Platelet-rich plasma did not improve early healing of medial collateral ligament in rats

Amar, Eyal; Snir, Nimrod; Sher, Osnat; Brosh, Tamar; Khashan, Morsi; Salai, Moshe; Dolkart, Oleg

doi:10.1007/s00402-015-2306-7

Cited by 13 publications

(9 citation statements)

References 31 publications

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“…The 31 in vivo ligament studies used various ligament models, including rat MCL, 2 canine ACL, 5,13,48,49,54,58,62,63 porcine ACL, 3,22,23,27,29,39,45 –47 rabbit MCL, 14,26,31,40,66 rabbit ACL/CCL, 1,32,52,59,67 and human ACL (Appendix Table A3). 50,55,60 Among in vivo studies, detection and performance biases were consistently high, whereas selection, attrition, reporting, and other biases were consistently low (Table 3).…”

Section: Resultsmentioning

confidence: 99%

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The Efficacy of Platelet-Rich Plasma for Ligament Injuries: A Systematic Review of Basic Science Literature With Protocol Quality Assessment

Kunze

Pakanati

Vadhera

et al. 2022

Orthopaedic Journal of Sports Medicine

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Background: Despite the existence of many clinical studies on platelet-rich plasma (PRP) interventions for ligamentous pathology, basic science consensus regarding the indications, mechanisms, and optimal composition of PRP for treating ligament injuries is lacking. Purpose: To (1) compare the efficacy of PRP in animal models of ligament injury with placebo and (2) describe the potential variability in PRP preparation using accepted classification systems. Study Design: Systematic review. Methods: The Cochrane Database of Systematic Reviews, Cochrane Central Register of Controlled Trials, PubMed, Embase, and Ovid MEDLINE were queried in April 2020 for in vivo and in vitro basic science studies regarding PRP use for ligament injury. Study design, results, PRP composition, and analyzed cellular and molecular markers were extracted, and outcomes relative to control models were documented. Bias was assessed using the SYRCLE risk-of-bias tool. Results: Included were 43 articles (31 in vivo and 12 in vitro studies) investigating the anterior cruciate ligament/cranial cruciate ligament (n = 32), medial collateral ligament (n = 6), suspensory ligament (n = 3), patellar ligament (n = 1), and Hock ligament (n = 1). Platelet concentration was reported in 34 studies (77.3%); leukocyte composition, in 12 (27.3%); and red blood cell counts, in 7 (15.9%). With PRP treatment, 5 of 12 in vitro studies demonstrated significant increases in cell viability, 6 of 12 in gene expression, 14 of 32 in vivo studies reported superior ligament repair via histological evaluation, and 13 in vivo studies reported superior mechanical properties. Variability in PRP preparation methods was observed across all articles, and only 1 study reported all necessary information to be classified by the 4 schemes we used to evaluate reporting. Among the in vivo studies, detection and performance bias were consistently high, whereas selection, attrition, reporting, and other biases were consistently low. Conclusion: Conflicting data on the cellular and molecular effects of PRP for ligament injuries were observed secondary to the finding that included studies were heterogeneous, limiting interpretation across studies and the ability to draw meaningful conclusions. Clinical trials and any causal relationship between PRP use in ligament injuries and its potential for regeneration and healing should be pursued with caution if based solely on basic science data.

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Section: Resultsmentioning

confidence: 99%

“… a References 1, 2, 3, 10, 11, 13, 14, 22, 23, 26, 27, 29, 30, 31, 36, 39, 41, 50, 51, 52, 56, 57, 63, 64, 65, 66. …”

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confidence: 99%

The Efficacy of Platelet-Rich Plasma for Ligament Injuries: A Systematic Review of Basic Science Literature With Protocol Quality Assessment

Kunze

Pakanati

Vadhera

et al. 2022

Orthopaedic Journal of Sports Medicine

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“…Many recent papers have reported that PRP is of benefit in the treatment of chronic tendon injuries, and many experimental studies have evaluated the effect of PRP on healing of MCL injury in animal models. [9][10][11] Yoshioka et al 9 evaluated the effect of PRP on MCL healing in a rabbit model and found that PRP treatment promoted healing of the MCL. In a rat model of MCL injury, Amar et al 10 found no evidence to support a benefit of PRP for MCL healing in the acute stage.…”

