Multiple myeloma (MM), a plasma cell (PC) malignancy, is the second most common blood cancer. Despite extensive research, disease heterogeneity is poorly characterized, hampering efforts for early diagnosis and improved treatments. Here, we apply scRNA-seq to study the heterogeneity of 40 individuals along MM progression spectrum including 11 healthy controls, demonstrating high interpatient variability that can be explained by expression of known MM drivers and additional putative factors. We identify extensive sub-clonal structures for 10/29 patients. In asymptomatic patients with early disease and in minimal residual disease post-treatment, we detect rare tumor-PC with similar molecular characteristics of active myeloma, with possible implications for personalized therapies. Single cell analysis of rare circulating-tumor-cells (CTC) allows for accurate liquid biopsy and detection of malignant PC, which reflect the patient BM disease. Our work establishes scRNA-seq for dissecting blood malignancies and devising detailed molecular characterization of tumor cells in symptomatic and asymptomatic patients.

Late reconstruction for chronic ruptures of the distal biceps using allograft tissue is a safe and effective solution for symptomatic patients with functional demands in forearm supination and elbow flexion. While there are several graft options, the literature supports good results with Achilles tendon allografts. Further studies are needed to evaluate the clinical outcomes of other allograft options.

The purpose of this study was to describe the surgical technique, and evaluate and analyze the clinical outcomes of patients who underwent tibial tubercle realignment in our institution. Reported results of tibial tubercle transfer for patellofemoral malalignment vary considerably. Most authors rely mainly on the passive intraoperative patellar tracking test in determining the new location of the tibial tubercle. Conversely, the senior author relocates the tibial tubercle based on the intraoperative active and passive patellar tracking tests and the condition of the patellar articular surface. The medical records of 66 consecutive patients who underwent 80 tibial tubercle relocation procedures due to patellar instability or patellofemoral pain and maltracking were reviewed. Fifty-five patients with 69 operated knees participated in designated follow-ups which were conducted according to a predesigned protocol. The median follow-up was 6.2 years (range 2-13 years). Clinical outcomes significantly improved compared to the preoperative state; 72.5% of operated knees demonstrated good-to-excellent results according to the Lysholm and Karlsson scores, and physical findings supported the positive effect of the procedure. A statistically significant positive correlation was found between clinical outcomes and male gender, the absence of moderate to severe patellar chondral damage, and instability as the dominant preoperative symptom. Postoperative complication rate was low and patients with longer postoperative follow-ups demonstrated better clinical outcomes. The presented method of tibial tubercle transfer provided a good long-lasting surgical solution for patients with patellar instability and patients with patellar maltracking and anterior knee pain. Good prognostic factors included male gender, clinical patellar instability as the dominant preoperative symptom and intact patellar articular cartilage.

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