A single dose of platelet-rich plasma improves the organization and strength of a surgically repaired rotator cuff tendon in rats

Dolkart, Oleg; Chechik, Ofir; Zarfati, Y.; Brosh, Tamar; Alhajajra, Fadi; Maman, Eran

doi:10.1007/s00402-014-2026-4

Cited by 54 publications

(34 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although animal models are suitable to observe the systemic effects of PRP, animal tissue physiology and sensitivity differ from those of human tissue. [25][26][27] Concerning the small amount of studies demonstrating the in vitro effects of different leukocyte concentrations of PRP, it was observed that these studies are on synoviocytes. [28] or other tissues such as human ligament fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

Şirin

Yılmaz

Isyar

et al. 2017

Eklem Hastalik Cerrahisi

View full text Add to dashboard Cite

ÖZAmaç: Bu çalışmada trombositten zengin plazma (TZP) içeriğindeki lökosit konsantrasyonunun ve TZP'nin ilaç taşıyıcı sistem kullanılarak uygulanmasının in vitro koşullarda kondrosit proliferasyonu üzerine olası etkileri araştırıldı. Hastalar ve yöntemler: Medikal veya konservatif tedaviye yanıt vermemiş ve total diz artroplastisi geçirmiş dokuz erkek hastanın (ort. yaş 65 yıl; dağılım 49-81 yıl) kanı iki formülasyon hazırlamak üzere kullanıldı: Düşük konsantrasyon lökositli TZP (2000-4000 lökosit/µL) saf TZP (S-TZP) olarak, yüksek konsantrasyon lökositli TZP (9000-11000 lökosit/µL) ise lökositten zengin TZP (L-TZP) olarak belirlendi. Numuneler kontrol grubu (grup 1), direkt S-TZP uygulanan kondrosit kültürleri (grup 2), direkt L-TZP uygulanan kondrosit kültürleri (grup 3), S-TZP uygulanan hidrojel ile kültürlenen kondrositler (grup 4) ve L-TZP uygulanan hidrojel ile kültürlenen kondrositler (grup 5) olmak üzere beş gruba ayrıldı. Tüm gruplarda hücre morfolojisi, canlılık ve proliferasyon bir prekondrosit belirteci olan evreye özgü embriyonik antijen 1'in (SSEA-1) ifadesi ile karşılaştırıldı. Bulgular: Maksimum hücre proliferasyonu ve SSEA-1 ifadesi grup 4'te gerçekleşti; SSEA-1 ifadesi ve hücre proliferasyonu arasında istatistiksel olarak anlamlı ilişki vardı. Sonuç: Çalışmamız TZP'nin lökosit konsantrasyonunun ve hidrojel gibi taşıyıcı sistemlerin önemini ve taşıyıcı sistem ile uygulanan L-TZP'nin kıkırdak hasarının tedavisinde TZP'nin konvansiyonel uygulamalarından daha etkin olduğunu gösterdi.Anahtar sözcükler: Eşkültür teknikleri; hidrojel; trombositten zengin plazma; evreye özgü embriyonik antijenler. ABSTRACTObjectives: This study aims to investigate the possible effects of leukocyte concentration in the content of platelet-rich plasma (PRP) and the administration of PRP using a drug delivery system on chondrocyte proliferation in vitro conditions. Patients and methods: Blood from nine male patients (mean age 65 years; range 49 to 81 years) with advanced stage osteoarthritis who had not responded to medical or conservative treatments and underwent total knee arthroplasty was used to prepare two formulations: PRP with low concentration leukocytes (2000-4000 leukocytes/µL) was designated as pure PRP (P-PRP), whereas PRP with high concentration leukocytes (9000-11000 leukocytes/µL) as leukocyte-rich PRP (L-PRP). Samples were divided into five groups as control group (group 1), chondrocyte cultures with P-PRP applied directly (group 2), chondrocyte cultures with L-PRP applied directly (group 3), chondrocytes co-cultured with P-PRP applied hydrogel (group 4), and chondrocytes co-cultured with L-PRP applied hydrogel (group 5). In all groups; cell morphology, viability and proliferation were compared with the expression of stagespecific embryonic antigen-1 (SSEA-1), a precondrocyte marker. Results: Maximum cell proliferation and SSEA-1 expression occurred in group 4, with a statistically significant correlation between SSEA-1 expression and cell proliferation. Conclusion: Our study showed the importance of leuko...

