A fluorescence sensing platform of theophylline based on the interaction of RNA aptamer with graphene oxide

Wu, Jianfeng; Gao, Xiang; Ge, Ling; Zhao, Guang‐Chao; Wang, Guangfeng

doi:10.1039/c9ra02475a

Cited by 16 publications

(13 citation statements)

References 29 publications

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“…Aptamers are short, single-stranded oligonucleotides or peptides that can act as important molecular tools for diagnostics and therapeutics. They are analogous to antibodies but offer many advantages over them as therapeutic reagents, including low cost, no immunogenicity, greater stability, easy synthesis and modification, and smaller molecular weight ( Song et al, 2014 ; Wu et al, 2019 ). An example is an aptasensor based on porous rGO/MoS 2 electrode covalently functionalized with an RNA aptamer targeted against human papillomavirus protein (HPV-16 L1 protein) ( Chekin et al, 2018 ).…”

Section: Graphene-based Materials In Virus Sensingmentioning

confidence: 99%

Human virus detection with graphene-based materials

Vermisoglou

Panáček

Jayaramulu

et al. 2020

Biosensors and Bioelectronics

153

117

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Our recent experience of the COVID-19 pandemic has highlighted the importance of easy-to-use, quick, cheap, sensitive and selective detection of virus pathogens for the efficient monitoring and treatment of virus diseases. Early detection of viruses provides essential information about possible efficient and targeted treatments, prolongs the therapeutic window and hence reduces morbidity. Graphene is a lightweight, chemically stable and conductive material that can be successfully utilized for the detection of various virus strains. The sensitivity and selectivity of graphene can be enhanced by its functionalization or combination with other materials. Introducing suitable functional groups and/or counterparts in the hybrid structure enables tuning of the optical and electrical properties, which is particularly attractive for rapid and easy-to-use virus detection. In this review, we cover all the different types of graphene-based sensors available for virus detection, including, e.g., photoluminescence and colorimetric sensors, and surface plasmon resonance biosensors. Various strategies of electrochemical detection of viruses based on, e.g., DNA hybridization or antigen-antibody interactions, are also discussed. We summarize the current state-of-the-art applications of graphene-based systems for sensing a variety of viruses, e.g., SARS-CoV-2, influenza, dengue fever, hepatitis C virus, HIV, rotavirus and Zika virus. General principles, mechanisms of action, advantages and drawbacks are presented to provide useful information for the further development and construction of advanced virus biosensors. We highlight that the unique and tunable physicochemical properties of graphene-based nanomaterials make them ideal candidates for engineering and miniaturization of biosensors.

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Section: Graphene-based Materials In Virus Sensingmentioning

confidence: 99%

Human virus detection with graphene-based materials

Vermisoglou

Panáček

Jayaramulu

et al. 2020

Biosensors and Bioelectronics

153

117

View full text Add to dashboard Cite

show abstract

“…Aptamers, which are single-stranded oligonucleotides or peptides, can be used for detection of whole viral particles. They are advantageous over antibodies because they are inexpensive and easily synthesized and modified [ 187 ]. RNA aptamers are degraded by nuclease activity of RNases, which makes it difficult to develop antiviral aptamers.…”

Section: Recent Applications Of Paper-based Analytical Devices For Virus Detectionmentioning

confidence: 99%

Paper-based analytical devices for virus detection: Recent strategies for current and future pandemics

Özer

Henry

2021

TrAC Trends in Analytical Chemistry

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The importance of user-friendly, inexpensive, sensitive, and selective detection of viruses has been highlighted again due to the recent Coronavirus disease 2019 (COVID-19) pandemic. Among the analytical tools, paper-based devices (PADs) have become a leading alternative for point-of-care (POC) testing. In this review, we discuss the recent development strategies and applications in nucleic acid-based, antibody/antigen-based and other affinity-based PADs using optical and electrochemical detection methods for sensing viruses. In addition, advantages and drawbacks of presented PADs are identified. Current state and insights towards future perspectives are presented regarding developing POC diagnosis platform for COVID-19. This review considers state-of-the-art technologies for further development and improvement in PADs performance for virus detection.

