Detection of Oxidative Stress Biomarkers Using Novel Nanostructured Biosensors

Hepel, Maria; Stobiecka, Magdalena

doi:10.5772/17916

Cited by 3 publications

(2 citation statements)

References 120 publications

(119 reference statements)

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“…The CDD system may also serve as an efficient vehicle to overcome MDR [ 6 , 7 , 10 ], which is challenging due to the involvement of alterations in several signaling pathways. MDR is acquired by tumors as a protection against reactive oxygen species (ROS), the main weapon of the organism to fight cancer [ 22 , 23 , 24 , 25 ], by overexpressing anti-apoptotic proteins and glutathione (GSH) [ 26 , 27 ], able to neutralize highly active radicals of ROS. Due to MDR, an increased dose of chemotherapeutic agents is necessary to overcome drug resistance, inevitably leading to increased collateral damage to healthy cells.…”

Section: Introductionmentioning

confidence: 99%

Cancer-Targeted Controlled Delivery of Chemotherapeutic Anthracycline Derivatives Using Apoferritin Nanocage Carriers

Kurzątkowska

Pazos

Herschkowitz

et al. 2021

IJMS

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The interactions of chemotherapeutic drugs with nanocage protein apoferritin (APO) are the key features in the effective encapsulation and release of highly toxic drugs in APO-based controlled drug delivery systems. The encapsulation enables mitigating the drugs’ side effects, collateral damage to healthy cells, and adverse immune reactions. Herein, the interactions of anthracycline drugs with APO were studied to assess the effect of drug lipophilicity on their encapsulation excess n and in vitro activity. Anthracycline drugs, including doxorubicin (DOX), epirubicin (EPI), daunorubicin (DAU), and idarubicin (IDA), with lipophilicity P from 0.8 to 15, were investigated. We have found that in addition to hydrogen-bonded supramolecular ensemble formation with n = 24, there are two other competing contributions that enable increasing n under strong polar interactions (APO(DOX)) or under strong hydrophobic interactions (APO(IDA) of the highest efficacy). The encapsulation/release processes were investigated using UV-Vis, fluorescence, circular dichroism, and FTIR spectroscopies. The in vitro cytotoxicity/growth inhibition tests and flow cytometry corroborate high apoptotic activity of APO(drugs) against targeted MDA-MB-231 adenocarcinoma and HeLa cells, and low activity against healthy MCF10A cells, demonstrating targeting ability of nanodrugs. A model for molecular interactions between anthracyclines and APO nanocarriers was developed, and the relationships derived compared with experimental results.

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Section: Introductionmentioning

confidence: 99%

Cancer-Targeted Controlled Delivery of Chemotherapeutic Anthracycline Derivatives Using Apoferritin Nanocage Carriers

Kurzątkowska

Pazos

Herschkowitz

et al. 2021

IJMS

Self Cite

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show abstract

“…The CDD system may also serve as an efficient vehicle to overcome MDR [6,7,10], which is challenging due to the involvement of alterations in several signaling pathways. MDR is acquired by tumors as a protection against reactive oxygen species (ROS), the main weapon of the organism to fight cancer [22][23][24][25], by overexpressing anti-apoptotic proteins and glutathione (GSH) [26,27], able to neutralize highly active radicals of ROS. Due to MDR, an increased dose of chemotherapeutic agents is necessary to overcome drug resistance, inevitably leading to increased collateral damage to healthy cells.…”

Section: Introductionmentioning

confidence: 99%

Cancer-targeted Controlled Delivery of Chemotherapeutic Anthracycline DerivativesUsing Apoferritin Nanocage Carriers

Kurzątkowska¹,

Pazos²,

Herschkowitz³

et al. 2020

Preprint

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The interactions of chemotherapeutic drugs with nanocage protein apoferritin (APO) are the key features in the effective encapsulation and release of highly toxic drugs in APO-based controlled drug delivery systems. The encapsulation enables mitigating the drugs side effects, collateral damage to healthy cells, and adverse immune reactions. Herein, the interactions of anthracycline drugs with APO were studied to assess the effect of drug lipophilicity on their encapsulation excess n and in vitro activity. Anthracycline drugs, including doxorubicin (DOX), epirubicin (EPI), daunorubicin (DAU), and idarubicin (IDA), with lipophilicity P from 0.8 to 15, were investigated. We have found that in addition to hydrogen-bonded supramolecular ensemble formation with n = 24, there are two other competing contributions that enable increasing n under strong polar interactions (APO(DOX)) or under strong hydrophobic interactions (APO(IDA) of the highest efficacy). The encapsulation/release processes were investigated using UV-Vis, fluorescence, circular dichroism, and FTIR spectroscopies. In vitro cytotoxicity/growth inhibition tests and flow cytometry corroborate high apoptotic activity of APO(drugs) against targeted MDA-MB-231 adenocarcinoma and HeLa cancer cells, and low activity against non-tumorigenic MCF10A cells, demonstrating targeting ability of nanodrugs. A model for molecular interactions between anthracyclines and APO nanocarriers was developed, and the relationships derived compared with experimental results.

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The detection method for small molecules coupled with a molecularly imprinted polymer/quantum dot chip using a home-built optical system

et al. 2016

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A method to detect small molecules with a molecularly imprinted polymer/quantum dot (MIP-QD) chip using a home-built optical fluidic system was first proposed in this study. Ractopamine (RAC) was used as the model molecule to demonstrate its feasibility. The sensing of the target molecule is based on the quenching amount of the quantum dots. The method is facile, cost-saving, easy for miniaturization and avoids the cumbersome steps that are needed to get the fluorescent quenching curve using a spectrofluorometer. Most importantly, more details and accurate response time can be obtained by use of this method. The experimental results show that the prepared chips with low cost are highly selective and the home-built detection system allows the fast binding kinetics. The recorded quenching process was used to study the kinetic uptake of RAC onto the MIP-QD chip and the specificity towards RAC. The system can further be utilized to study the effect of the solvent, pH and temperature on the selectivity of the prepared MIP. The methodology could be extended to other similar studies with different molecules. Graphical abstract Schematic illustration of the molecularly imprinted polymer/quantum dot chip capturing the target molecule.

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Detection of Oxidative Stress Biomarkers Using Novel Nanostructured Biosensors

Cited by 3 publications

References 120 publications

Cancer-Targeted Controlled Delivery of Chemotherapeutic Anthracycline Derivatives Using Apoferritin Nanocage Carriers

Cancer-Targeted Controlled Delivery of Chemotherapeutic Anthracycline Derivatives Using Apoferritin Nanocage Carriers

Cancer-targeted Controlled Delivery of Chemotherapeutic Anthracycline DerivativesUsing Apoferritin Nanocage Carriers

The detection method for small molecules coupled with a molecularly imprinted polymer/quantum dot chip using a home-built optical system

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