A single, low dose oral antigen exposure in newborn piglets primes mucosal immunity if administered with CpG oligodeoxynucleotides and polyphosphazene adjuvants

Pasternak, J. Alex; Ng, Siew Hon; Wilson, Heather L.

doi:10.1016/j.vetimm.2014.08.006

Cited by 13 publications

(16 citation statements)

References 43 publications

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“…Our study is the first to demonstrate the use of PCEP and ABD as adjuvants in a poultry vaccine. Interestingly, like the above-mentioned findings in cattle, sheep, pigs, and mice (7,13,20,51), the addition of PCEP with inactivated IBHV in our experiments resulted in a significant additive effect on antibody response. Similarly, the combined effect of the PCEP and ABD2 (in Trial 3) vaccine formulation containing Vaccine against IBHV infection inactivated virus showed significantly higher antibody responses compared with hexon protein-based vaccine formulations.…”

Section: Discussionsupporting

confidence: 84%

Administration of Poly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) and Avian Beta Defensin as Adjuvants in Inactivated Inclusion Body Hepatitis Virus and its Hexon Protein-Based Experimental Vaccine Formulations in Chickens

Dar

Tipu²,

Townsend³

et al. 2015

Avian Diseases

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Inclusion body hepatitis (IBH) is one of the major infectious diseases adversely affecting the poultry industry of the United States and Canada. Currently, no effective and safe vaccine is available for the control of IBH virus (IBHV) infection in chickens. However, based on the excellent safety and immunogenic profiles of experimental veterinary vaccines developed with the use of new generation adjuvants, we hypothesized that characterization of vaccine formulations containing inactivated IBHV or its capsid protein hexon as antigens, along with poly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) and avian beta defensin 2 (ABD2) as vaccine adjuvants, will be helpful in development of an effective and safe vaccine formulation for IBH. Our data demonstrated that experimental administration of vaccine formulations containing inactivated IBHV and a mixture of PCEP with or without ABD2 as an adjuvant induced significantly higher antibody responses compared with other vaccine formulations, while hexon protein-based vaccine formulations showed relatively lower levels of antibody responses. Thus, a vaccine formulation containing inactivated IBHV with PCEP or a mixture of PCEP and ABD2 (with a reduced dosage of PCEP) as an adjuvant may serve as a potential vaccine candidate. However, in order to overcome the risks associated with whole virus inactivated vaccines, characterization of additional viral capsid proteins, including fiber protein and penton of IBHV along with hexon protein in combination with more new generation adjuvants, will be helpful in further improvements of vaccines against IBHV infection.

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Section: Discussionsupporting

confidence: 84%

Administration of Poly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) and Avian Beta Defensin as Adjuvants in Inactivated Inclusion Body Hepatitis Virus and its Hexon Protein-Based Experimental Vaccine Formulations in Chickens

Dar

Tipu²,

Townsend³

et al. 2015

Avian Diseases

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“…Después del destete, pueden ser detectadas respuestas inmunes a ingredientes del alimento, a raíz de la cual, el animal va adquiriendo tolerancia al alimento, aunque esto pueda tomar algún tiempo (Pasternak, Hon & Wilson, 2014).…”

Section: Discussionunclassified

Adición de Enterococcus faecium mejora poblaciones celulares inmunes y anticuerpos vacunales de lechones destetos

Franco

Galeano

Suescún

2016

Rev. Lasallista Investig.

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“…In previous reports, oral inoculation with vaccines was frequently ineffective due to instability in the digestive tract, weak antigen uptake from mucosal surfaces, and difficulty inducing immune responses against administered antigens (Moyle et al 2004). Oral administration of antigens in combination with adjuvant may enhance the induction of mucosal immune responses against combined antigens and therefore has the potential to control pathogens at their entry point (Moyle et al 2004;Fingerut et al 2006;Pasternak et al 2014). In particular, locally-produced intestinal IgA binds antigens to mask their epitopes, and subsequently prevents microbial colonization and penetration of the gut wall (Corthesy 2009).…”

Section: Discussionmentioning

confidence: 99%

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

Hur

Kim

et al. 2015

Veterinary Quarterly

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Results: All groups with single, double, and triple inoculations of the ghost showed higher humoral and mucosal immune responses than the control group. In particular, the groups with intramuscular double and triple inoculations showed significantly higher immune responses. In addition, oral inoculation with a combination of the ghost and MONTANIDE IMS 1113 (MI1113) resulted in high and prolonged induction of intestinal IgA levels. Conclusion: These results indicated that both systemic and mucosal immunity against ETEC fimbrial antigens expressed by the ghost are induced by intramuscular booster inoculation with the ghost, and that addition of M1113 to the ghost was found to result in prominent induction of mucosal immunity through oral inoculation.

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A single, low dose oral antigen exposure in newborn piglets primes mucosal immunity if administered with CpG oligodeoxynucleotides and polyphosphazene adjuvants

Cited by 13 publications

References 43 publications

Administration of Poly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) and Avian Beta Defensin as Adjuvants in Inactivated Inclusion Body Hepatitis Virus and its Hexon Protein-Based Experimental Vaccine Formulations in Chickens

Administration of Poly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) and Avian Beta Defensin as Adjuvants in Inactivated Inclusion Body Hepatitis Virus and its Hexon Protein-Based Experimental Vaccine Formulations in Chickens

Adición de Enterococcus faecium mejora poblaciones celulares inmunes y anticuerpos vacunales de lechones destetos

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

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