Sucrose and substrates on the acclimatization of micropropagated Luehea divaricata plants

Silva, Karol Buuron da; Reiniger, Lia Rejane Silveira; Stefanel, Charlene Moro; Rabaiolli, Silvia Machado dos Santos

doi:10.1590/2179-8087.117017

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…In addition, it should facilitate the acquisition of water and nutrients and allow gas exchange. This will promote plant growth and survival and the development of a good root system [62].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Root Induction from Argan (Argania spinosa (L.) Skeels) Adventitious Shoots: Influence of Ammonium Nitrate, Auxins, Silver Nitrate and Putrescine, and Evaluation of Plantlet Acclimatization

Amghar

Ibriz

Ibrahimi

et al. 2021

Plants

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Argania spinosa (L.) Skeels is an endangered plant species endemic to Morocco. In recent years, attempts to develop in vitro regeneration systems for this species were made. However, rooting and acclimatization of in vitro plants have been a bottleneck for successful propagation. In the present study, the effects of different concentrations of auxins, putrescine, silver nitrate (AgNO3) and ammonium nitrate on the in vitro rooting of adventitious shoots of two argan genotypes “Mejji” and “R’zwa”, were evaluated. The highest rooting percentages (86.6% in “Mejji” and 84.4% in “R’zwa”) were observed on Murashige and Skoog (MS) medium modified by reducing the ammonium nitrate concentration and supplemented with 1.5 mg L−1 indole-3-butyric acid (IBA), 0.5 mg L−1 1-naphthalene acetic acid (NAA), 2 mg L−1 AgNO3 and 160 mg L−1 putrescine. This medium resulted in the development of a good root system after only 10 days of culture. Plantlet acclimatization was carried out using different substrate mixtures, and high survival rates (100%) were observed when the substrate contained either peat alone or a sand–peat mixture (1:1, w/w). The high percentages of rooting and acclimatization reported in the present study are of high importance for rapid and large-scale propagation of this endangered species.

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Section: Discussionmentioning

confidence: 99%

In Vitro Root Induction from Argan (Argania spinosa (L.) Skeels) Adventitious Shoots: Influence of Ammonium Nitrate, Auxins, Silver Nitrate and Putrescine, and Evaluation of Plantlet Acclimatization

Amghar

Ibriz

Ibrahimi

et al. 2021

Plants

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“…A micropropagação surge como uma alternativa tanto para a multiplicação dos genótipos de interesse, quanto para a conservação de recursos genéticos (Flôres et al, 2011;Silva et al, 2019Silva et al, , 2020. Essa técnica da cultura de tecidos permite obter, simultaneamente, progressos no melhoramento de plantas por meio da propagação vegetativa, com uma menor incidência de doenças e independente do clima e da época do ano (Fermino Júnior & Scherwinski-Pereira, 2012;Oliveira et al, 2013), bem como a conservação de recursos genéticos florestais, pela germinação in vitro de sementes.…”

Section: Introductionunclassified

Efeito de diferentes períodos de cultivo na micropropagação de brotações de Luehea divaricata

et al. 2021

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O objetivo do presente trabalho foi avaliar o efeito do período de cultivo na micropropagação de brotações de Luehea divaricata. Os tratamentos foram compostos pelos períodos de cultivo in vitro: 14; 21; 28; 40; 56; 70; 84; 102; 116; 130; 154, 186 ou 200 dias, sem realizar subcultivo ou transferência para outro meio nutritivo fresco. A sobrevivência e o estabelecimento ficaram em torno de 90% até 84 dias de cultivo. A partir deste período, a mortalidade foi mais acentuada. Para as variáveis número total de folhas e folhas com senescência, o período de cultivo de 56 dias foi o que mostrou uma proporção mais adequada, em que para cada nove folhas formadas apenas uma estava senescente. Em relação à formação de raízes primárias, médias superiores a 50% foram observadas após 70 dias de cultivo in vitro, chegando aos melhores resultados (60%) aos 200 dias. Para raízes secundárias, as maiores médias foram observadas após 102 dias (41,5%), alcançando 45,3% aos 200 dias. A micropropagação de Luehea divaricata é afetada significativamente pelo período de cultivo in vitro, que pode ser estendido até aproximadamente 60 dias. A partir desse período, são registradas altas mortalidades.

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Effect of selected substrates and chitosan on growth performance of orchid tissue culture seedling under net house

Rahman

Bakar

Kayat

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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Orchids are considered as the most beautiful and significant cut blossom and pruned plants throughout the world. Therefore tissue culture technique was used as one of the alternative to mass propagate the orchids to fulfil the demand. This study was conducted to find out the effect of chitosan and different substrates on growth performance of Dendrobium Shavin White orchid seedling from tissue culture environment to net house. The tissue cultured plants went through prehardening stage to reduce mortality after the switch to ex vitro conditions. Five (5) treatments consist of combination substrates were tested which were T1: rice husk + chitosan, T2: peat moss + chitosan, T3: EFB + chitosan, T4: rice husk + peat moss + chitosan and T5: rice husk + peat moss + EFB + chitosan whereas charcoal as a control (T0). The result showed that the highest plant height and leaf length which did not significantly different were T3 (6.20cm plant height, 4.83cm leaf length), and T5 (6.55cm plant height, 4.51cm leaf length). Meanwhile, T3 was an effective for number of leaf production with 4.14 leaf count. Hence, this data will be used to acclimatize tissue cultured of Dendrobium Shavin White orchid for commercial used.

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Sucrose and substrates on the acclimatization of micropropagated Luehea divaricata plants

Cited by 4 publications

References 19 publications

In Vitro Root Induction from Argan (Argania spinosa (L.) Skeels) Adventitious Shoots: Influence of Ammonium Nitrate, Auxins, Silver Nitrate and Putrescine, and Evaluation of Plantlet Acclimatization

In Vitro Root Induction from Argan (Argania spinosa (L.) Skeels) Adventitious Shoots: Influence of Ammonium Nitrate, Auxins, Silver Nitrate and Putrescine, and Evaluation of Plantlet Acclimatization

Efeito de diferentes períodos de cultivo na micropropagação de brotações de Luehea divaricata

Effect of selected substrates and chitosan on growth performance of orchid tissue culture seedling under net house

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