Simultaneous Determination of Menthol and Eucalyptol by the Densitometric HPTLC Method in Some External Analgesic Formulations

Alam, Prawez; Alqasoumi, Saleh I.; Abdel‐Kader, Maged S.

doi:10.1093/chromsci/bmv075

Cited by 12 publications

(7 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LOQ of the proposed method was found to be 8.5 μg/spot. These values are relatively higher than those obtained in HPTLC studies where values are in the ng ranges (Abdel-Kader et al, 2016, Alam et al, 2016). The less sensitivity of the gel resulted from the high molecular weight of the enzymes comparing with the small molecules quantified on HPTLC.…”

Section: Resultscontrasting

confidence: 55%

Utilization of gel electrophoreses for the quantitative estimation of digestive enzyme papain

Muharram

Abdel‐Kader

2017

Saudi Pharmaceutical Journal

Self Cite

View full text Add to dashboard Cite

SDS-PAGE densitometric method for analysis of papain in pharmaceutical formulations was developed and validated for the first time. Standard and samples were mixed with SDS sample buffer and denatured at 95 °C for 5 min and the gel was run at 20 mA and 200 V for 30-40 min in SDS-PAGE buffer. Gels were stained in Coomassie blue solution and distained by 5% methanol and 10% acetic acid. Destained gels were imaged and analyzed using the ChemiDoc™ XRS+ System. Bands of papain appeared at value 0.78 ± 0.03 corresponding to molecular weight 23406 Da between proteins with molecular weight 31,000 and 21,500 Da of the broad range protein standard. The generated calibration curve was used for quantitative estimation of papain in pharmaceutical formulations. The developed method was validated for precision, accuracy, specificity and robustness as described by the ICH guidelines. The proposed method gives an alternative approach for enzymes and protein analysis.

show abstract

Section: Resultscontrasting

confidence: 55%

Utilization of gel electrophoreses for the quantitative estimation of digestive enzyme papain

Muharram

Abdel‐Kader

2017

Saudi Pharmaceutical Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…ROS played a dual role in oxidative stress: directly destroying cellular components (DNA, proteins and lipids) and activating cellular machinery, which damages skin connective tissues jointly, leading to photoaging (38). Usually, endogenous antioxidant enzymes, such as SOD, CAT and HO-1, work in synchrony to eliminate ROS (36,37). However, excessive ROS induced by repetitive UVB irradiation overwhelms the endogenous antioxidant enzymes, and the accumulation of MDA is a marker for evaluating tissue oxidation (12).…”

Section: Discussionmentioning

confidence: 99%

Photoprotective Effect of Artemisia sieversiana Ehrhart Essential Oil Against UVB‐induced Photoaging in Mice

Zhou

Zhang

et al. 2021

Photochem & Photobiology

View full text Add to dashboard Cite

Photoaging refers to the extrinsic aging resulting from ultraviolet (UV) irradiation, which impacts skin appearance and is accompanied by the risk of skin carcinoma. Developing natural products as photoprotective agents is of great interest in cosmetic industry nowadays. The present study aimed at investigating the possible use of Artemisia sieversiana Ehrhart essential oil (AEO) for the prevention of photoaging induced by UVB. AEO was characterized by chamazulene, which accounted for 38.92% among total 51 identified compounds. In in vitro assays, AEO was found to be a moderate antioxidant and good UVB filter with photostability. A UVB‐induced photoaging mice model was established with three AEO formulations (0.1%, 0.5% and 1.5%, w/w) topically applied prior to UVB irradiation. The activities of catalase, particularly superoxide dismutase of skin increased, while malondialdehyde content decreased in AEO groups as compared with model controls. The production of matrix metalloproteinases (MMP‐1 and MMP‐3) and depletion of hydroxyproline in skin were inhibited by AEO in a dose‐dependent manner. Histological evaluation indicated that AEO decreased epidermal thickness, inflammatory cell infiltration, collagen degradation and elastin aberrance. These findings indicated that AEO could be a promising sunscreen agent in protecting the skin against photoaging.

show abstract

“…For the simultaneous quanti ication of protodioscin and prototribestin, a simple and repeatable HPTLC method was developed (Alam et al, 2016) using WINCAT software. HPTLC analysis is a strong recognition method.…”

Section: Figure 5: Structure Of Cordiofolosidementioning

confidence: 99%

A Review of Markers in Ayurveda for Anti-Inflammatory Drugs

Vineetha

Shanmugasundaram

et al. 2021

ijrps

View full text Add to dashboard Cite

From the ancient days itself the ayurvedic medicine has special importance. This ayurvedic system of medicine originated in India. In India nearly 70% of people use ayurvedic system of medicine along the western system of medicine. The Ayurveda mainly come under three classes are vata, pitta and the kapha. Chemically identified constituents of an herbal medicine that are of concern for quality control purposes are known as markers. Some of the markers are widely used for anti-inflammatory agents are Boswellia, sunthi, gokshuru, guduchi, guggulu this marker possess anti-inflammatory action that reduces pain, swelling of joints, Arthritis, joint complaints, Muscular injuries etc. The chromatographical / spectroscopical analytical development can be done for markers. The analytical development possess the way to the analytical information about the markers. The technique such as HPLC, HPTLC, LC-MS, UPLC-PDA are properly validated by ICH guidelines using validation parameters such as accuracy, recovery, precision, LOD, LOQ, Robustness etc. For many markers there is lack of development of Hyphenated techniques. By increasing the analytical techniques, research is prone to be more effective and successful to promote quality, safety and efficacy.

show abstract

Simultaneous Determination of Menthol and Eucalyptol by the Densitometric HPTLC Method in Some External Analgesic Formulations

Cited by 12 publications

References 9 publications

Utilization of gel electrophoreses for the quantitative estimation of digestive enzyme papain

Utilization of gel electrophoreses for the quantitative estimation of digestive enzyme papain

Photoprotective Effect of Artemisia sieversiana Ehrhart Essential Oil Against UVB‐induced Photoaging in Mice

A Review of Markers in Ayurveda for Anti-Inflammatory Drugs

Contact Info

Product

Resources

About