Quantitative analysis of multiple urinary biomarkers of carcinoid tumors through gold-nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry

Kuo, Tsung‐Rong; Chen, Jinn Shiun; Chiu, Yi-Wei; Tsai, Chia Yi; Hu, Cho Chun; Chen, Chia Chun

doi:10.1016/j.aca.2011.05.012

Cited by 22 publications

(10 citation statements)

References 52 publications

(52 reference statements)

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“…The high surface area of nanoparticles allows better sample deposition and absorption and homogeneity in the mixture, preventing formation of sweet spots upon application [27,78,79]. What is more, spot-to-spot and shot-to-shot variability is considerably diminished for more accurate quantification approaches [14,28]. In contrast to what happens in commonly used organic matrices, NPs in general are not so easily ionizable upon laser irradiation [29][30][31].…”

Section: Nanoparticle-based Mass Spectrometry Detectionmentioning

confidence: 92%

“…These compounds interact weakly with AuNPs, through Au-N bonds, and allow easy and rapid separation through centrifugation, prior to analysis. On a different study, AuNP-based SALDI was employed toward quantification of four urinary biomarkers for carcinoid tumors in human urine samples [28], by using an internal standard. Recently, a nucleotide-fingerprint identification concept was proposed for the determination of modified and unmodified nucleotides using AuNPs for SALDI-TOF [38].…”

Section: Nanoparticles For Laser Desorption/ionization Mass Spectromementioning

confidence: 99%

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Nanoparticles for Mass Spectrometry Applications

Larguinho

Capelo

Baptista

2015

Handbook of Nanoparticles

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Nanotechnology has led to the development of new and improved materials, and particular emphasis has been directed toward nanoparticles and their multiple bio-applications. Nanoparticles exhibit size-, shape-, and composition-dependent properties, e.g., surface plasmon resonance and photothermal properties, which may potentially enhance laser desorption/ionization systems for mass spectrometry-based analysis of biomolecules. Also, nanoparticles possess high surface to volume ratio that can be easily derivatized with a wide range of ligands with different functional groups. Surface modification makes nanoparticles advantageous for sample preparation procedures prior to detection by mass spectrometry. Moreover, it allows the synthesis of affinity probes, which promotes interactions between nanoparticles and analytes, greatly enhancing the ionization efficiency.This chapter provides a comprehensive discussion on the use of nanoparticles for mass spectrometryrelated applications, from sample preparation methodologies to ionization surfaces. Applications will focus on nanoparticle size, composition, and functionalization, as a comparative point of view on optimal characteristics toward maximization of bioassay efficiency.

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Section: Nanoparticle-based Mass Spectrometry Detectionmentioning

confidence: 92%

Section: Nanoparticles For Laser Desorption/ionization Mass Spectromementioning

confidence: 99%

Nanoparticles for Mass Spectrometry Applications

Larguinho

Capelo

Baptista

2015

Handbook of Nanoparticles

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“…Carbon materials including porous oxidized graphitized carbon black powder [16], multi-walled carbon nanotubes [17] and graphitic carbon nitride nanosheets [18], which possess an absorption capacity in the wide electromagnetic spectrum, have been used in the quantitative SALDI analysis of small drugs or small organics. Au NPs [19][20][21][22][23][24] are widely used in quantitative SALDI-MS analysis. In addition to ease of preparation and functionalization, Au NPs also possess a desirable absorption capacity in the ultraviolet (UV) region, which the wavelength of the laser used in SALDI-MS belongs to.…”

Section: (B) Inorganic Nanoparticles Used In Quantitative Surface-assmentioning

confidence: 99%

“…Au NP-based SALDI-MS has been applied in the quantitative analysis [19][20][21][22][23][24] of small organics, small biomolecules and drugs. The particle size of the Au NPs used in most of these studies is in the range of 10-20 nm [20][21][22][23]. Au NPs with particle sizes of less than 10 nm [19] and greater than 20 nm [24] used as SALDI-assisting materials have also reported.…”

Section: (B) Inorganic Nanoparticles Used In Quantitative Surface-assmentioning

confidence: 99%

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Revisiting the quantitative features of surface-assisted laser desorption/ionization mass spectrometric analysis

Wu¹,

Lee²,

Kuo³

et al. 2016

Phil. Trans. R. Soc. A.

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Surface-assisted laser desorption/ionization (SALDI) coupled with mass spectrometry (MS) is frequently used to analyse small organics owing to its clean background. Inorganic materials can be used as energy absorbers and the transfer medium to facilitate the desorption/ionization of analytes; thus, they are used as SALDI-assisting materials. Many studies have demonstrated the usefulness of SALDI-MS in quantitative analysis of small organics. However, some characteristics occurring in SALDI-MS require certain attention to ensure the reliability of the quantitative analysis results. The appearance of a coffee-ring effect in SALDI sample preparation is the primary factor that can affect quantitative SALDI-MS analysis results. However, to the best of our knowledge, there are no reports relating to quantitative SALDI-MS analysis that discuss or consider this effect. In this study, the coffee-ring effect is discussed using nanoparticles and nanostructured substrates as SALDI-assisting materials to show how this effect influences SALDI-MS analysis results. Potential solutions for overcoming the existing problems are also suggested.This article is part of the themed issue 'Quantitative mass spectrometry'.

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