A self-assembling RNA aptamer-based graphene oxide sensor for the turn-on detection of theophylline in serum

A fluorescence sensing platform of theophylline based on the interaction of RNA aptamer with graphene oxide

“…For example, Ling et al used FAM labeled RNA aptamer to prepare selectively turnon uorescent aptasensor for theophylline detection in serum. 12 However, due to the organic dye showed poor stability and less resistance to photobleaching, short uorescence lifetime, and long assay time, their further application was limited.…”

EpCAM aptamer-functionalized polydopamine-coated mesoporous silica nanoparticles loaded with DM1 for targeted therapy in colorectal cancer

“…16 APts have distinct advantages over traditional antibodies, including smaller size, lower immunogenicity, higher ratio of target accumulation, easier production, and higher in vivo stability. 17 Given their properties, APts have attracted much attention as molecular probes for cancer cell detection and targeted cancer therapy. [18][19][20][21] Epithelial cell adhesion molecule (EpCAM) APt is one of the DNA APts can specifically bind to EpCAM with high affinity.…”

Nanomaterial based aptasensors for clinical and environmental diagnostic applications

“…While the work of Zhu & co-workers applied a more conventional approach of using graphene oxide (GO) as a quencher in a green emissive uorophore modied aptamer, 73 the work of Xu et al & Ling et al revolved around a more novel "turning-on" signal, in an intricate play between the aptamer and its complementary uorophore-labelled helper DNA. 74,75 While their interaction in control samples keeps the helper DNA from interacting with nanosheets, the introduction of target molecules displaces the helper DNA from aptamers leading to their absorption on the surface of nanosheets causing it to quench (Fig. 4A).…”