Aclimatação de gengibre (Zingiber officinale Roscoe) e a relação com carboidratos endógenos

Girardi, Carla Giovana; Pescador, Rosete

doi:10.1590/s1516-05722010000100010

Cited by 11 publications

(4 citation statements)

References 23 publications

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“…According to Girardi & Pescador (2010), another limiting factor in acclimatization is the adaptation of the plants from in vitro conditions, after transferring to environmental conditions ex vitro. This method sometimes entails low survival rates of the seedlings as a consequence of low photosynthetic rates of plants that are not completely autotrophic.…”

Section: Resultsmentioning

confidence: 99%

In vitro propagation and seedling acclimatization of Caesalpinia ferrea Mart., a valuable medicinal plant in the Amazon (Fabaceae)

et al. 2020

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The objective of this work was to establish a protocol for the rapid in vitro multiplication of Caesalpinia ferrea Mart., aimed at genetic selection and in vitro germplasm conservation. Seeds treated in different sodium hypochlorite solution concentrations were inoculated in Murashige and Skoog (MS) culture medium and maintained in a growth room. After 30 days, sodium hypochlorite at 0.25% concentration resulted in 96.67% survival and 90% germination of the seeds. In the explants multiplication phase three types of medium culture were evaluated supplemented with cytokinins and auxins in different concentrations and combinations. The addiction of 0.1 mg·L-1 Benzylaminopurine (BAP) and 0.1 mg·L-1 Indole butyric acid (IBA) induced 100% of explants with shoots and 32% with root formation, while a complete absence of callus was found. It was observed that 90% of explants derived from the buds developed shoots with 2.73 cm and greater multiplication rate (10.06). The seedlings obtained from in vitro multiplication were acclimated without the presence of roots in different types of substrate. After 60 days, soil substrate was most indicated for acclimatization, showing 63.33% of healthy and uniform plants. In vitro propagation of C. ferrea allows the establishment and multiplication of in vitro seedlings from nodal segments and axillary buds.

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Section: Resultsmentioning

confidence: 99%

In vitro propagation and seedling acclimatization of Caesalpinia ferrea Mart., a valuable medicinal plant in the Amazon (Fabaceae)

et al. 2020

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“…The acclimatization is also influenced by the substrate used, which may facilitate or impede seedling growth according to their physicochemical properties. The material used as substrate must be inert, porous, well-drained, and able to maintain aeration and humidity, thus allowing root system development (Girardi & Pescador, 2010;Hoffmann et al, 2001;Vasconcelos et al, 2015). While the substrate may be formed of a single material, it will be difficult to find one able to provide all the necessary characteristics, and for this reason substrates generally represent the blending of two or more materials to optimize usage conditions.…”

Section: First Acclimatization Period In Vitro (Five Days In the Culture Room With Covered Plastic Cups)mentioning

confidence: 99%

Sucrose and substrates on the acclimatization of micropropagated Luehea divaricata plants

et al. 2020

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“…Similar results were also reported by Oliveira et al (2010) during the acclimatization of Z. spectabile, with the use of other substrates and inoculated with fungus arbuscular mycorrhizal fungi. Others species of Zingiberales (Etlingera elatior, Zingiber officinal, Heliconia velloziana) also presented great survival to acclimatization process (GIRARDI and PESCADOR, 2010;DAS et al, 2013;QUISEN et al, 2014;RODRIGUES et al, 2015).…”

Section: Acclimatizationmentioning

confidence: 99%

Propagação in vitro de Zingiber spectabile

Reis

Nery²,

Prudente³

et al. 2017

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Zingiber spectabile is a tropical ornamental species with difficulties to obtain efficient propagation system. Thus, this study aimed to assess the in vitro propagation of Zingiber spectabile. Seed characterization was determined by measuring length, width and thickness, the weight of 1000 seeds and imbibition curve. In vitro germination of seeds was at constant (25 °C) or alternating temperatures (20-30 ºC). For optimization of in vitro multiplication, different concentrations of activated charcoal (0.0, 0.1 and 0.3%) and sucrose (0.0, 0.1, 0.3, 0.5 and 0.7 M) were evaluated. Plantlets were inoculated in flasks with different sealing systems (PVC covers with or without filters at the center) and culture media (MS or WPM). The plants were acclimatized in Plantmax ® substrate. Seeds were of 6.06 mm length, 3.22 mm wide and 2.83 mm thick. The weight of 1,000 seeds corresponded to 46.4 g. The seed imbibition curve approaches to a tree phase pattern. Alternating temperatures induced high germination rates (68%). The addition of 0.3% activated charcoal provided higher root growth and plants with smaller number of senescent leaves. The best plant growth was obtained by the use of 0.1 M sucrose. All acclimatized plants survived (100%). The results demonstrate that Z. spectabile respond well to in vitro propagation. Keywords: beehive ginger, micropropagation, tropical plants. RESUMO Propagação in vitro de Zingiber spectabileZingiber spectabile é uma espécie ornamental tropical com dificuldades para se obter um sistema de propagação eficiente. Assim, este estudo teve como objetivo avaliar a propagação in vitro de Z. spectabile. A caracterização das sementes foi determinada pela medição do comprimento, largura e espessura, pelo peso de 1.000 sementes e pela curva de embebição. A germinação in vitro das sementes foi avaliada em temperatura constante (25 °C) ou alternada (20-30 ºC). Para a otimização da multiplicação in vitro, diferentes concentrações de carvão ativado (0,0, 0,1 e 0,3%) e sacarose (0,0, 0,1, 0,3, 0,5 e 0,7 M) foram avaliadas. As plântulas foram inoculadas em frascos com diferentes sistemas de vedação (tampas de PVC com ou sem filtros no centro) e meio de cultura (MS ou WPM). As plantas foram aclimatizadas em substrato Plantmax®. As sementes apresentaram comprimento de 6,06 mm, largura de 3,22 cm e espessura de 2,83 mm. O peso de 1000 sementes correspondeu a 46,4 g. A curva de embebição de sementes se aproxima de um padrão trifásico. Temperaturas alternadas induziram altas taxas de germinação (68%). A adição de 0,3% de carvão ativado proporcionou maior crescimento radicular e plantas com menor número de folhas senescentes. O melhor crescimento de plantas foi obtido pelo uso de sacarose 0,1 M. Todas as plantas aclimatizadas sobreviveram (100%). Os resultados demonstraram que Z. spectabile responde bem à propagação in vitro. Palavras-chave: sorvetão, micropropagação, plantas tropicais.

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Aclimatação de gengibre (Zingiber officinale Roscoe) e a relação com carboidratos endógenos

Cited by 11 publications

References 23 publications

In vitro propagation and seedling acclimatization of Caesalpinia ferrea Mart., a valuable medicinal plant in the Amazon (Fabaceae)

In vitro propagation and seedling acclimatization of Caesalpinia ferrea Mart., a valuable medicinal plant in the Amazon (Fabaceae)

Sucrose and substrates on the acclimatization of micropropagated Luehea divaricata plants

Propagação in vitro de Zingiber spectabile

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