Abilities of organic acids to support growth and anthocyanin accumulation by suspension cultures of wild carrot cells using ammonium as the sole nitrogen source

Dougall, Donald K.; Weyrauch, Keith W.

doi:10.1007/bf02619335

Cited by 25 publications

(7 citation statements)

References 14 publications

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“…The correlation shown here between the cessation of anthocyanin accumulation and increasing of medium pH to 5.0-5.2 is supported by the observations that less anthocyanin is accumulated when the pH of the medium is 5.0 or 5.5 compared to pH4.5 in batch cultures [8] and in semicontinuous chemostat cultures [7]. In the medium of these experiments the pH appears to be a result of simultaneous utilization of ammonia (leading to acidification) and of succinate (leading to alkalinization) of the medium.…”

Section: Discussionsupporting

confidence: 67%

Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells

Dougall

Frazier

1989

Plant Cell Tiss Organ Cult

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The medium used for the growth of anthocyanin-accumulating wild carrot (D. carota) suspension cultures contained ammonia as a sole nitrogen source and was buffered with succinate. Ammonia was the first nutrient to be completely utilized.The uptake of carbohydrate, phosphate and succinate continued after ammonia depletion. Biomass accumulation was faster and greater when sucrose was initially present in the medium than when glucose was present. When sucrose was provided in the medium it was rapidly hydrolysed to glucose and fructose and the fructose was used preferentially to glucose. Anthocyanin accumulation was rapid after ammonia fell below 3 mM and until the pH of the medium rose from 4.5 to 5.1 or 5.2.

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Section: Discussionsupporting

confidence: 67%

Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells

Dougall

Frazier

1989

Plant Cell Tiss Organ Cult

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“…The requirement of reduced nitrogen for continued embryo development (Wetherell and Dougall, 1976;Kamada and Harada, 1979) is also well established and was confirmed here. Carrot cultures have also been shown to grow on medium containing NH 4 + alone if the medium was buffered or continuously titrated (Dougall and Weyrauch, 1980). Furthermore, addition of NH 4 + and N0 3 -to culture media appears to provide a means whereby cultures continuously "titrate" the pH of their own medium.…”

Section: Discussionmentioning

confidence: 99%

SOMATIC EMBRYOGENESIS OF CARROT IN HORMONE‐FREE MEDIUM: EXTERNAL pH CONTROL OVER MORPHOGENESIS

Smith

Krikorian

1990

American J of Botany

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Cultures of preglobular stage proembryos (PGSPs) were initiated from mechanically wounded mature zygotic embryos of carrot, Daucus carota, on a hormone-free, semisolid medium. These PGSPs have been maintained and multiplied for extended periods without their progression into later embryo stages on the same hormone-free medium containing 1 mM NH4+ as the sole nitrogen source. Sustained maintenance of cultures comprised exclusively of PGSPs was dependent on medium pH throughout the culture period. Best growth and multiplication of PGSP cultures occurred when the pH of unbuffered, hormone-free medium fell from 4.5 to 4 over a 2-week period or when buffered medium was titrated to pH 4. If the hormone-free medium was buffered to sustain a pH at or above 4.5, PGSPs developed into later embryo stages. Maintenance with continuous multiplication of PGSPs occurred equally well on medium containing NH4+ or NH4+ and NO3-, but growth was poor with NO3- alone. Additional observations on the effects of medium components such as various nitrogen sources and levels, sucrose concentration, semisolid supports, type of buffer, borate concentration, activated charcoal, and initial pH that permit optimum maintenance of the PGSPs or foster their continued developmental progression into mature embryos and plantlets are reported. The influence of the pH of the hormone-free medium as a determinant in maintaining cultures as PGSPs or allowing their continued embryonic development are unequivocally demonstrated by gross morphology, scanning electron microscopy, and histological preparations.

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“…Preparation of four carrot cell extracts.-Two carrot cell lines, WC63.3b.f and WC38-1A, were produced by a subculturing procedure (7,8) from cells originally supplied by D. F. Wetherell, The University of Connecticut, Storrs, Ct. Each line was cultured in two different media, WC-1 A2688, an unimproved or stock culture medium (9) and WC-IMP A2687, an improved medium (8,10). An extract from each cell line in each medium was then prepared by the following procedure.…”

Section: Methodsmentioning

confidence: 99%

Anthocyanins Present in Cell Cultures of Daucus carota

Hemingson¹,

Collins²

1982

J. Nat. Prod.

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Abilities of organic acids to support growth and anthocyanin accumulation by suspension cultures of wild carrot cells using ammonium as the sole nitrogen source

Cited by 25 publications

References 14 publications

Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells

Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells

SOMATIC EMBRYOGENESIS OF CARROT IN HORMONE‐FREE MEDIUM: EXTERNAL pH CONTROL OVER MORPHOGENESIS

Anthocyanins Present in Cell Cultures of Daucus carota

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