Acrylic-based genosensor utilizing metal salphen labeling approach for reflectometric dengue virus detection

Abstract

An optical genosensor based on Schiff base complex (Zn2+ salphen) DNA label and acrylic microspheres (AMs) as polymer support of the capturing DNA probe (cpDNA) was developed for dengue virus serotype 2 (DEN-2) detection via reflectance spectrophotometric method. The solid-state optical DNA biosensor showed high selectivity and specificity up to one-base mismatch in the target DNA sequence owing to the salphen chemical structure that is rich in localized electrons, and allowed π-π stacking interaction between stacked base pairs of doublestranded DNA (dsDNA). The reflectometric DNA microsensor demonstrated a broad linear detection range towards DEN-2 DNA from 1 × 10-15 M to 1 × 10−3 M with a low limit of detection (LOD) obtained at 1.21 × 10-16 M. The DNA biosensor gave reproducible optical response with a satisfactory relative standard deviation (RSD) at 3.1%, (n = 3), and the reflectance response was stable even after four regeneration cycles of the DNA biosensor. The optical genosensor was proven comparable with standard reverse transcription polymerase chain reaction (RT-PCR) in detecting DEN-2 genome acquired from clinical samples of serum, urine and saliva of dengue virus infected patients under informed consent. The developed reflectometric DNA biosensor is advantageous in offering an early DEN-2 diagnosis, when fever symptom started to manifest in patient.

Synthesis of a Novel Zn-Salphen Building Block and Its Acrylic Terpolymer Counterparts as Tunable Supramolecular Recognition Systems

“…However, the use of Zn-Salphen building blocks as a broadly applicable strategy for developing molecularly tunable materials in polymer science is still in its early stages [29]. In the literature, these building blocks have been incorporated into polymeric systems using two main strategies: supramolecular functionalization to obtain Zn-Salphen-composites [30,31,32] and homo-polymerization to produce covalently linked Zn-Salphen oligomers [33,34]. Nevertheless, to date, their potential as a supramolecular tool for tailoring the functional properties of more complex co-polymer materials has remained underexploited.…”

Strategies for the preparation of non-amplified and amplified genomic dengue gene samples for electrochemical DNA biosensing applications

“…Due to all these circumstances, researchers have recently focused on the development of point-of-care quantitative biosensors for dengue virus diagnosis with high selectivity and specicity, portability, and ease of use. [2][3][4][5] In terms of sensor biorecognition elements, nucleic acid or DNA biosensors are preferred over enzyme-, antibody-, or microorganism-based biosensors due to their specic binding through DNA hybridization events, high stability, ease of synthesis, minimal batch-to-batch variation, and biocompatibility. 6 The underlying principle behind the mechanism of a DNA biosensor is based on the detection of a DNA hybridization event, which is the process of joining two single-stranded DNA (ssDNA) strands between immobilized ssDNA probes with the formation of target double-stranded complementary DNA (dsDNA).…”