Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

Şirin, Duygu Yaşar; Yılmaz, İbrahim; Isyar, Mehmet; Öznam, Kadir; Mahiroğulları, Mahir

doi:10.5606/ehc.2017.55186

Cited by 9 publications

(5 citation statements)

References 25 publications

(29 reference statements)

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“…As SSEA-1 is a marker for chondrocytic differentiation (13), no further morphological experiments, including flow cytometry or Giemsa staining were performed. Previous studies have also demonstrated that ESEM analysis reveals morphological abberations (10,13,37). …”

Section: Discussionmentioning

confidence: 87%

Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

Karaarslan

Batmaz²,

Yılmaz

et al. 2018

Exp Ther Med

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Non-steroidal anti-inflammatory drugs (NSAIDs) that are applied through oral, injectable or topical routes have been widely used in painful and inflammatory musculoskeletal diseases. The current study aimed to determine whether naproxen, an aryl acetic acid derivative with analgesic and anti-inflammatory effects, has a toxic effect on human chondrocytes. Samples containing monolayer primary chondrocyte cultures were prepared following resection from osteochondral tissues obtained from patients with gonarthrosis. Cell viability, toxicity and proliferation and levels of stage-specific embryonic antigen-1, a precursor to human prechondrocytes, were evaluated spectrophotometrically. The results from the untreated control group were compared with those of the study groups, where naproxen was administered in varying doses (1–1,000 µM). Surface morphologies of the cells were compared using inverted light and environmental scanning electron microscopy. Treatment groups were compared by analysis of variance with Tukey's honest difference post hoc test. P<0.01 was considered to indicate a statistically significant difference. The research revealed significant changes to proliferation and differentiation of chondrocytes in all treatment groups (P<0.01). Naproxen was demonstrated to suppress chondrocyte proliferation and differentiation, which may be an important factor to consider when prescribing this medication to patients.

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Section: Discussionmentioning

confidence: 87%

Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

Karaarslan

Batmaz²,

Yılmaz

et al. 2018

Exp Ther Med

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“…We believe that during centrifugation the denser granulocytes tend to stay trapped in the red cell layer of the centrifugate and thus are not captured in the PRP. This is significant for PRP prepared by single-spin methods because it shows that at least part of the controversy over whether leukocyte-rich (LRPRP) or leukocyte-poor (LPPRP) PRP [9][10][11] is clinically better for therapeutic purposes may be due to differences in leukocyte types rather than just leukocyte quantities. Few head-to-head trials have been performed comparing LRPRP and LPPRP, and no definitive conclusions can be drawn at this point.…”

Section: Discussionmentioning

confidence: 99%

Platelet-Rich Plasma Centrifugation Changes Leukocyte Ratios

Harrison

Bowler

Levins³

et al. 2021

Cureus

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Platelet-rich plasma (PRP) is usually described with respect to its platelet concentration and sometimes the concentration of erythrocytes and leukocytes. In this study, we examined the numbers of leukocyte subtypes in PRPs prepared by five different methods. Single spin PRP methods evaluated resulted in a significant increase in the percentage of lymphocytes and proportional/inverse reduction of the percentage of granulocytes in comparison to those percentages found in whole blood. We propose that the centrifugation process traps the denser granulocytes in the RBC layer more readily than lymphocytes and that this will vary by g force and time. The PRP preparation method may be clinically relevant, because the distribution of leukocytes may affect clinical outcomes.

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“…Most cytotoxicity studies used experimental setups involving tissues of living mammalian subjects (16,17). However, the results obtained with animal tissues may be different from those obtained with human tissue due to differences in sensitivity (18). In numerous studies where animal tissues were not used, commercial cell lines were used (19,20).…”

Section: Discussionmentioning

confidence: 99%

Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue

et al. 2019

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The present study aimed to evaluate the effects of dipyrone, an indispensable analgesic, anti-pyretic and anti-spasmodic used in emergency departments, on nucleus pulposus and annulus fibrosus cells in vitro . After surgical biopsy, primary cell cultures were prepared from intact intervertebral disc tissues. Dipyrone was administered to the cultures in the experimental groups except for the control group. The data obtained were statistically evaluated. The proliferation was identified to be suppressed via MTT analysis. The gene expression profile of the intervertebral disc cells in the dipyrone-treated groups was significantly changed. The expression of chondroadherin, cartilage oligo matrix protein, interleukin-1β and metalloproteinase (MMP)-19 genes were decreased, but MMP-13 and MMP-7 genes expressions were increased, as determined via reverse transcription-quantitative PCR. AO/PI staining revealed that no apoptotic or other type of cell death was detectable after administration of dipyrone does not mean that the drug is innocuous. The occurrence of cellular senescence and/or the halt of cell proliferation may also be important mechanisms underlying the adverse inhibitory effects of dipyrone. Therefore, prior to administering dipyrone in clinical practice, all possible adverse effects of this drug should be considered.

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Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

Cited by 9 publications

References 25 publications

Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

Platelet-Rich Plasma Centrifugation Changes Leukocyte Ratios

Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue

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