16][17][18][19][20]. As several other flavonoids, anthocyanins are natural products with a range of biological activities including free radical scavengers and show antioxidant activity [21,22].…”

Preliminary study to identify anti-sickle cell plants in Nigers traditional pharmacopoeia and their phytochemicals

“…The anthocyanin antioxidant efficacy can also be responsible for reduction of haemolysis of erythrocytes. Furthermore, compound with potent antioxidant efficacy and thus capable of preventing generation of free radicals (Mpiana et al, 2013) could be used as an antisickling drug. Bandara et al (2018) revealed the presence of anthocyanin in the aqueous extract of Passiflora suberosa with potent antihaemolytic activity.…”

Chemical Fingerprint and Anti-Sickling Activity of Rosmarinic Acid and Methanolic Extracts from Three Species of <i>Ocimum</i> from DR Congo

“…This chronic disease affects mainly sub-Saharan African. In Democratic Republic of the Congo (RDC) more than one and half millions of people are affected [3]- [5].…”