Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles

Chiang, Cheng Kang; Lin, Yu‐Liang; Chen, Wen Tsen; Chang, Huan‐Tsung

doi:10.1016/j.nano.2010.01.006

Cited by 57 publications

(36 citation statements)

References 24 publications

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“…By using CAP as an internal standard, this SALDI-MS provided spot-to-spot and batch-to-batch variations of less than 10% and 13%, respectively, for the analytes at 10 μM. Minimum problems associated with sweet spots when using inorganic matrices have been reported [33,34]. …”

Section: Sensitivity and Linearitymentioning

confidence: 95%

Tea Identification through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry

Chen¹

2013

IJAMSC

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We have applied surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using titanium dioxide nanoparticles (TiO 2 NPs) as the matrix and captopril (CAP) as internal standard for the determination of the concentrations of theanine and four catechins-catechin, ( _ )-epigallocatechin (EGC), ( _ )-epicatechin gallate (ECG), and ( _ )-epigallocatechin gallate (EGCG). Under the optimal conditions (240 nM TiO 2 NPs and 10 μM CAP), this SALDI-MS approach provides linearity of 0.3 -80 (r = 0.990), 1.2 -100 (r = 0.987), 4 -120 (r = 0.995), 6 -120 (r = 0.983), and 2 -120 μM (r = 0.991) for theanine, catechin, EGC, ECG, and EGCG, respectively. The limits of detection (LODs; S/N = 3) for theanine, catechin, EGC, ECG, and EGCG provided by this SALDI-MS approach are 0.1, 0.35, 1.0, 1.45, and 0.5 μM, respectively. This approach provides spot-to-spot and batch-to-batch variations of less than 10% and 13%, respectively, for the analysis of tea samples. With advantages of simplicity, accuracy, precision, and great reproducibility, we have applied the SALDI-MS approach for the analysis of tea samples, with identified peaks for theanine, catechin, EGC, ECG, and EGCG. Tea samples from Taiwan and four other areas have various SALDI-MS profiles, showing their potential for differentiation of tea samples from different sources. Our result also shows that tea samples harvested in different seasons and counties in Taiwan provide significantly different MS profiles. The amounts of theanine and EGC in the Oolong tea from Lishan are much higher than those in the other tea samples.

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Section: Sensitivity and Linearitymentioning

confidence: 95%

Tea Identification through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry

Chen¹

2013

IJAMSC

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“…Therefore, internal standards are commonly used in MS to improve the accuracy when determining the unknown concentrations of analytes [31,51]. We first compared the quantitative analysis of 5-HIAA with and without the internal standard of NAD by SALDI-TOF MS.…”

Section: Quantitative Analyses Of 5-hiaa Trp 5-htp and 5-ht By Salmentioning

confidence: 99%

“…Previous studies have reported that gold nanoparticles were used as matrixes for the detection of organic compounds, peptides, and drugs with remarkable sensitivities [29][30][31]. The implementation of gold nanoparticles as the matrix was revealed to be an important method for desorption and ionization.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of multiple urinary biomarkers of carcinoid tumors through gold-nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry

Kuo

Chen

Chiu

et al. 2011

Analytica Chimica Acta

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“…Although the accurate advanced instrumental techniques can be used for the analysis of glutathione oxidative stress biomarker (Chiang et al, 2010), there is a need for the development of inexpensive, field-deployable analytical platforms for disease screening and protection against environmental exposure (Noble et al, 2005), (James et al, 2006). The application of microsensors for screening of biomarkers of oxidative stress has been explored.…”

Section: Microsensor Arraysmentioning

confidence: 99%

Detection of Oxidative Stress Biomarkers Using Novel Nanostructured Biosensors

Hepel¹,

Stobiecka²

2011

New Perspectives in Biosensors Technology and Applications

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Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles

Cited by 57 publications

References 24 publications

Tea Identification through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry

Tea Identification through Surface-Assisted Laser Desorption/Ionization Mass Spectrometry

Quantitative analysis of multiple urinary biomarkers of carcinoid tumors through gold-nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry

Detection of Oxidative Stress Biomarkers Using Novel Nanostructured Biosensors

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