Section: Discussionmentioning

confidence: 99%

“…[9][10][11] Yoshioka et al 9 evaluated the effect of PRP on MCL healing in a rabbit model and found that PRP treatment promoted healing of the MCL. In a rat model of MCL injury, Amar et al 10 found no evidence to support a benefit of PRP for MCL healing in the acute stage. LaPrade et al 11 found that high doses of PRP decreased the quality of repair tissue in a rabbit model.…”

Section: Discussionmentioning

confidence: 99%

Autologous platelet-rich plasma therapy for refractory pain after low-grade medial collateral ligament injury

et al. 2020

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This study was performed to explore the effect of intra-articular injection of autologous platelet-rich plasma (PRP) on refractory pain after low-grade medial collateral ligament (MCL) injury. Methods: In total, 52 patients with refractory pain after low-grade knee MCL injury were treated by intra-articular injection of autologous PRP. Every patient received a 5-mL intraarticular injection of autologous PRP once weekly for 3 weeks. All patients were evaluated by the International Knee Documentation Committee (IKDC) Subjective Knee Evaluation Form score before treatment and 1, 3, and 6 months after treatment. Magnetic resonance imaging of the knee was performed to observe the healing of the MCL injury 6 months after treatment. Results: After one injection, the patients' pain was greatly decreased and they stopped taking nonsteroidal anti-inflammatory drugs. The IKDC Subjective Knee Evaluation Form scores were significantly better after than before treatment; however, there were no significant differences among the various post-treatment time points. Magnetic resonance imaging showed that the lowgrade MCL injury had completely healed, and no edema was present around the MCL. Conclusions: Intra-articular injection of autologous PRP is an effective treatment for refractory pain after low-grade MCL injury.

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“…A traumatic injury is followed by the formation of a platelet-rich hematoma, which releases growth factors and cytokines, which in turn stimulate the healing process 25 . Kajikawa et al 26 .applied PRP to rat patellar tendon and found an increase in the number of blood cells using labeled proteins.…”

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confidence: 99%

Biomechanical study of the effect of platelet rich plasma on the treatment of medial collateral ligament lesion in rabbits

Costa

Teixeira²,

Pádua³

et al. 2017

Acta Cir. Bras.

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Purpose: To evaluate the use of platelet-rich plasma in the early stages of healing of traumatic injury of the medial collateral ligament in the knee of rabbits. Methods: Thirty rabbits were subjected to surgical lesion of the medial collateral ligament. Of these, 16 were treated with platelet-rich plasma and 14 with saline (control). After 3 and 6 weeks of treatment, 50% of the animals from each group were sacrificed, and biomechanical tests were performed on the injured ligament to compare the tensile strength between the two groups. Results: Platelet-rich plasma significantly increased the tensile strength of the ligament in the groups treated after3 and 6 weeks. In the group treated with platelet-rich plasma vs. saline, the tensile strength values were 3192.5 ± 189.7 g/f vs. 2851.1 ± 193.1 g/f at3 weeks (p = 0.005) and 5915.6 ± 832.0 g/f vs. 4187.6 ± 512.9 g/f at 6 weeks (p = 0.0001). Conclusion:The use of platelet-rich plasma at the injury site accelerated ligament healing in an animal model, demonstrated by an increase in the tensile strength of the medial collateral ligament.

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Platelet-rich plasma did not improve early healing of medial collateral ligament in rats

Abstract: The addition of PRP to a healing MCL did not improve any of the outcome measures in this model.

Cited by 13 publications

References 31 publications

The Efficacy of Platelet-Rich Plasma for Ligament Injuries: A Systematic Review of Basic Science Literature With Protocol Quality Assessment

The Efficacy of Platelet-Rich Plasma for Ligament Injuries: A Systematic Review of Basic Science Literature With Protocol Quality Assessment

Autologous platelet-rich plasma therapy for refractory pain after low-grade medial collateral ligament injury

Biomechanical study of the effect of platelet rich plasma on the treatment of medial collateral ligament lesion in rabbits

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