show abstract

Section: Discussionmentioning

confidence: 99%

Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

Şirin

Yılmaz

Isyar

et al. 2017

Eklem Hastalik Cerrahisi

View full text Add to dashboard Cite

show abstract

“…Biomechanical analysis showed that PRP administration resulted in a signifi cantly higher maximal load and stiffness as compared to nontreated animals at week 3. Histological assessment showed that collagen birefringence was signifi cantly higher in PRP shoulders, indicating improved organization [ 12 ]. Ersen et al designed an experimental study for rotator cuff repair including four group (1) no repair, (2) primary repair, (3) repair plus PRP injections into the tendon-bone interface, and (4) repair plus PRP absorbed from a sponge carrier to the tendonbone interface.…”

Section: Prp In Tendon-bone Healingmentioning

confidence: 99%

Updated Animal Models in Orthopaedic Research

Öztuna

2015

European Instructional Lectures

View full text Add to dashboard Cite

Cionne et al. designed a study to characterize the early infl ammatory phase of fl exor tendon healing with the goal of identifying infl ammationrelated targets for future treatments. Canine fl exor tendons were transected and repaired with core and running epitenon suture. A controlled passive motion rehabilitation protocol was started on the fi rst post-operative day. The infl ammatory response was monitored for 9 days. Temporal changes in immune cell populations and gene expression of infl ammation-, matrix degradation-, and extracellular matrixrelated factors were examined. The most dramatic effect was a greater than 4,000-fold up-regulation in the expression of the proinfl ammatory factor IL-1β. While an infl ammatory response is likely necessary for healing to occur, high levels of pro-infl ammatory cytokines may result in impaired tendon healing. The tendon treatment approaches should include the modulation strategies of the infl ammatory phase of healing [ 37 ]. AbstractThere has been growing innovation in Orthopaedic basic science research over the past years. This study presents the newest knowledge about the musculoskeletal tissue healing which were investigated by animal studies and recently published in the major Orthopaedic journals. These studies demonstrate some modifi cations in methodology of animal models and a multidisciplinary approach to understanding the mechanisms underlying tissue healing and regeneration. Besides, the effects of gene therapies, different molecules, growth factors, some drugs which are widely used in daily practice and popular modalities (like ESWT and PRP) on tissue regeneration are summarized in this manuscript.

show abstract

“…Because of its autogenous origin, relative ease of preparation, and excellent safety profile PRP was introduced as a promising therapeutic agent in sports medicine. In vivo studies with animal models showed that PRP administration decreased tendon repair time while increasing collagen-fiber organization, vascularity and tensile strength [9,10]. In vitro studies reported that PRP treatment increased cell proliferation and growth factor expression [9], providing the scientific basis for further E. Amar and N. Snir contributed equally to this work.…”

Section: Introductionmentioning

confidence: 99%

Platelet-rich plasma did not improve early healing of medial collateral ligament in rats

Amar

Snir

Sher

et al. 2015

Arch Orthop Trauma Surg

Self Cite

View full text Add to dashboard Cite

show abstract

A single dose of platelet-rich plasma improves the organization and strength of a surgically repaired rotator cuff tendon in rats

Cited by 54 publications

References 19 publications

Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

Updated Animal Models in Orthopaedic Research

Platelet-rich plasma did not improve early healing of medial collateral ligament in rats

Contact Info

Product

Resources

About