A validated HPTLC method for determination oftrans-caryophyllene from polyherbal formulations

Abstract

Formulations of traditional medicines are usually made up of a complex mixture of herbs. However, effective quality control methods in order to select materials of the right quality are lacking. 'Amukkara choornam' is a polyherbal Siddha formulation used for gastritis, spleen enlargement, leucorrhoea, hiccups, anaemia, tuberculosis and kappa diseases. Trans-caryophyllene is an important constituent present in the ingredients of this formulation. In a literature survey, it was found that there is no such method for the quantification of trans-caryophyllene except gas chromatography or gas chromatography-mass spectroscopy (GC-MS). So, a high performance thin layer chromatography (HPTLC) method was developed and validated for the quantification of trans-caryophyllene in amukkara choornam. Pre-coated silica gel 60F-254 plates (10 × 10 cm²) were used for the analysis. The solvent system consisted of toluene-ethyl acetatate (9 : 3, v/v), and trans-caryophyllene was detected at 260 nm. The developed method was validated for linearity (R² = 0.9996 ± 0.0034), limit of detection (LOD) (0.101 ng), limit of quantification (LOQ) (0.639 ng), accuracy (% recovery = 97.19 ± 1.204), and precision (CV < 5%, for both intra-day and inter-day precisions). The levels of trans-caryophyllene were found to be 3.5-4.10 µg per gram of herbal products.

Simultaneous Determination of Menthol and Eucalyptol by the Densitometric HPTLC Method in Some External Analgesic Formulations

“…Menthol was estimated using paper chromatography after treated with p-aminobenzaldehyde to produce xanthate derivative with red color (12). A validated highperformance thin layer chromatography (HPTLC) method was developed to estimate eucalyptol in a polyherbal formulation known as 'Amukkara choornam' (13). Many marketed formulations contain menthol and euclyptol among other ingredients.…”

β-Caryophyllene, A Natural Dietary CB2 Receptor Selective Cannabinoid can be a Candidate to Target the Trinity of Infection, Immunity, and Inflammation in COVID-19

“…BCP improved systemic inflammation and oxidative status with no hepatotoxicity, as with nonsteroidal anti-inflammatory drugs (Ames-Sibin et al, 2018). It also reduced nausea, epigastric pain, and diarrhea, and improved gastrointestinal activity (Patra et al, 2010). BCP was also found to promote wound healing by modulating numerous signaling pathways (Parisotto-Peterle et al, 2020).…”

“…Many formulations containing BCP have been developed, including Amukkara Choornam (Patra et al, 2010), CIN-102, a coated pellets and matrix mini-tablet (Nieto-Bobadilla et al, 2015), and PipeNig ® -FL, a high standardized content of BCP (Geddo et al, 2019). BCP is highly lipophilic, less soluble in water and, upon exposure to air, it easily oxidizes.…”

Beta-Caryophyllene, a Cannabinoid Receptor Type 2 Selective Agonist, in Emotional and Cognitive Disorders

“…Several BCP-containing formulations have been developed, including Amukkara Choornam [ 133 ], CIN-102 [ 134 ], and PipeNig ® -FL [ 135 ]. In particular, a 2022 clinical trial involving 30 patients revealed that Amukkara Chooranam, a polyherbal formulation containing BCP, was able to significantly improve clinical assessment parameters in children with autism spectrum disorder [ 136 ].…”