A Review of Holocellulase Production Using Pretreated Lignocellulosic Substrates

Silva, Caio de Oliveira Gorgulho; Filho, Edivaldo Ximenes Ferreira

doi:10.1007/s12155-017-9815-x

Cited by 26 publications

(9 citation statements)

References 71 publications

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“…The unexpectedly higher peak of SF3.64 sample in relation to SF1.79 may be because of lignin redeposition from the harsh pretreatment. The change in the shape of the spectra around 800-1100 cm −1 , which are attributed to the C-O/C-H stretching of cellulose sugars and C-O-C stretching of β- (1,4) glycosidic bond of adjacent sugars [39], may be related to the increased exposure of carbohydrates following the removal of lignin by NaOH. These results indicate that the different pretreatment severities had different effects on the structure of the SCB, with varying impacts on the production of cellulase.…”

Section: Ftirmentioning

confidence: 99%

“…Raw SCB was treated under a combination of different NaOH concentrations, temperatures, and residence times, corresponding to three different severity factors (Table 1). The severity factors (SF) were calculated based on Equation (1). Slurries from the various pretreatment severities were processed, and the solids retained for use as carbon sources in shake flask fermentation.…”

Section: Effect Of Pretreatment Severity On Cellulase Productionmentioning

confidence: 99%

“…Cellulose and hemicellulose, which are polymeric structures within the lignocellulosic matrix, can be hydrolyzed into simple sugars for downstream fermentation or diversion to other purposes. However, the use of lignocellulosic as raw materials for biofuels and biochemicals is constrained by the high cost of hydrolytic enzymes, mainly cellulases, that are required for the hydrolysis of the complex polysaccharides into utilizable sugars [1]. Hence, one means of achieving the economic viability of biorefineries is to reduce the cost of cellulase [2].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of pretreatment severity on fungal cellulase production on sugarcane bagasse substrate

Kazeem

Uthman-Saheed

Oke

2021

Journal of Taibah University for Science

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On-site production of cellulases using lignocellulosic materials can improve the economic viability of biorefineries. This, however, requires the pretreatment of substrates using thermochemical conditions that can vary in severity. To understand the effect of pretreatment severity on cellulase production by Aspergillus ustus S3 on sugarcane bagasse, we applied NaOH pretreatment corresponding to 3 severity factors (SF1.32, SF1.79, and SF3.64) to generate SCB that was used as inducing substrate. The highest cellulase activity (0.681 U/mL) was obtained with the intermediate severity (SF1.79) while significantly lower activities of 0.495 and 0.539 U/mL were recorded with low (SF1.32) and high (SF3.64) severities, respectively. Chemical and structural characterization revealed that low and intermediate severities improved cellulose accessibility and cellulase titres while high severity impaired them, thus limiting substrate suitability for enzyme production. These results show that though high SFs might be desirable in other applications, moderate severities may be more appropriate for cellulase production.

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Section: Ftirmentioning

confidence: 99%

Section: Effect Of Pretreatment Severity On Cellulase Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of pretreatment severity on fungal cellulase production on sugarcane bagasse substrate

Kazeem

Uthman-Saheed

Oke

2021

Journal of Taibah University for Science

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“…To avoid the contamination of the resulting mixtures with undesired hydrolysis products, alternative methods for the delignification of Miscanthus are being actively developed. Specifically, recent reports describe the use of formic/acetic acid [6], microwave heating [7,8], and hydrogen peroxide/formic acid [9] as pretreatment agents. Despite these facts, the described protocols for the production of ethanol from different Miscanthus spp.…”

Section: Introductionmentioning

confidence: 99%

The Process of Producing Bioethanol from Delignified Cellulose Isolated from Plants of the Miscanthus Genus

et al. 2020

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Plants of the Miscanthus genus (Miscanthus Anderss.) have a unique index of biomass production in relation to the occupied area. Miscanthus plants can be attributed to promising second-generation raw materials for the production of bioethanol and biofuel. Miscanthus plants are characterized by a high cellulose content. Herein, we report the results of a study on the obtained delignified cellulose with subsequent processing into bioethanol using microbial communities. In the course of the study, the optimal conditions for the delignification of the initial plant material for cellulose were selected. Ethanol with a high degree of conversion was successfully obtained from the isolated delignified cellulose. The article describes the pilot technological scheme for the conversion of Miscanthus plant biomass to bioethanol involving the delignification stages, followed by the conversion of the resulting cellulose into bioethanol by a consortium of microorganisms. As a result of the study, it was found that delignification using trifluoroacetic acid leads to the production of cellulose of high purity. Bioethanol with a yield of 3.1% to 3.4% in terms of the initial amount of biomass was successfully obtained by a microorganism consortium of Saccharomyces cerevisiae M Y-4242/Pachysolen tannophilus Y-3269, and Scheffersomyces stipitis Y-3264.

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“…Lignocellulosic biomass is a significant renewable resource available worldwide for the production of sustainable biofuels and bioproducts at competitive prices (Wen et al 2013;Liu et al 2015). A large market potential for cellulase production is projected due to its capability of converting lignocellulosic biomass to fermentable sugars for the production of high value chemicals, which has required faster methods to improve its productivity (Silva and Filho 2017).…”

Section: Introductionmentioning

confidence: 99%

Production of cellulase by Microbulbifer hydrolyticus through co-fermentation of glucose and xylose from lignocellulose

Liu

Xiao

et al. 2020

BioRes

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Cellulase is a compound enzyme that catalyzes cellulose into monosaccharides or oligosaccharides. Large amounts of cellulase are needed with the development of the lignocellulose processing industry, which necessitates faster methods to produce cellulase. In this work, the marine bacterium Microbulbifer hydrolyticus IRE-31-192 was selected to produce cellulase, due to its fast growth rate and short high space-time yield. Co-fermentation of glucose and xylose to produce cellulase was investigated on the basis of previous work. When the ratio of glucose/xylose was 2:1 (w/w), 294 U/L cellulase activity with highest space-time yield of 12.2 U/L h was obtained. The hydrolytic liquid of lignocellulose prepared from dried distiller’s grains with solubles (DDGS) with the similar ratio of glucose/xylose was used as medium to produce cellulase. The efficiency of cellulase production from processed and unprocessed hydrolysates of DDGS was compared. Unprocessed hydrolysates were more beneficial for the production of cellulase, such that its activity was 261 U/L with a space-time yield of 14.5 U/L h. Thus, commonly used pure glucose and xylose could be replaced by hydrolysates of DDGS, and marine bacteria has potential application for cellulase production.

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A Review of Holocellulase Production Using Pretreated Lignocellulosic Substrates

Cited by 26 publications

References 71 publications

Impact of pretreatment severity on fungal cellulase production on sugarcane bagasse substrate

Impact of pretreatment severity on fungal cellulase production on sugarcane bagasse substrate

The Process of Producing Bioethanol from Delignified Cellulose Isolated from Plants of the Miscanthus Genus

Production of cellulase by Microbulbifer hydrolyticus through co-fermentation of glucose and xylose from lignocellulose

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