Aberrant Expression of miR-203 and Its Clinical Significance in Gastric and Colorectal Cancers

Chiang, Yeunpo; Song, Yongxi; Wang, Zhenning; Chen, Yue; Yue, Zhenyu; Xu, Hui; Xing, Chengzhong; Liu, Zhuangkai

doi:10.1007/s11605-010-1367-8

Cited by 88 publications

(65 citation statements)

References 33 publications

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“…For example, miR-203 is able to inhibit the proliferation, migration and metastasis of triple-negative breast and lung cancer cells (20,21). The expression of miR-203 is associated with tumor size, advanced Borrmann type and pT category of the GC (22). In addition, miR-148b is able to suppress cell growth in GC and colorectal cancer by targeting cholecystokinin B receptor (8,23), indicating that a number of miRNAs have the same function in different types of cancer.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

et al. 2017

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Abstract. Numerous microRNAs (miRNAs/miRs) are involved in suppressing or promoting the formation of cancer. However, to the best of our knowledge, the role of miRNA-1258 in gastric cancer (GC) has not previously been investigated. Our previous study demonstrated an increased expression of heparanase (HPSE) in GC tissues and HPSE-facilitated invasion and metastasis of GC cells. Consequently, in the present study, the function of miR-1258 in the invasion and metastasis of GC cells was investigated to determine whether miR-1258 is associated with GC through HPSE. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to determine miR-1258 expression in GC cell lines and tissues. A Transwell cell invasion assay and an MTT proliferation assay were used to investigate the effects of miR-1258 on the invasion and proliferation of one of the cell lines, SGC-7901 cells, in vitro. The effect of miR-1258 on SGC-7901 cell metastasis was investigated using an in vivo tumor metastasis formation assay. Western blot analysis and RT-qPCR were used to investigate whether HPSE was a target of miR-1258 in GC. The expression of miR-1258 was significantly downregulated in 3 GC cell lines and 116 GC tissues compared with controls (all P<0.001). An association was identified between decreased miR-1258 expression level and increased age (P=0.042), advanced pathological tumor stage (P=0.027) and positive lymphatic vessel invasion (P=0.044) in patients with GC. Furthermore, the upregulation of miR-1258 expression suppressed SGC-7901 cell invasion in vitro (P<0.001) and inhibited SGC-7901 cell metastasis in vivo (P=0.016). The western blot analysis and RT-qPCR results indicated that miR-1258 downregulated the expression of HPSE at the translational level. The results of the present study indicate that miR-1258 acts as a tumor suppressor to inhibit invasion and metastasis by targeting HPSE. Therefore, miR-1258 may serve as a novel biomarker and therapeutic target in the treatment of GC.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

et al. 2017

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“…Regarding miR-203 expression in GC tissue, Chiang et al revealed that, although expression of miR-203 did not differ significantly between GC tissues and normal gastric mucosa, low expression of miR-203 was significantly correlated with large tumor size, macroscopic type, and advanced pathological T stage [39]. Moreover, Saad et al reported a tendency for lower miR-203 expression in GC tissue than normal gastric tissue, even though that study focused on a small patient cohort [40].…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNA-203 predicts metastases, early recurrence, and poor prognosis in human gastric cancer

et al. 2015

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Background Metastasis is a major cause of death in patients with gastric cancer (GC). MicroRNAs (miRNAs) relating to the epithelial-mesenchymal transition (EMT) control GC progression and metastasis. The aim of this study was to evaluate serum EMT-associated miRNAs for metastatic and prognostic noninvasive biomarkers in GC. Methods In the first step of this study (preliminary experiments), we selected candidate miRNAs associated with metastasis by analyzing the expression of the miR-200 family (miR-200a, miR-200b, miR-200c, miR-141, and miR-429) and miR-203 in serum samples from stage I (n = 12) and stage IV (n = 12) GC patients. The second phase involved the independent validation of candidate miRNAs in serum specimens from 130 patients with GC and 22 controls. Results Based on the preliminary experiments, miR-203 was selected as the candidate serum miRNA that was most closely associated with metastasis. Validation analysis revealed that serum miR-203 levels were significantly lower in stage IV than stage I-III GC patients. Serum miR-203 expression was significantly lower in GC patients with a higher T stage, vessel invasion, and lymph node, peritoneal, and distant metastases. Low expression of serum miR-203 was significantly associated with poor diseasefree and overall survival. Multivariate analysis revealed that low serum miR-203 expression was an independent predictive marker for lymph node, peritoneal, and distant metastases and a poor prognosis in patients with GC. Conclusions Serum miR-203 has the potential to serve as a noninvasive biomarker for prognosis and to predict metastasis in patients with GC.

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“…∆∆C t indicates the difference in the ∆C t value between cancer tissue and the corresponding control (∆∆C t = ∆C t cancer -∆C t control ) and ∆C t is the difference of the C t value between the target and U6 (∆C t = C t target -C t U6 ). Finally, the 2 -∆∆Ct value (fold value) was calculated and distinguished as 1-fold, and a fold value of less than 1-fold was defined as low expression (15,21). Differences in miRNA expression were measured by comparing the values of ΔC t cancer and ΔC t control, and statistical differences in miRNA expression levels were determined using a paired t-test in cancer tissues and cancer cell lines relative to non-tumor counterparts, as well as comparing the effect of miR-194 on cell proliferation in HCT-116 cells by MTT assay.…”

Section: Real-time Pcrmentioning

confidence: 99%

“…Our previous study showed similar results, in that miR-203 was significantly downregulated in CRC by real-time PCR assay. Also, the low expression of miR-203 was correlated with increased tumor size and advanced depth of invasion (15). Furthermore, Georges et al (16) reported that miR-192 and -215 were downregulated by microarray and qRT-PCR methods in human CRC cell lines, and these miRNAs, as tumor suppressors, led to cell cycle arrest.…”

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confidence: 98%

microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

Chiang

Song

Wang

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. microRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have aberrant expression in a number of tumor types. miR-192, -194 and -215 have not been comprehensively investigated using a large number of cases in colorectal cancer (CRC). We extracted total RNA from 107 CRC tissues and three CRC cell lines. Following polyadenylation and reverse transcription, the expression levels of miR-192, -194 and -215 were determined for evaluation of the association between expression levels and clinicopathological characteristics by a quantitative real-time polymerase chain reaction (real-time PCR) method. Finally, we studied the impact of miR-194 on cell proliferation in HCT-116 cells by MTT assay. miR-192, -194 and -215 were significantly downregulated in CRC tissues (all p<0.001, paired t-test) and cancer cell lines (all p<0.05) compared to non-tumor counterparts. Moreover, the expression levels of miR-192, -194 and -215 were demonstrated to be associated with increased tumor sizes (p=0.027, p=0.018, and p=0.027, respectively; Mann-Whitney U test). Also, there were marked correlations among these miRNAs in CRC tissues (all p<0.001, Pearson's regression analysis). Furthermore, we found that the overexpression of miR-194 could significantly inhibit cell proliferation in may be important biological markers as tumor suppressors in the carcinogenesis of CRC.

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Aberrant Expression of miR-203 and Its Clinical Significance in Gastric and Colorectal Cancers

Abstract: miR-203 may be related to the proliferation and invasion of gastric and colorectal cancers.

Cited by 88 publications

References 33 publications

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

MicroRNA-1258: An invasion and metastasis regulator that targets heparanase in gastric cancer

Circulating microRNA-203 predicts metastases, early recurrence, and poor prognosis in human gastric cancer

microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

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