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“…One of the fragments was bound to a fluorophore, so that, in the absence of target molecule, its fluorescence was quenched by the proximity to GO, while in the presence of theophylline the formation of the corresponding complex allowed to have a fluorescence signal, with a very good sensitivity (1.6 × 10 −7 mol L −1 , Table 5, entry 4). A similar device was fabricated by Zhao and coworkers [24] using an ssRNA aptamer for theophylline, with excellent performances, also in the presence of an interferent with a very similar molecular structure like caffeine (Table 5, entry 5). Following the same principles, Cui and coworkers [71] were able to obtain a biosensor in which the signal was greatly amplified due to the presence of cryonase, an enzyme able to more quickly digest the aptamer-theophylline complex and thus to free the fluorescent probe (giving rise to a noteworthy signal), allowing a lower detection limit of 4.7 × 10 −8 mol L −1 (Table 5, entry 6).…”

Section: Go In Fluorescence Sensors For Natural Methylxanthinesmentioning

confidence: 99%

“…The growing popularity of these nanomaterials stimulated the synergistic use of many different methods for this purpose [21,22]. Electron microscopy methods enable morphological and structural characterization [23]: scanning electron microscopy (SEM) for the morphological and dimensional analysis of flakes, and transmission electron microscopy (TEM), scanning TEM (STEM), and high-resolution TEM (HRTEM) for the identification of GO sheets [21,24,25].…”

Section: Introductionmentioning

confidence: 99%

“…Chemical structures of these materials can be analyzed through solid-state nuclear magnetic resonance (SSNMR) spectroscopy [27], while atomic force microscopy (AFM) has been used for the visualization and accurate thickness evaluation of isolated flakes on substrates [24,25]. Moreover, advanced AFM-based methods can be used to study the mechanical properties of GO and RGO (e.g., AFM-based nanoindentation for the study of Young’s modulus and hardness of RGO [28]).…”

Section: Introductionmentioning

confidence: 99%

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Graphene Oxide: A Smart (Starting) Material for Natural Methylxanthines Adsorption and Detection

et al. 2019

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Natural methylxanthines, caffeine, theophylline and theobromine, are widespread biologically active alkaloids in human nutrition, found mainly in beverages (coffee, tea, cocoa, energy drinks, etc.). Their detection is thus of extreme importance, and many studies are devoted to this topic. During the last decade, graphene oxide (GO) and reduced graphene oxide (RGO) gained popularity as constituents of sensors (chemical, electrochemical and biosensors) for methylxanthines. The main advantages of GO and RGO with respect to graphene are the easiness and cheapness of synthesis, the notable higher solubility in polar solvents (water, among others), and the higher reactivity towards these targets (mainly due to π–π interactions); one of the main disadvantages is the lower electrical conductivity, especially when using them in electrochemical sensors. Nonetheless, their use in sensors is becoming more and more common, with the obtainment of very good results in terms of selectivity and sensitivity (up to 5.4 × 10−10 mol L−1 and 1.8 × 10−9 mol L−1 for caffeine and theophylline, respectively). Moreover, the ability of GO to protect DNA and RNA from enzymatic digestion renders it one of the best candidates for biosensors based on these nucleic acids. This is an up-to-date review of the use of GO and RGO in sensors.

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A fluorescence sensing platform of theophylline based on the interaction of RNA aptamer with graphene oxide

Abstract: A fluorescent sensing platform of theophylline based on the interaction of an RNA aptamer with GO and CdTe as the signal.

Cited by 16 publications

References 29 publications

Human virus detection with graphene-based materials

Human virus detection with graphene-based materials

Paper-based analytical devices for virus detection: Recent strategies for current and future pandemics

Graphene Oxide: A Smart (Starting) Material for Natural Methylxanthines Adsorption and Detection

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