<i>Salmonella</i>ghosts expressing enterotoxigenic<i>Escherichia coli</i>k88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

Hur, Jin; Kim, Chan Song; Eo, Seong Kug; Park, Sang-Youel; Lee, John Hwa

doi:10.1080/01652176.2015.1029598

Cited by 9 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2013; Hur et al . 2015; Cao et al . 2018); (ii) the use of special media, toxic or expensive chemical inducers is avoided and contamination risks of culture handling are minimized as gene expression is triggered by a simple increase in temperature from 28°C or 30 to 42°C (Valdez‐Cruz et al .…”

Section: Discussionmentioning

confidence: 99%

“…The thermo-inducible expression system, based on the phage lambda promoter pR and/or pL regulated by the thermo-labile cI857 repressor, has been widely utilized in the production of BGs and recombinant proteins due to the following advantages: (i) the system works in a variety of bacteria (Hu et al 2013;Hur et al 2015;Cao et al 2018); (ii) the use of special media, toxic or expensive chemical inducers is avoided and contamination risks of culture handling are minimized as gene expression is triggered by a simple increase in temperature from 28°C or 30 to 42°C (Valdez-Cruz et al 2010); and (iii) after induction, pR and/or pL functions as a strong promoter.…”

Section: Discussionmentioning

confidence: 99%

“…A BG is an empty, nonliving Gram-negative bacteria-derived cell envelope with intact cellular morphology and structure (Montanaro et al 2015). As compared with traditional formalin-inactivated vaccines, BGs elicit stronger immune responses, thereby providing more effective protection against pathogen challenge (Hu et al 2013;Cao et al 2018;Hoseini Shahidi et al 2019), and can also be used as an adjuvant or versatile delivery vehicle for heterologous antigens, drugs and nucleic acids (Chen et al 2014;Ganeshpurkar et al 2014;Hur et al 2015;Xia et al 2016). Typically, BGs are produced by controlled expression of the lysis gene E of bacteriophage phiX174 in a temperature inducible expression system consisting of the lambda promoter pR or/and pL and the thermo-sensitive repressor cI857 (Valdez-Cruz et al 2010;Fu and Lu 2013).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

Gong

Gao

et al. 2020

J Appl Microbiol

View full text Add to dashboard Cite

Aims To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo‐inducible λcI857‐pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs). Methods and results The promoter pL mutant was identified by random mutagenesis and site‐directed mutagenesis. The results showed that a T → 35C mutation in the pL promoter was responsible for the phenotype alteration. Under the same induction conditions, the lysis rates of the modified lytic system on Escherichia coli and Salmonella enteritidis were significantly lower than that of the control, while the lysis rates of Escherichia coli with the thermo‐inducible lytic system were significantly higher than that of S. enteritidis with the corresponding plasmid (P < 0·05). Conclusions Increasing the heat stability of the thermo‐inducible lytic systems decreased lysis efficiency during the production of BGs. There exist differences in the lysis efficiency of thermo‐inducible lytic systems between different bacterial strains. Significance and Impact of the Study These findings enrich current knowledge about modifications to thermo‐inducible systems and provide a reference for the application of these modified systems for the production of BGs and controlled gene expression in bacteria.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

Gong

Gao

et al. 2020

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

Chemically induced bacterial ghosts: a novel approach for advancing biomedical applications

Park

2023

Mol. Cell. Toxicol.

View full text Add to dashboard Cite

Background Bacterial ghosts (BGs) are empty cell envelopes derived from bacteria, making them safe and non-replicative, and BGs have shown great potential as a vaccine platform. Specifically, chemically induced BGs are generated by selectively removing the cytoplasmic content of bacterial cells while preserving the structural integrity of the cell envelope. Objective Generally, BGs are genetically engineered, but this is limited to Gram-negative bacteria. However, the utilization of chemically induced BGs can be extended to Gram-positive bacteria, resulting in empty bacterial envelopes that hold potential as a platform for drug delivery. Results Chemically induced BGs offer several advantages, including improved safety profile and immunogenicity, and efficient antigen presentation. Preclinical studies have yielded promising results, exhibiting enhanced immune responses and protection against diverse pathogens. Conclusion Chemically induced BGs represent a novel and promising approach for vaccine development, holding the potential for advancing disease prevention and public health. Purpose of review In this review, we discuss key aspects of chemically induced BGs, including their production principles, mechanisms of formation, characterization techniques, immunogenicity, and medical applications. We also discuss the challenges and direction of future research for optimizing production methods for chemically induced BGs, evaluating long-term safety, and undertaking clinical trials to assess their efficacy.

show abstract

A novel approach for construction of an inactivated typhoid vaccine candidate that effectively augments both humoral and cellular immune responses

Won

Kim

Lee

2017

Vaccine

View full text Add to dashboard Cite

Salmonellaghosts expressing enterotoxigenicEscherichia colik88ab, k88ac, k99, and fasa fimbrial antigens induce robust immune responses in a mouse model

Cited by 9 publications

References 41 publications

Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

Chemically induced bacterial ghosts: a novel approach for advancing biomedical applications

A novel approach for construction of an inactivated typhoid vaccine candidate that effectively augments both humoral and cellular immune responses

Contact Info

Product

Resources

About