A simple and sensitive competitive bio-barcode immunoassay for triazophos based on multi-modified gold nanoparticles and fluorescent signal amplification

Bio-barcode detection technology and its research applications: A review

“…By optimizing the experimental conditions, a rather low linearity was achieved within a range from 4 pg/L to 40 ng/L, and the LOD was 1.06 pg/L. Zhang et al [98] detected the small molecule triazophos in water, rice, cucumber, cabbage and apple samples based on a competitive immunoassay with BCA. The method showed a linear range of 0.01–20 μg/L, and the LOD was 6 ng/L.…”

Optical Bio-barcode Nanobiosensors

“…The hapten-McAb-Au NPs probe was formed at the bottom of the microplate, the AuNPs labeled with small molecule can be removed by washing. After washing the free AuNPs, DTT has been used to release of 6-FAM-SH-ss DNA from the surface of labeled AuNPs on microplate and hence fluorescence signal can be detected (Zhang C. et al, 2017). The steps need to realized are given as follows: (Zhang C. et al, 2017).…”

“…1. (A) preparation of modified AuNPs with McAb and barcode DNA, (B) formed of hapten-McAb-AuNP probe complex and detection fluorescence signal(Zhang C. et al, 2017).…”

Affinity Sensing Strategies for the Detection of Pesticides in Food

“…Fluorescence is an emission phenomenon in which a fluorophore absorbs light (or electromagnetic radiation) and emits light into the visible spectrum with different (compared with the excitation) wavelength [ 80 ]. Zhang et al [ 78 ] designed a simple and highly sensitive immunoassay based on a competitive binding and bio-barcode amplification for the detection of triazophos. Gold nanoparticles were functionalized with antibodies and 6-carboxyfluorescein labeled single-stranded thiol-oligonucleotides (6-FAM-SH-ssDNAs); the fluorescence of 6-FAM was quenched by AuNPs.…”